RETRACTED: Theaflavins induce G2/M arrest by modulating expression of p21waf1/cip1, cdc25C and cyclin B in human prostate carcinoma PC-3 cells

Prasad, Sahdeo; Kaur, Jaspreet; Roy, Praveen K.; Kalra, Neetu; Shukla, Yogeshwer

doi:10.1016/j.lfs.2007.07.033

Cited by 52 publications

(42 citation statements)

References 40 publications

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“…Similarly, after treatment of stomach cancer cells SG-7901 with 125 µg/mL of green tea, black tea, or Pur tea extracts, or 50 µg/mL of catechin for 48 hours, the proportions of early apoptosis cells rose to 10.4%, 5.9%, 3.9%, and 12.6%, respectively, while the late apoptosis cells increased to 5.7%, 4.4%, 2.0%, and 8.3%, respectively. 54 Prasad et al 18 further reported that the …”

Section: Pc-3 Cell Apoptosis Analysismentioning

confidence: 98%

“…5 Similarly, after treatment of prostate cancer cell PC-3 with black tea extract (40-80 µg/mL), a dose-dependent inhibition was observed with the IC 50 being 60 µg/mL. 18 In addition, the expressions of P21, caspase-9, and caspase-3 were elevated, while the expressions of B-cell lymphoma 2 (bcl-2) and cyclin B declined, leading to cell cycle arrest at G2/M phase for subsequent apoptosis. 18 Thus, the objectives of our study were to develop a better HPLC-MS method for separation of eight catechin standards and quantitation of catechins in green tea leaf waste.…”

Section: Introductionmentioning

confidence: 95%

“…18 In addition, the expressions of P21, caspase-9, and caspase-3 were elevated, while the expressions of B-cell lymphoma 2 (bcl-2) and cyclin B declined, leading to cell cycle arrest at G2/M phase for subsequent apoptosis. 18 Thus, the objectives of our study were to develop a better HPLC-MS method for separation of eight catechin standards and quantitation of catechins in green tea leaf waste. Meanwhile, catechin nanoemulsion was prepared to study its inhibition effect on prostate cancer cell PC-3.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Tsai

Chen

2016

IJN

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Green tea is one of the most commonly consumed natural health beverages in Taiwan’s market, with the major functional component catechin being shown to possess several biological activities such as antioxidation, anticancer, and prevention of cardiovascular disease. The objectives of this study were to develop a high-performance liquid chromatography–mass spectrometry method to determine the variety and content of catechins in green tea leaf waste, a by-product obtained during processing of tea beverage. In addition, catechin nanoemulsion was prepared to study its inhibition effect on prostate cancer cell PC-3. Results showed that a total of eight catechin standards were separated within 25 minutes by using a Gemini C18 column and a gradient mobile phase of 0.1% formic acid (A) and acetonitrile (B) with flow rate at 1 mL/min, column temperature at 30°C, and detection wavelength at 280 nm. Among various extraction solvents, 50% ethanol generated the highest yield of total catechins from tea leaf waste, of which five catechins were identified and quantified. The catechin nanoemulsion was composed of catechin extract, lecithin, Tween 80, and deionized water in an appropriate proportion, with the mean particle size being 11.45 nm, encapsulation efficiency 88.1%, and zeta potential −66.3 mV. A high stability of catechin nanoemulsion was shown over a storage period of 120 days at 4°C. Both catechin extract and nanoemulsion could inhibit growth of PC-3 tumor cells, with the half maximal inhibitory concentration being 15.4 μg/mL and 8.5 μg/mL, respectively. The PC-3 cell cycle was arrested at S phase through elevation of P27 expression and decline of cyclin A, cyclin B, cyclin-dependent kinase 2, and cyclin-dependent kinase 1 expression. In addition, both catechin extract and nanoemulsion could induce apoptosis of PC-3 cells through decrease in B-cell lymphoma 2 (bcl-2) expression and increase in cytochrome c expression for activation of caspase-3, caspase-8, and caspase-9. Taken together, both caspase-dependent and caspase-independent pathways may be involved in apoptosis of PC-3 cells.

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Section: Pc-3 Cell Apoptosis Analysismentioning

confidence: 98%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Tsai

Chen

2016

IJN

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“…최근에는 암세포의 세포주기조절을 통해 세포사멸을 유도한다는 연구 가 많이 진행되고 있다 [16,24]. 세포의 성장과 분열에는 G1, S 및 G2/M기로 나눠지는 세포주기가 존재하는데 각 단계에서 의 조절을 통해 세포가 증식할 수 있고, 각각의 단계는 광학 현미경을 통해 관찰 할 수 있다.…”

Section: 서 론unclassified

Silibinin Inhibits Cell Growth and Induces Apoptosis through Cell-cycle Arrest in PC-3 Prostate Cancer Cells

김상헌

김광연²,

유선녕³

et al. 2011

Journal of Life Science

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“…It has also been reported that catechin, a component of green tea and black tea polyphenols, inhibits the tumor growth [12]. Numerous studies support the chemopreventive effect of black tea in the development of lung cancer, prostate cancer, breast cancer, and ovarian cancer [13][14][15][16]. On the basis of these reports we have selected Theaflavins, the major polyphenol of the black tea as experimental compound for our recent study.…”

Section: Introductionmentioning

confidence: 99%

Effects of Theaflavins on Gastric Cancer Cell Line

Roy

Saha

Chatterjee

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to evaluate the anticarcinogenic effects of theaflavins (TFs), component of black tea, on human gastric cancer cell line. Materials and Methods:Cell viability was assessed by WST-1 reagent. Acridine orange (AO) and ethidium bromide (EB) fluorescence staining method were used to detect apoptotic cells, if any, followed by flow cytometric detection. To evaluate the molecular basis for understanding the apoptotic pathway, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were measured. Pro-and anti-apoptotic protein and gene expressions were detected by western blot and real-time techniques.Results: TFs-treated AGS cell indicated apoptosis-mediated cell death. The presence of TFs induced the generation of ROS and which in turn changes the MMP with the elevated level of cyt c, activation of caspase-9, caspase-3, and PARP cleavage. Our study also showed that TFs treatment altered the Bax/Bcl2 ratio and upregulated the expression of p53.Conclusion: All these observations lead to the fact that TFs developed potential chemopreventive effects through the induction of apoptosis through mitochondria-mediated death cascade in the human gastric cancer cell.

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RETRACTED: Theaflavins induce G2/M arrest by modulating expression of p21waf1/cip1, cdc25C and cyclin B in human prostate carcinoma PC-3 cells

Cited by 52 publications

References 40 publications

Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3

Silibinin Inhibits Cell Growth and Induces Apoptosis through Cell-cycle Arrest in PC-3 Prostate Cancer Cells

Effects of Theaflavins on Gastric Cancer Cell Line

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