Retraction Note to: First report of the Phe1534Cys kdr mutation in natural populations of Aedes albopictus from Brazil

Aguirre-Obando, Oscar Alexander; Martins, Ademir Jesús; Navarro-Silva, Mário Antônio

doi:10.1186/s13071-022-05382-9

Cited by 2 publications

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“…Reports on Brazilian Ae. aegypti populations indicate a high frequency of both V1016I and F1534C substitutions in comparison to susceptible alleles [36,40,64,68,69]. Thus, the enzymatic activity variations and kdr mutations observed in Aedes Rio were inherited characteristics from the mosquito populations collected from Rio de Janeiro and used for interbreeding, leading to the enhanced RR observed herein.…”

Section: Discussionmentioning

confidence: 71%

“…Several studies have correlated MFO activity or expression levels of the P450 cyp family genes to increased pyrethroid resistance; however, so far there is no particular marker available for large-scale analysis [55][56][57][58]. On the other hand, genotyping of specific kdr mutations has also been well correlated to pyrethroid resistance, and are thus currently widely used in molecular surveillance of insecticide resistance [28,[59][60][61][62][63][64]. No altered MFO concentrations were observed herein.…”

Section: Discussionmentioning

confidence: 91%

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Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil

et al. 2020

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Background Aedes aegypti is a vector of high relevance, since it transmits several arboviruses, including dengue, chikungunya and Zika. Studies on vector biology are usually conducted with laboratory strains presenting a divergent genetic composition from field populations. This may impair vector control policies that were based on laboratory observations employing only long maintained laboratory strains. In the present study we characterized a laboratory strain interbreed with Ae. aegypti collected from five different localities in Rio de Janeiro (Aedes Rio), for insecticide resistance (IR), IR mechanisms, fitness and Zika virus infection. Methods We compared the recently established Aedes Rio with the laboratory reference strain Rockefeller. Insecticide resistance (deltamethrin, malathion and temephos), activity of metabolic resistance enzymes and kdr mutation frequency were determined. Some life table parameters (longevity, blood-feeding, number and egg viability) and Zika virus susceptibility was also determined. Results Aedes Rio showed resistance to deltamethrin (resistance ratio, RR50 = 32.6) and temephos (RR50 = 7.0) and elevated activity of glutathione S-transferase (GST) and esterases (α-EST and pNPA-EST), but not acetylcholinesterase (AChE). In total, 92.1% of males genotyped for kdr presented a “resistant” genotype. Weekly blood-fed females from both strains, presented reduced mortality compared to sucrose-fed mosquitoes; however, Aedes Rio blood-fed females did not live as long (mean lifespan: Rockefeller = 70 ± 3.07; Aedes Rio = 53.5 ± 2.16 days). There were no differences between strains in relation to blood-feeding and number of eggs, but Aedes Rio eggs presented reduced viability (mean hatch: Rockefeller = 77.79 ± 1.4%; Aedes Rio = 58.57 ± 1.77%). Zika virus infection (plaque-forming unit, PFU) was similar in both strains (mean PFU ± SE: Aedes Rio: 4.53 × 104 ± 1.14 × 104 PFU; Rockefeller: 2.02 × 104 ± 0.71 × 104 PFU). Conclusion Selected conditions in the field, such as IR mechanisms, may result in pleiotropic effects that interfere in general physiology of the insect. Therefore, it is important to well characterize field populations to be tested in parallel with laboratory reference strains. This practice would improve the significance of laboratory tests for vector control methods.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 91%

Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil

et al. 2020

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“…During the year 2017-2018, studies from China and Brazil further support the importance of the F1534C and F1534L kdr mutation in the evolution of pyrethroid resistance in the wild population of Ae. albopictus 2,16 . Recent studies in China further added V1016G mutation in the IIS6 region of vgsc gene denotes spreading of kdr mutation in the vgsc gene of Ae.…”

Section: First Report Of F1534c Kdr Mutation In Deltamethrin Resistan...mentioning

confidence: 99%

“…PCR reaction was carried out for the partial amplification of IIIS6 vgsc gene with a expected length of 350 bp. Primers used for these reactions were AaEx31P (5′-TCG CGG GAG GTA AGT TAT TG-3′) and AaEx31Q (5′-GTT GAT GTG CGA TGG AAA TG-3′) 16 . Reaction was carried out with 1X Go®Taq G2 Green Master Mix (Promega, USA) of 12.5 μl, 1 μl of both forward and reverse primers, 2 μl of template DNA and 8.5 μl of nuclease free water in 25 μl reaction mixture.…”

Section: Study Area and Sample Collection Dengue Endemic Darjeeling D...mentioning

confidence: 99%

First report of F1534C kdr mutation in deltamethrin resistant Aedes albopictus from northern part of West Bengal, India

Modak

Saha

2022

Sci Rep

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Dengue is the most rapidly spreading vector-borne disease with an estimated 100–400 million cases each year. Control of Dengue vectors largely depends upon synthetic pyrethroids. Development of insecticide resistance in Aedes mosquitoes however, poses severe threat to insecticide-based vector management programme. Mutations in the Voltage Gated Sodium Channel gene (vgsc) serve as the primary machinery behind this resistance development. In Aedes albopictus, at least four such kdr (knock down resistance) mutations had already been documented. Here, we describe the occurrence of F1534C kdr mutation in wild population of Ae. albopictus from northern part of West Bengal, India including a novel T1520I mutation. Four populations of Ae. albopictus from the studied region were found resistant against DDT and synthetic pyrethroids, among them only one population possessed F1534C kdr mutation. A total of 200 successful amplification followed by partial sequencing of vgsc gene further revealed the presence of F1534C kdr mutation in both phenotypically susceptible and resistant mosquito specimen. Studied populations were found 81% homozygote susceptible (1534F/F), 12.5% heterozygote (1534F/C) and 6% homozygote resistant (1534C/C) for F1534C kdr mutation. The findings of the current study will help to uncover the mechanisms underlying insecticide resistance and hence to reduce errors in vector control measurements.

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Retraction Note to: First report of the Phe1534Cys kdr mutation in natural populations of Aedes albopictus from Brazil

Cited by 2 publications

References 0 publications

Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil

Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil

First report of F1534C kdr mutation in deltamethrin resistant Aedes albopictus from northern part of West Bengal, India

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