RETRACTED: RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA

Cui, Yalei; Huang, Tianzhi; Zhang, Xiaobo

doi:10.1098/rsob.150126

Cited by 47 publications

(51 citation statements)

References 42 publications

(82 reference statements)

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“…The findings presented above revealed that the transcription factor Dorsal plays a central role in virus infection. The WSSV genome can encode 89 viral miRNAs (8). The interactions between Dorsal and WSSV miRNAs were investigated to further explore the viral miRNA-mediated regulation of Dorsal gene expression in shrimp.…”

Section: Resultsmentioning

confidence: 99%

Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp

Ren

Huang

Cui

et al. 2017

J Virol

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In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. An miRNA can generally target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spaztle (Spz)-Toll-Dorsal-antilipopolysaccharide factor (ALF) signaling pathway plays a very important role in antiviral immunity against invasion of white spot syndrome virus (WSSV) in shrimp (Marsupenaeus japonicus). Dorsal, the central gene in the Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection. The regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study contributes novel insights into the viral miRNAmediated Toll signaling pathway during the virus-host interaction.IMPORTANCE An miRNA can target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs during virus infection has not yet been extensively explored. The results of this study indicated that the shrimp Dorsal gene, the central gene in the Toll pathway, was targeted by two viral miRNAs during infection with white spot syndrome virus. Regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study provides new insight into the viral miRNA-mediated Toll signaling pathway in virus-host interactions.

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Section: Resultsmentioning

confidence: 99%

Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp

Ren

Huang

Cui

et al. 2017

J Virol

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“…RNA editing occurs by the conversion of adenosine to inosine through the adenosine deaminase acting on RNA (ADAR) family of enzymes [65, 66]. Previous work showed that this process occurs in pri-miRNA transcripts and that miRNA editing causes insufficient Dicer processing [67]. Three ADAR genes, ADAR1, ADAR2, and ADAR3, exist in vertebrates [68-70].…”

Section: Mirna Regulationmentioning

confidence: 99%

The Role of MicroRNAs and Their Targets in Osteoarthritis

2016

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MicroRNA regulation and expression has become an emerging field in determining the mechanisms regulating a variety of inflammation-mediated diseases. Recent studies have focused on specific microRNAs that are differentially expressed in case of osteoarthritis. Furthermore, several targets of these miRNAs important in disease progression have also been identified. In this review, we focus on microRNA biogenesis, regulation, detection, and quantification with an emphasis on cellular localization and how these concepts may be linked to disease processes such as osteoarthritis. Next, we review the relationship of specific microRNAs to certain features and risk factors associated with osteoarthritis such as inflammation, obesity, autophagy, and cartilage homeostasis. We also identify selected microRNAs that are differentially expressed in osteoarthritic tissue, but have unidentified targets and functions in the disease pathogenesis. Lastly, we highlight the potential use of microRNAs for therapeutic purposes, and also point to certain remedies that regulate microRNA expression.

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“…The Co-IP of Sm complex was conducted as described previously with minor modification. 23 The isolated nuclei were suspended in lysis buffer (20 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, pH 7.5). The lysate was treated with RNAsin (Promega, Madison, WI) and then incubated with RQ1 DNAase (Promega) for 5 min at 37°.…”

Section: Co-immunoprecipitation (Coip) Of Sm Complexmentioning

confidence: 99%

Signature microRNAs of nuclear Sm complex associated with breast cancer tumorigenesis

Zhang

Cui

2018

J of Cellular Biochemistry

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It is well known that Sm proteins, small nuclear ribonucleoproteins, act as core spliceosomal factors in alternative splicing of mRNA precursors. MicroRNAs (miRNAs) can function in alternative splicing by targeting mRNAs of splicing factors. However, the direct interaction between miRNAs and proteins of splicing complex in nucleus has not been explored. In this study, the mature miRNAs in nuclear Sm complex of breast cancer cells and normal breast epithelial cells were characterized. Small RNA sequencing of immunoprecipitated nuclear Sm complex with the SmD1-specific antibody identified 123 and 170 mature miRNAs in nuclear Sm complex of normal breast cells and breast cancer cells, respectively. The results of Northern blot analysis confirmed the existence of mature miRNAs in Sm complex and electrophoretic mobility shift assay (EMSA) validated the binding of miRNAs with proteins of Sm complex. Among the identified miRNAs bound to the Sm complex in nucleus, 94 miRNAs were significantly upregulated, and 39 miRNAs significantly downregulated in breast cancer cells compared with normal breast cells, suggesting that miRNAs in nuclear Sm complex might be associated to tumorigenesis of breast cancer by regulating Sm complex during alternative splicing of mRNA precursors. Our study provided novel clues to reveal the regulatory mechanism of Sm complex in the assembly of spliceosome and contributed novel aspects of miRNAs to tumorigenesis of breast cancer.

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RETRACTED: RNA editing of microRNA prevents RNA-induced silencing complex recognition of target mRNA

Cited by 47 publications

References 42 publications

Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp

Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp

The Role of MicroRNAs and Their Targets in Osteoarthritis

Signature microRNAs of nuclear Sm complex associated with breast cancer tumorigenesis

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