RETRACTED: Physcion 8-O-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial to mesenchymal transition of hepatocellular carcinoma HepG2 cells

Wang, Qiang; Wang, Yong; Xing, Yuqing; Yan, Yongyong; Guo, Peng; Zhuang, Jianguang; Qin, Fawei; Zhang, Jie

doi:10.1016/j.biopha.2016.06.045

Cited by 20 publications

(18 citation statements)

References 38 publications

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“…Physcion 8-O-b-glucopyranoside (PG) is the most active ingredient of this plant and has been shown to display anti-tumour and anti-inflammatory properties. It is effective against various cancers, including breast cancer [6], glioblastoma [7], hepatocellular carcinoma [8], oral squamous cell carcinoma [9], and osteosarcoma [10]. Moreover, PG extracted from Polygonum cuspidatum is shown to exert anti-proliferative and anti-inflammatory impacts on MH7A rheumatoid arthritis-derived fibroblast-like synoviocytes [11].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ATICLE: Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling

Wan

Long

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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2020) Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/ STAT3 signalling, Artificial ABSTRACT Diabetic retinopathy (DR) is the leading cause of decreased vision and blindness globally. The aim of this study was to understand the role of physcion 8-O-b-glucopyranoside (PG) in high glucose (HG)induced DR and to investigate whether lncRNA NORAD/miR-125/STAT3 signalling was the underlying mechanism involved in DR. To this end, the serum levels of NORAD, miR-125, and STAT3 were determined in patients with DR. The APRE-19 cells were subjected to HG treatment to construct the cell model of DR. HG-disposed APRE-19 cell injury was assessed by detecting cell viability, apoptosis, concentrations of pro-inflammatory cytokines including TNF-a and IL-1b, and ROS generation. Moreover, the effect of PG on HG-disposed APRE-19 cell injury was investigated. NORAD was then overexpressed to investigate the combined effects of NORAD overexpression and PG on HG-disposed APRE-19 cell injury. Furthermore, the regulatory relationship between NORAD and miR-125 as well as miR-125 and STAT3 was investigated. The expression levels of NORAD and STAT3 were significantly increased in the serum of DR patients, while the miR-125 expression was decreased. The HG treatment-induced injury to APRE-19 cells, which were alleviated by PG treatment. Moreover, PG alleviated HG-disposed injury to ARPE-19 cells by decreasing NORAD. NORAD negatively regulated miR-125 expression and the combined effects of NORAD and PG on HG-disposed ARPE-19 cell injury were reversed by miR-125 overexpression. Furthermore, STAT3 was confirmed as a target gene of miR-125. Our results show that PG exerts protective roles in HG-disposed DR via regulating lncRNA NORAD/miR-125/STAT3 signalling. NORAD/miR-125/STAT3 axis may provide a novel perspective for target therapy of DR. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

RETRACTED ATICLE: Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling

Wan

Long

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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“…Physcion 8-O-b-glucopyranoside (PG) is one of main active ingredients. PG has been pointed out to exert anti-tumour effects in diverse cancers, such as hepatocellular carcinoma [23], melanoma [24], breast cancer [25], oral squamous cell carcinoma [26] and clear-cell renal cell carcinoma [27]. However, the effect of PG on ovarian cancer development has not been clarified.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Involvement of NORAD/miR-608/STAT3 axis in carcinostasis effects of physcion 8-O-β-glucopyranoside on ovarian cancer cells

Yang

Yan

Chen

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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We planned to dig the carcinostasis activity of physcion 8-O-b-glucopyranoside (PG) in ovarian cancer cells and explored whether long non-coding RNA NORAD was the potential cause of the carcinostasis impact of PG. The impacts of PG on the tumour cell behaviours (including cell viability, apoptosis, migration and invasion) of SKOV3 cells were grabbed. The levels of NORAD in cancer tissues and cell lines were determined; afterwards, the impacts of abnormal expression of NORAD on the tumour cell behaviours of SKOV3 cells were assessed. Moreover, we explored whether NORAD modulated the level of STAT3 by competitively sponging miR-608, thus mediating the antineoplastic effects of PG on ovarian cancer cells. PG suppressed cell viability, enhanced apoptosis and lessened migration and invasion of SKOV3 cells. NORAD was upregulated in ovarian cancer tissues and cells. Silencing of NORAD lessened cell viability, migration and invasion, but induced apoptosis of SKOV3 cells, whereas overexpression of NORAD had opposite effects. Moreover, PG decreased the expression of NORAD. Overexpression of NORAD reversed the effects of PG treatment on the cell biological performances of SKOV3 cells, which were further reversed after overexpression of miR-608 simultaneously. Furthermore, STAT3 was tested as a target gene of miR-608, and the impact of NORAD in PG-treated SKOV3 cells were through the miR-608-mediated STAT3. Our findings reveal that NORAD/miR-608/STAT3 axis is pivotal in mediating the antineoplastic impacts of PG on ovarian cancer cells, which may offer a novel explanation in the therapy of ovarian cancer.

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“…Some studies have demonstrated the cytotoxic effects of physcion, dependent on the activation of caspases, and cell cycle arrest in HeLa (cervical cancer cells), SW620 (colorectal cancer cells), and MDA-MB-231 (breast cancer cells) [ 53 – 55 ]. Studies using physcion-8-O- β glucopyranoside have also shown significant cytotoxic activity in HepG2, A549, and HEM (human melanoma cells) [ 56 – 58 ].…”

Section: Resultsmentioning

confidence: 99%

“…Studies have associated the toxic effect of emodin and physcion with an increase in intracellular RONS, which thereby promotes deregulation of mitochondrial membrane potential and activation of cell death [ 50 , 52 , 53 , 56 ]. Therefore, the levels of thiobarbituric acid reactive species (TBARS) were quantified after the treatment of the cells with emodin, physcion, and EERs.…”

Section: Resultsmentioning

confidence: 99%

Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines

Moreira

Sorbo

Souza

et al. 2018

Oxidative Medicine and Cellular Longevity

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There have been few studies on the pharmacological properties of Rhamnus sphaerosperma var. pubescens, a native Brazilian species popularly known as “fruto-de-pombo.” The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO• and O2•−, while all samples were equally effective in HOCl/OCl− capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC-3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Emodin decreased the activation of AKT in all tumor cells, physcion in HSC-3 and HaCaT cells, and the ethanolic extract in C33A and HaCaT cells, respectively. The induction of cancer cell death by emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens was related to an increase in intracellular oxidative stress and DNA damage and a decrease in AKT activation. These molecules are therefore emerging as interesting candidates for further study as novel options to treat cervical and oral carcinomas.

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RETRACTED: Physcion 8-O-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial to mesenchymal transition of hepatocellular carcinoma HepG2 cells

Cited by 20 publications

References 38 publications

RETRACTED ATICLE: Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling

RETRACTED ATICLE: Physcion 8-O-β-glucopyranoside exerts protective roles in high glucose-induced diabetic retinopathy via regulating lncRNA NORAD/miR-125/STAT3 signalling

RETRACTED ARTICLE: Involvement of NORAD/miR-608/STAT3 axis in carcinostasis effects of physcion 8-O-β-glucopyranoside on ovarian cancer cells

Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines

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