Retraction Note: Drying of a plasmid containing formulation: chitosan as a protecting agent

Mohajel, Nasir; Najafabadi, Abdolhossein R.; Azadmanesh, Kayhan; Vatanara, Alireza; Amini, Mohsen; Moazeni, Esmail; Rahimi, Amir Abbas; Gilani, Kambiz

doi:10.1186/s40199-016-0150-0

Cited by 1 publication

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the circulation half-life of BMP-2 is rather short. BMP-2 is easily to be inactivated due to dilution or interaction with enzymes in blood if applied alone by intravenous injection ( 20 , 21 ). Another drawback is that the intravenous injection of BMP-2 alone may produce burst effect, which may lead to soft tissue hematoma and bone absorption phenomenon ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

Efficient delivery of recombinant human bone morphogenetic protein (rhBMP‑2) with dextran sulfate‑chitosan microspheres

Xia

Ling

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

Bone morphogenetic protein-2 (BMP-2) serves an important role in the development of bone and cartilage. However, administration of BMP-2 protein alone by intravenous delivery is not very effective. Sustained delivery of stabilized BMP-2 by carriers has been proven necessary to improve the osteogenesis effect of BMP-2. The present study constructed a novel drug delivery system using dextran sulfate (DS)-chitosan (CS) microspheres and investigated the efficiency of the delivery system on recombinant human bone morphogenetic protein (rhBMP-2). The microsphere morphology, optimal ratio of DS/CS/rhBMP-2, and drug loading rate and entrapment efficiency of rhBMP-2 CS nanoparticles were determined. L929 cells were used to evaluate the cytotoxicity and effect of DS/CS/rhBMP-2 microspheres on cell proliferation. Differentiation study was conducted using bone marrow mesenchymal stem cells (BMSCs-C57) cells treated with DS/CS/rhBMP-2 microspheres or the control microspheres. The DS/CS/rhBMP-2 microspheres delivery system was successfully established. Subsequent complexation of rhBMP-2-bound DS with polycations afforded well defined microspheres with a diameter of ~250 nm. High protein entrapment efficiency (85.6%) and loading ratio (47.245) µg/mg were achieved. Release of rhBMP-2 from resultant microspheres persisted for over 20 days as determined by ELISA assay. The bioactivity of rhBMP-2 encapsulated in the CS/DS microsphere was observed to be well preserved as evidenced by the alkaline phosphatase activity assay and calcium nodule formation of BMSCs-C57 incubated with rhBMP-2-loaded microspheres. The results demonstrated that microspheres based on CS-DS polyion complexes were a highly efficient vehicle for delivery of rhBMP-2 protein. The present study may provide novel orientation for bone tissue engineering for repairing and regenerating bone defects.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient delivery of recombinant human bone morphogenetic protein (rhBMP‑2) with dextran sulfate‑chitosan microspheres

Xia

Ling

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

show abstract

Retraction Note: Drying of a plasmid containing formulation: chitosan as a protecting agent

Abstract: This article [1] has been retracted by the publisher because it was republished in the journal [2] due to an error during the process of journal transfer between publishers. BioMed Central apologizes to the authors and to readers for this error and for any inconvenience caused.

Cited by 1 publication

References 2 publications

Efficient delivery of recombinant human bone morphogenetic protein (rhBMP‑2) with dextran sulfate‑chitosan microspheres

Efficient delivery of recombinant human bone morphogenetic protein (rhBMP‑2) with dextran sulfate‑chitosan microspheres

Contact Info

Product

Resources

About