RETRACTED: Production of transgenic orchardgrass via Agrobacterium-mediated transformation of seed-derived callus tissues

Lee, Sanghoon; Lee, Dong-Gi; Woo, Hyun-Sook; Lee, Ki-Won; Kim, Do‐Hyun; Kwak, Sang‐Soo; Kim, Jin-Seog; Kim, Hyegi; Ahsan, Nagib; Choi, Myung Suk; Yang, Jae-Kyung; Lee, Byung-Hyun

doi:10.1016/j.plantsci.2006.05.006

Cited by 36 publications

(25 citation statements)

References 33 publications

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“…Kinetin and zeatin were used to induce callus capable of bud formation [25]. Benzyladenine increased the resistance of plants against environmental stresses, for example it increased RuBPCase activity of wheat seedling under water deficit [39].…”

Section: Introductionmentioning

confidence: 99%

Plant growth regulators induced urease activity inCucurbita pepoL. cotyledons

Shora

Ali

2016

Acta Biologica Hungarica

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This study is aimed to investigate the activity of urease (EC 3.5.1.5, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old Cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. The treatment of C. pepo cotyledons with different concentrations (100-600 µmol) of different auxins [indole-3-acetic acid (IAA), indole butyric acid (IBA), indole propionic acid (IPA) and naphthalene acetic acid (NAA)]; or with different concentrations (100-300 µmol) of different cytokinins [kinetin, zeatin and benzyladenine (6-BA)] resulted in a significant increase of urease activity, compared to control. The optimal effects were recorded for each of 500 µmol of IAA and 300 µmol of zeatin treatments. A gradual increase in urease activity was detected in cotyledons treated with various concentrations (0.2-1.0 mM) of 28-homobrassinolide (HBL), in relative to control. A substantial increase in urease activity was observed in cotyledons subjected to different concentrations of triazole (10-60 mg L -1 ), containing either triadimefon (TDM) or hexaconazole (HEX), compared to control. The combination of 300 µmol zeatin with any of protein inhibitors, namely 5-fluorouridine (FUrd), cordycepin and α-amanitin, resulted in the alleviation of their inhibitory effect on the urease activity.

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Section: Introductionmentioning

confidence: 99%

Plant growth regulators induced urease activity inCucurbita pepoL. cotyledons

Shora

Ali

2016

Acta Biologica Hungarica

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“…Genomic DNA was isolated from the leaf tissues of putative transgenic (T0) and wild-type plants, as described previously [14]. Internal fragments of CP4-EPSPS and bar were amplified by two individual PCRs.…”

Section: Molecular Analyses Of Transgenic Plantsmentioning

confidence: 99%

“…Genomic DNA (20 mg) was digested with HindIII and separated by electrophoresis in a 1.2% agarose gel, then transferred to a Hybond Nþ (Amersham, Buckinghamshire, UK) membrane. The earlier CP4-EPSPS-specific PCR product was used as a probe and Southern blot hybridization was carried out as described previously [14]. …”

Section: Molecular Analyses Of Transgenic Plantsmentioning

confidence: 99%

Development of antibiotic marker-free creeping bentgrass resistance against herbicides

Lee¹,

Kim²,

Kim³

et al. 2011

ABBS

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Herbicide-resistant creeping bentgrass plants (Agrostis stolonifera L.) without antibiotic-resistant markers were produced by Agrobacterium-mediated transformation. Embryogenic callus tissues were infected with Agrobacterium tumefaciens EHA105, harboring the bar and the CP4-EPSPS genes for bialaphos and glyphosate resistance. Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained at 14-16 weeks after transformation. Genetic transformation of the selected, regenerated plants was validated by PCR. Southern blot analysis revealed that at least one copy of the transgene was integrated into the genome of the transgenic plants. Transgene expression was confirmed by Northern blot. CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta, containing 0.5% glufosinate ammonium or glyphosate. The optimized Agrobacteriummediated transformation method resulted in an average of 9.4% transgenic plants. The results of the present study suggest that the optimized marker-free technique could be used as an effective and reliable method for routine transformation, which may facilitate the development of varieties of new antibiotic-free grass species.

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“…Nowadays, mature seeds are considered an important source of explants, and are extensively used for plant regeneration via callus culture (Lee et al, 2006(Lee et al, , 2008. Therefore, we made an attempt to develop an efficient regeneration system from mature seed-derived callus of Dahurian wildrye grass for genetic transformation.…”

Section: Introductionmentioning

confidence: 99%