RETRACTED: Total glucosides of paeony suppresses experimental autoimmune uveitis in association with inhibition of Th1 and Th2 cell function in mice

Abstract

Total glucosides of paeony (TGP) are active components extracted from the roots of Paeonia lactiflora Pall. In this study, we investigated the role and mechanisms of TGP in experimental autoimmune uveitis (EAU) model of mice. The C57BL/6 mice were randomly divided into three groups: sham group, EAU-control group, and EAU-TGP group. Clinical score of images of the eye fundus were taken on 7, 14, 21, and 28 days after induction of EAU. The concentrations of proinflammatory cytokines in intraocular fluid were measured at 14 days after EAU induction with the use of a multiplex assay system. Flow cytometry was used to analyze the frequency of CD4+, CD8+, interferon-gamma (IFN-γ), and CD4+/CD8+ ratio in spleen and lymph nodes. Western blotting was used to measure expressions of mitogen-activated protein kinase (MAPK) pathway-related proteins in retina. Clinical scores for uveitis were lower in TGP-treated EAU mice than those without TGP treatment. Importantly, the concentrations of cytokines induced by T-helper 1 (Th1) and T-helper 2 (Th2) cells in intraocular fluid were reduced in EAU mice treated with TGP. Furthermore, the frequency of CD4+, IFN-γ, and CD4+/CD8+ ratio was decreased and the frequency of CD8+ was increased in spleen and lymph nodes of mice treated with TGP. The anti-inflammatory effects of TGP were mediated by inhibiting the MAPK signaling pathways. Our results showed that TGP suppressed uveitis in mice via the inhibition of Th1 and Th2 cell function. Thus, TGP may be a promising therapeutic strategy for uveitis, as well as other ocular inflammatory diseases.

Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF-κB pathway

“…Loganic acid decreased proinflammatory cytokines in hypercholesterolemic rabbits [62,63] . Paeoniflorin is able to suppress inflammation in experimental arthritis by inhibiting abnormal proliferation of lymphocytes and synoviocytes and the production of proinflammatory cytokines and chemokines, nitric oxide, vascular endothelial growth factor (VEGF), and GM-CSF by synoviocytes [19,[64][65][66] . Ferulic acid, isolated from chuanxiong and danggui [67] has antioxidative and anti-inflammatory effects [67,68] .…”

Development of herbal molecules in treating autoimmune uveitis: a narrative review

“…A murine experiment investigated the effects of total glucosides of peony (TGP), which is extracted from the roots of Paeonia lactiflora Pall, on suppressing experimental AU. 68 TGP showed anti-inflammatory and immunomodulatory 70 The harmful effect of LXT is correlated with the cumulative dose of Radix bupleuri. Bupleurum might be a source of hepatotoxicity.…”

Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis

“…Total glucosides of paeony (TGP) is an active ingredient extracted from the root of Paeonia alba that is characterized by anti-inflammatory, anti-oxidative and pain-relieving properties ( 9 , 12 ). In addition, TGP regulates the balance of the T helper (h)1/Th2 ratio via two-way cellular immune regulation, which induces reverse modulation of B lymphocyte growth ( 13 ). TGP has been reported to serves an important role in the treatment of both inflammatory and immune disease ( 14 – 16 ).…”