Retroviral endostatin gene transfer inhibits growth of human lung cancer in a murine orthotopic xenotransplant model

Kurdow, Roland; Boehle, Arnd S; Ruhnke, Maren; Mendoza, Renata; Boenicke, Lars; Sipos, Bence; Schniewind, Bodo; Dohrmann, P.; Kalthoff, Holger

doi:10.1007/s00423-003-0400-8

Cited by 6 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are in line with experimental studies on other cancer subtypes in which endostatin suppresses tumor growth. [3][4][5]8,16,23,26,30,33,35,[37][38][39]48 In contrast to previous work, however, we did not observe significantly prolonged survival or tumor volume reduction after treatment with human endostatin. The reason for this discrepancy is unclear.…”

Section: Discussioncontrasting

confidence: 74%

Antitumor efficacy improved by local delivery of species-specific endostatin

Huszthy

Brekken²,

Pedersen³

et al. 2006

JNS

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 74%

Antitumor efficacy improved by local delivery of species-specific endostatin

Huszthy

Brekken²,

Pedersen³

et al. 2006

JNS

View full text Add to dashboard Cite

show abstract

“…However, as this fragment is thought to exert its antiangiogenic function by interacting with integrin a1b1 [27], it is an unlikely candidate for inhibition of tumor angiogenesis in the integrin a1-null mice. In addition to the collagen IV NC1 domains, it has been demonstrated that lung tumors derived from NSCLC cells engineered to secrete endostatin are smaller and less metastatic than tumors derived from control NSCLC cells [28]. Although endostatin can be generated from purified collagen XVIII by MMP7 [29] and a1-null endothelial cells produce more MMP7 than their wild type counterparts [6], we have not explored the contribution of this molecule in our mouse model.…”

Section: Orthotopic Model Of Nsclcmentioning

confidence: 96%

An Orthotopic Model of Lung Cancer to Analyze Primary and Metastatic NSCLC Growth in Integrin α1-Null Mice

Chen

Fingleton

et al. 2005

Clin Exp Metastasis

View full text Add to dashboard Cite

The role of matrix metalloproteinase (MMP)9 in lung cancer progression is controversial. MMP9 promotes local tumor progression and distant metastasis in mouse models by enhancing extracellular matrix degradation, releasing VEGF from extracellular matrix and promoting vascular pericyte recruitment. Furthermore, increased plasma MMP9 expression levels in human subjects with metastatic non-small cell lung cancer (NSCLC) inversely correlates with survival. In contrast, MMP9 can benefit the host by generating inhibitors of endothelial cell proliferation such as angiostatin and NC1 domains of collagen IV. To better understand the role of host MMP9 on the primary growth and metastatic potential of NSCLC, we performed an orthotopic model of NSLC in integrin alpha1-null mice (a genetic model for increased MMP9). In these mice we observed decreased number, size and vascularization of primary NSCLC tumors when compared to wild type controls. In addition, decreased number and size of NSCLC-derived metastases were evident in the alpha1-null mice. Furthermore, pharmacological inhibition of MMPs in the alpha1-null mice at the time of tumor cell injection resulted in an increase in the number of both primary and metastatic lung cancer as compared to untreated mice, suggesting that primary growth and metastases of NSCLC are worsened by the early inhibition of MMPs. In conclusion, although MMP9 may potentially promote tumor growth and metastasis, production of MMP-dependent anti-angiogenic factors seems to override these effects and protects the host from NSCL growth and progression.

show abstract

“…Different gene constructs have been used in different cancer model by intratumor injection [24], subcutaneous administration [25] or low voltage electroporation [26]. The endostatin gene was even retrovirally transduced to tumor cells and the transduced cells were subsequently xenotransplanted in a murine tumor model [27]. Kurosaka et al [28] reported that systemic administration of endostatin can inhibit pannus formation and bone destruction in arthritis.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of corneal neovascularization with endostatin delivered by adeno-associated viral (AAV) vector in a mouse corneal injury model

Lai

Xiao

2007

J Biomed Sci

View full text Add to dashboard Cite

The use of a recombinant adeno-associated viral (rAAV) vector carrying endostatin gene as an anti-angiogenesis strategy to treat corneal neovascularization in a mouse model was evaluated. Subconjunctival injection of recombinant endostatin-AAV was used to examine the inhibition of corneal neovascularization induced by silver nitrate cauterization in mice. The results showed that gene expression in corneal tissue was observed as early as 4 days after gene transfer and stably lasted for over 8 months with minimal immune reaction. Subconjunctival injection of a high-titer rAAV-endostatin successfully inhibited neovascularization. Immunohistchemistry staining of CD 31 and endostatin showed that the treatment significantly inhibits angiogenesis in cornea. We concluded that the rAAV was capable of directly delivering genes to the ocular surface epithelium by way of subconjunctival injection and was able to deliver sustained, high levels of gene expression in vivo to inhibit angiogenesis.

show abstract

Retroviral endostatin gene transfer inhibits growth of human lung cancer in a murine orthotopic xenotransplant model

Abstract: Retroviral transduction of the human endostatin gene is capable of achieving long-term endostatin expression. Endostatin transduction provides significant anti-tumor effects with regard to local tumor growth, metastases and survival.

Cited by 6 publications

References 20 publications

Antitumor efficacy improved by local delivery of species-specific endostatin

Antitumor efficacy improved by local delivery of species-specific endostatin

An Orthotopic Model of Lung Cancer to Analyze Primary and Metastatic NSCLC Growth in Integrin α1-Null Mice

Inhibition of corneal neovascularization with endostatin delivered by adeno-associated viral (AAV) vector in a mouse corneal injury model

Contact Info

Product

Resources

About