Retrotransposon based genetic status of North-West Himalayan Zingiber officinale revealed high heterogeneity

Pandotra, Pankaj; Husain, Mohd Kashif; Ram, G.; Gupta, Suphla; Gupta, Ajai Prakash

doi:10.1007/s13562-013-0196-8

Cited by 6 publications

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“…Second, our SSR and ISSR primers, selected from the studies of Pandotra et al (2014) and Baruah et al (2019), were also proved as efficient primers for ginger genetic diversity analysis. Specifically, Pandotra et al (2014) concluded that RT-6/RM-2, RT-6/RM-3, RT-6/RM-4, RT-6/RM-125, and RT-6/RM-130 were among the best primer pairs, with PIC, Rp,MI,Np,[17][18][19][20][21][22][23]respectively. Additionally, were also the optimal ISSR primers in the study of Baruah et al (2019), with the Np, %P, MI,Rp,, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Ten REMAP primer pairs (Baruah et al, 2019;Pandotra et al, 2014) were screened (Table 2). Each tested primer pair was used to amplify seven randomly chosen DNA samples from distinct regions.…”

Section: Primer Selectionmentioning

confidence: 99%

“…Retrotransposon microsatellite amplification polymorphism (REMAP) is an efficient technique involving the polymerase chain reaction (PCR) amplification, with a long terminal repeat (LTR) primer and a simple sequence repeat (SSR) or inter-simple sequence repeat (ISSR) primer (Kalendar and Schulman, 2006;Pandotra et al, 2014). REMAP has been efficiently used for cultivar characterizations of many species, including barley (Hordeum vulgare), grapevine (Vitis vinifera L.), olive (Olea europaea L.), and rice (Oryza sativa L.) (Branco et al, 2007;Kalendar et al, 1999;Kaya and Yilmaz-Gokdogan, 2016;Strioto et al, 2019;Šimon et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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2022

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell a n d tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscripts that meet the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately in one to two months after acceptance.

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Section: Discussionmentioning

confidence: 99%

Section: Primer Selectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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“…En algunos casos, una clara diferenciación genética se observó entre variedades comerciales o mejoradas y variedades tradicionales o locales (Kizhakkayil y Sasikumar, 2010;Pandotra et al, 2014;Prem et al, 2008), indicándose como materiales potenciales para programas de mejoramiento por su alta calidad (Sajeev et al, 2011). La variabilidad en cultivares de Z. officinale tiene una connotación evolutiva.…”

Section: Diversidad Genética a Nivel Molecularunclassified

“…No obstante, dos técnicas basadas en el uso de retrotransposones (IRAP: "Inter Retrotransposons Amplified Polymorphism" y REMAP: "Retrotransposon Microsatellite Amplified Polymorphism"), han sido usadas recientemente para el análisis de la diversidad genética en jengibre (Pandotra et al, 2015). IRAP y REMAP fueron eficientes en el análisis genético de Z. officinale, revelando más de 90% de polimorfismo entre 92 variedades locales colectadas en la India, en el noroeste de la región del Himalaya (Pandotra et al, 2014). De acuerdo con los autores, la alta amplificación de locus REMAP e IRAP, con gran polimorfismo, es un indicativo de variaciones ocurridas en los…”

Section: Diversidad Genética a Nivel Molecularunclassified

Diversidad genética del jengibre ( Zingiber officinale Roscoe . ) A nivel molecular: Avances de la última década

Zambrano-Blanco

2015

ENTRAMADO

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(CIAB). Universidad Nacional Abierta y a Distancia (UNAD). Dosquebradas, Risaralda -Colombia. ezambranob@yahoo.es R e s u m e nEl jengibre (Zingiber officinale) es una especie medicinal, aromática y condimentaria que se cultiva principalmente en regiones tropicales y subtropicales del mundo. Por tratarse de una especie de propagación vegetativa, su variabilidad genética tiende a ser baja; sin embargo es posible hallar una amplia variabilidad genética principalmente en la China e India, considerándose actualmente como el principal centro de origen y diversidad de esta especie. Conocer y caracterizar la diversidad genética del jengibre es una tarea de vital importancia para fomentar programas de conservación ex situ o in vitro que ayuden a evitar la erosión genética de esta especie, así como para direccionar correctamente estrategias de mejoramiento genético. Marcadores moleculares han sido ampliamente usados en los últimos años con la finalidad de estudiar la variabilidad genética del jengibre, directamente a nivel del ADN. En este artículo, los avances en la investigación en diversidad genética del jengibre, usando marcadores moleculares, son revisados. Esto con la finalidad de hacer un análisis sobre sus implicaciones para la conservación y el mejoramiento genético de la especie. PalabRas c l av eMarcadores moleculares, recursos fitogenéticos, mejoramiento genético, relaciones filogenéticas, Zingiber officinale Genetic diversity of ginger (Zingiber officinale Roscoe.) At the molecular level: progress of the last decade a b s t R a c t Ginger (Zingiber officinale) is a medicinal, aromatic and spice species grown in tropical and subtropical regions, mainly. Being a vegetatively propagated species, their genetic diversity tends to be low; however, wide genetic variability is possible to found it in China and India, especially. Actually, these countries are considered as the center of origin and diversity of ginger. To know and to characterize the genetic diversity of ginger is an activity of vital importance to promote ex situ and in vitro conservation programs, to help prevent genetic erosion of this species as well address breeding strategies properly. Molecular markers have been widely used in recent years in order to study the genetic variability of ginger, directly at the DNA level. In this paper, the research advances in genetic diversity of ginger, using molecular markers, are reviewed. This in order to analyze their implications for the conservation and breeding of the species. Cómo citar este artículo: ZAMBRANO-BLANCO, Eleonora. Diversidad genética del jengibre (zingiber officinale Roscoe.) A nivel molecular: Avances de la última década. En: Entramado.

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Molecular markers: a potential resource for ginger genetic diversity studies

et al. 2016

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Ginger is an economically important and valuable plant around the world. Ginger is used as a food, spice, condiment, medicine and ornament. There is available information on biochemical aspects of ginger, but few studies have been reported on its molecular aspects. The main objective of this review is to accumulate the available molecular marker information and its application in diverse ginger studies. This review article was prepared by combing material from published articles and our own research. Molecular markers allow the identification and characterization of plant genotypes through direct access to hereditary material. In crop species, molecular markers are applied in different aspects and are useful in breeding programs. In ginger, molecular markers are commonly used to identify genetic variation and classify the relatedness among varieties, accessions, and species. Consequently, it provides important input in determining resourceful management strategies for ginger improvement programs. Alternatively, a molecular marker could function as a harmonizing tool for documenting species. This review highlights the application of molecular markers (isozyme, RAPD, AFLP, SSR, ISSR and others such as RFLP, SCAR, NBS and SNP) in genetic diversity studies of ginger species. Some insights on the advantages of the markers are discussed. The detection of genetic variation among promising cultivars of ginger has significance for ginger improvement programs. This update of recent literature will help researchers and students select the appropriate molecular markers for ginger-related research.

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Retrotransposon based genetic status of North-West Himalayan Zingiber officinale revealed high heterogeneity

Cited by 6 publications

References 11 publications

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Diversidad genética del jengibre ( Zingiber officinale Roscoe . ) A nivel molecular: Avances de la última década

Molecular markers: a potential resource for ginger genetic diversity studies

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