RETRACTED: Recent developments in l-glutaminase production and applications – An overview

Binod, Parameswaran; Sindhu, Raveendran; Madhavan, Aravind; Abraham, Amith; Mathew, Anil Kuruvilla; Ummalyma, Sabeela Beevi; Sukumaran, Rajeev; Singh, Sudhir P.; Pandey, Ashok

doi:10.1016/j.biortech.2017.05.059

Cited by 54 publications

(33 citation statements)

References 63 publications

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“…Xylanase [39][40][41] Alkaline protease [42,43] Phytase [44][45][46][47] l-xylosidase [39] Ferulic acid esterase [48] α-amylase [41] Carboxymethyl cellulase [49] Pectinase [41] Laccase [50,51] l-glutaminase [52][53][54][55][56] Milk-clotting enzyme [57] Polygalacturonase (PG) [58] 2.1. Enzymes Production by SSF…”

Section: Ssf Smfmentioning

confidence: 99%

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A Sight to Wheat Bran: High Value-Added Products

et al. 2019

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Recently more consideration has been given to the use of renewable materials and agricultural residues. Wheat production is increasing yearly and correspondingly, the volume of by-products from the wheat process is increasing, as well. It is important to find the use of the residuals for higher value-added products, and not just for the food industry or animal feed purposes as it is happening now. Agricultural residue of the roller milled wheat grain is a wheat bran description. The low-cost of wheat bran and its composition assortment provides a good source of substrate for various enzymes and organic acids production and other biotechnological applications. The main purpose of this review article is to look into recent trends, developments, and applications of wheat bran.

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Section: Ssf Smfmentioning

confidence: 99%

“…The preferable substrate for l-glutaminase manufacturing in SSF was WB [52]. Kashyap et al investigated the glutaminase production from Zygosaccharomyces rouxii NRRL-Y 2547 by SSF using WB and oil cake from sesame [53,55]. El-Sayed reasearched the same enzyme production by Trichoderma koningii using WB as solid support.…”

Section: Ssf Smfmentioning

confidence: 99%

A Sight to Wheat Bran: High Value-Added Products

et al. 2019

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“…In food sector, addition of glutaminase leads to improvement of glutamate content, which enhances pleasant and delicious taste of the foodstuff (Kurihara 2009). The palatable taste of traditional food like soya sauce, miso and sufu is the involvement of L-glutaminase during course of fermentation and leads to accumulation of high content of L-glutamate (Lioe et al 2010;Binod et al 2017). In diagnostic and health monitoring, analysis of L-glutamine and glutamate level in the blood is very important (Madeira et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities

et al. 2020

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Microbial L-glutaminase has considered as one of the most important therapeutic enzymes considering its anticancer or antitumor activity. In this study, one L-glutaminase producing potent fungus was isolated from the coastal soil and identified as Fusarium nelsonii KPJ-2. During parametric optimization, it was noted that wheat bran supported maximum L-glutaminase production than other agro-industrial wastes tested. Solid substrate fermentation was mechanized with optimum pH of 4.0, incubation temperature at 25 °C, inoculums concentration of 2.0% (v/v), substrate concentration of 7.0% (w/v) and moisture of the production media suits at 20.0% (w/v). Statistical optimization using Response Surface Methodology (RSM) was improved the L-glutaminase production by 14.5% (68.93 U/gds) than unoptimized state. The SEM-EDX analysis demonstrated the overgrowth of fungus on wheat bran and utilization of its associated minerals. A comparative cytotoxic effect of the partial purified glutaminase was examined on both cancerous HCT cell and normal Vero cell line. The result clearly demonstrated that L-glutaminase from F. nelsonii KPJ-2 is specifically cytotoxic against cancer cell line with IC50 of 203.95µg/ml, but, non-responsive against normal cell. The newly isolated fungal strain can produce a considerable amount of L-glutaminase utilizing very low-cost substrate and the enzyme have therapeutic value for real life application owing to its anticancer effectiveness.

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“…L-glutaminase's promising therapeutic alternative to the treatment of leukaemia and retroviral diseases justifies this research attention (Binod et al, 2017). L-glutaminase's applications span across the food and medical industries.…”

Section: Introductionmentioning

confidence: 99%

“…Several microorganisms have been reported to secrete L-glutaminase into fermentation media. Among the studied organisms, fungi are reported as the most potent producers of the enzyme (Binod et al, 2017). Several species, from the genus of Penicillium (El-Shafei et al, 2014) Saccharomyces (Iyer and Rekha, 2010) and Aspergillus (Dutt et al, 2014)…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of Lglutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

Olarewaju¹,

Nzelibe²

2019

Afr. J. Biochem. Res.

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Six strains of Trichoderma species isolated from soil and air samples were screened for extracellular Lglutaminase production. The highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. A specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine, 0.5% (w/v) glucose, 1% (w/v) NaCl, pH 3.0 and 1.4 ml inoculums size respectively after 3 days of incubation at 27°C as the optimum condition for L-glutaminase production. Asides glutamine, other nitrogen sources; malt extract and ammonium sulphate had a negative influence on enzyme production likewise the supplemented sources of metal ions; zinc and iron (II) ions.

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RETRACTED: Recent developments in l-glutaminase production and applications – An overview

Cited by 54 publications

References 63 publications

A Sight to Wheat Bran: High Value-Added Products

A Sight to Wheat Bran: High Value-Added Products

A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities

Statistical optimization of Lglutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

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