Retrospect and prospects of plant metabolic engineering

Chownk, Manisha; Thakur, Karnika; Yadav, Sudesh Kumar

doi:10.1007/s13562-018-0473-7

Cited by 9 publications

(4 citation statements)

References 61 publications

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“…Metabolic engineering holds a promising hope for the viable systematization of production of biologically active compounds that are necessary for human health and generation of improved crop varieties involving the redirection of enzymatic reaction(s) in endogenous pathways to synthesize novel products or enrich the existing ones [52][53][54][55]. Two approaches employed for the functional gene studies in plants are stable and transient expression.…”

Section: Discussionmentioning

confidence: 99%

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Malla

Shanmugaraj

Sharma

et al. 2021

Plants

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Isoflavonoids, the diverse group of secondary metabolites derived from the phenylpropanoid pathway, are distributed predominantly in leguminous plants. It has received considerable attention in recent days due to its health promoting benefits and is known to prevent certain diseases in humans. These isoflavonoids are synthesized from flavonoid intermediates of phenylpropanoid pathway by the enzyme isoflavone synthase. Metabolic engineering of isoflavonoid biosynthesis in non-legume crop plants could offer the health benefits of these compounds in diverse plant species further contributing for crop improvement. The transient expression of heterologous genes in the host is considered as an alternative to stable expression, that can provide a rapid way of studying the pathway engineering for metabolite production and could also act as a production platform for nutraceuticals and biopharmaceuticals. In this study, isoflavone genistein was produced in Amaranthus tricolor var. tristis and Spinacia oleracea by transiently expressing Glycine max isoflavone synthase (GmIFS). The GmIFS gene was cloned in plant expression vector pEarleyGate 102 HA and pEAQ-HT-DEST 3 and transformed into plants by agroinfiltration. The presence of transgene in the agroinfiltrated leaves was confirmed by semiquantitative reverse-transcription polymerase chain reaction. The flavonoid substrate naringenin and isoflavonoid genistein were quantified using high performance liquid chromatography in both wild-type and infiltrated leaf samples of both the plants. The naringenin content varied in the range of 65.5–338.5 nM/g fresh weight, while the accumulation of genistein was observed with varying concentrations from 113 to 182.6 nM/g fresh weight in the agroinfiltrated leaf samples of both A. tricolor var. tristis and S. oleracea. These results indicate that the transient expression of GmIFS gene has led to the synthesis of isoflavonoid genistein in A. tricolor var. tristis and S. oleracea providing an insight that stable expression of this gene could enrich the nutraceutical content in the crop plants. To the best of our knowledge, this is the first report on transient expression of GmIFS gene for the production of genistein in A. tricolor var. tristis and S. oleracea.

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Section: Discussionmentioning

confidence: 99%

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Malla

Shanmugaraj

Sharma

et al. 2021

Plants

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“…Downregulation of miRNAs can be achieved by artificial micro RNAs and synthetic transacting siRNAs [154-156] Metabolic engineering involves manipulating complex biosynthetic pathways to increase production rate of novel beneficial metabolites in plants and microbes. such as golden rice, artemisinin, and flavonoids, and to improve tolerance to stress in plants while reducing harmful metabolites in specific crop plants [157,158]. By using plant miRNA gene regulator tools, researchers can gain better understanding of biosynthetic pathways and modify them to achieve desired characteristics [159].…”

Section: Metabolic Engineeringmentioning

confidence: 99%

Metabolomics and Metabolic Engineering for Crop Improvement: Current Trends and Future Prospects

Singh,

Karadagi,

Doggalli

et al. 2024

PCBMB

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Metabolomics is a rising field within the realm of “omics,” focusing on the detection and measurement of metabolites and chemical markers associated with cellular regulatory mechanisms across various biological organisms. The exploration of metabolomic control in plant life plays a imperative role in comprehending their ability to adapt, acclimate, and defend against environmental pressures by generating a diverse array of metabolites. Furthermore, the application of metabolomics holds promise in the characterization of plant traits, offering significant prospective for amalgamation into genome editing initiatives aimed at advancing the development of enhanced, future-generation crops. The forefront technologies have introduced economical and high-capacity methods to molecularly analyze the operation of cells or organisms. Cutting-edge analytical methods in metabolomics, such as nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectrometry (LC-MS), gas chromatography-mass (GC-MS) and high performance liquid chromatography (HPLC) have accelerated metabolic profiling. This review provides an insights into the latest tools in plant metabolomics for enhancing crops and process of plant metabolome research, engaging in plant mechanisms especially for tolerating biotic and abiotic stresses. This review also provide potential approaches to metabolomics through metabolic engineering such as miRNA- and RNAi-Mediated Metabolic Engineering, Genome editing mediated metabolic Engineering etc

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“…Up to now, two common systems have been used for gene transfer in the generation of GM plants: the Agrobacterium-mediated system and the gene-gun-mediated system [25,26]. With the development of transgenic technology, genetic modification has been widely used to verify the gene functions in the flavonoid biosynthesis pathway, and the roles of flavonoids in plant development and response to (a)biotic stresses [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

The Classification, Molecular Structure and Biological Biosynthesis of Flavonoids, and Their Roles in Biotic and Abiotic Stresses

Zhuang

Shu

et al. 2023

Molecules

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With the climate constantly changing, plants suffer more frequently from various abiotic and biotic stresses. However, they have evolved biosynthetic machinery to survive in stressful environmental conditions. Flavonoids are involved in a variety of biological activities in plants, which can protect plants from different biotic (plant-parasitic nematodes, fungi and bacteria) and abiotic stresses (salt stress, drought stress, UV, higher and lower temperatures). Flavonoids contain several subgroups, including anthocyanidins, flavonols, flavones, flavanols, flavanones, chalcones, dihydrochalcones and dihydroflavonols, which are widely distributed in various plants. As the pathway of flavonoid biosynthesis has been well studied, many researchers have applied transgenic technologies in order to explore the molecular mechanism of genes associated with flavonoid biosynthesis; as such, many transgenic plants have shown a higher stress tolerance through the regulation of flavonoid content. In the present review, the classification, molecular structure and biological biosynthesis of flavonoids were summarized, and the roles of flavonoids under various forms of biotic and abiotic stress in plants were also included. In addition, the effect of applying genes associated with flavonoid biosynthesis on the enhancement of plant tolerance under various biotic and abiotic stresses was also discussed.

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Retrospect and prospects of plant metabolic engineering

Cited by 9 publications

References 61 publications

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase

Metabolomics and Metabolic Engineering for Crop Improvement: Current Trends and Future Prospects

The Classification, Molecular Structure and Biological Biosynthesis of Flavonoids, and Their Roles in Biotic and Abiotic Stresses

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