2021
DOI: 10.17504/protocols.io.brhum36w
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[RETRACTED] Multiplexed scNOMe-seq protocol based on isolated single nuclei v1

Abstract: What follows is the protocol for performing single-cell Nucleosome Occupancy and Methylome sequencing on single nuclei (scNOMe-seq). This plate-based method, which can be used to simultaneously assess nucleosome occupancy and DNA methylation in single nuclei, draws upon the approach laid out in Luo et al., 2018. Everything from the initial nuclei sorting step to the final library preparation that directly precedes sequencing is included in this protocol. This protocol is optimized for non-neuronal nuclei and c… Show more

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