RETRACTED: miR-361-5p inhibits hepatocellular carcinoma cell proliferation and invasion by targeting VEGFA

Cui, Wenxian; Li, Yuanguo; Xu, Keqing; Chen, Guijin; Lu, Xiaoqun; Duan, Qiuqin; Kang, Zhirong

doi:10.1016/j.bbrc.2016.09.076

Cited by 24 publications

(10 citation statements)

References 17 publications

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“…HCC cells commonly become resistant to DDP, resulting in chemotherapy failure (38). The present data and those of previous reports demonstrated that miR-361-5p significantly inhibited the proliferation and induced apoptosis of HCC cells (19,20). However, only the ratio of cleaved caspase-3 and caspase-3 (apoptosis-related proteins) were used to measure cell apoptosis in the present study.…”

Section: Discussionmentioning

confidence: 50%

“…A previous study has demonstrated that miR-3174 is highly expressed in HCC (17). miR-361-5p has been reported to be downregulated in HCC (20) and Sun et al (19) reported that miR-361-5p inhibits HCC cell growth. However, the function of miR-361-5p in the chemoresistance of HCC and the potential molecular mechanism of action remain unknown.…”

Section: Introductionmentioning

confidence: 98%

“…miRNAs participate in a number of biological processes and changes in the expression and function of miRNAs are implicated in several diseases (16). Accumulating evidence has demonstrated that multiple abnormally expressed miRNAs are associated with the development of HCC, including miR-3174, miR-383, miR-361-5p and miR-330-5p (17)(18)(19)(20)(21). Wang et al (18) demonstrated that miR-383 suppresses cell proliferation and induces apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA‑361‑5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9

et al. 2021

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MicroRNAs (miRs) participate in the development of several cancers. miR-361-5p suppresses the proliferation of hepatocellular carcinoma (HCC) cells. However, its function and potential underlying mechanism of action in the chemoresistance of HCC remains unknown. Therefore, cisplatin (DDP)-resistant HCC cells were used to study the role and potential mechanism of action of miR-361-5p in HCC resistance to chemotherapy. TargetScan software and dual-luciferase reporter assays were used to determine whether MAPK kinase kinase 9 (MAP3K9) is a target gene of miR-361-5p. Subsequently, reverse transcription-quantitative PCR and western blot analyses demonstrated that miR-361-5p mimic decreased MAP3K9 expression levels in Huh7 cells and this change was reversed by transfection with the MAP3K9-plasmid. In addition, compared with THLE-2 cells, miR-361-5p was downregulated, while MAP3K9 was upregulated in Huh7 cells. MAP3K9 also reversed the miR-361-5p-induced HCC cell apoptosis. A DDP-resistant cell line, Huh7/DDP, was established and MTT analysis revealed that the IC 50 value of DDP treatment in Huh7/DDP cells was higher compared with that in Huh7 cells. miR-361-5p expression was lower in Huh7/DDP cells compared with that in Huh7 cells. Similarly, miR-361-5p downregulated the expression levels of MAP3K9 in Huh7/DDP cells. Furthermore, MAP3K9 reversed miR-361-5p-induced sensitivity of Huh7/DDP cells to DDP and miR-361-5p induced Huh7/DDP cell apoptosis. Therefore, the findings of the present study demonstrated that the miR-361-5p/MAP3K9 axis may serve as a new potential biomarker and therapeutic target for DDP-resistant HCC.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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MicroRNA‑361‑5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9

et al. 2021

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“…Subsequently, we performed a search in PubMed for these 14 miRNAs and found that 7 of them had been confirmed experimentally to target VEGFA, that is, miR-361 [40, 41], miR-140 [42], miR-15a [43], miR-15b [44], miR-205 [45], miR-206 [46], and miR-200c [47]. Therefore, we speculate that these miRNAs may target VEGFA and play a role in NSC behavior under hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Identification and Verification of Candidate Genes Regulating Neural Stem Cells Behavior Under Hypoxia

Shi

Wei

et al. 2018

Cell Physiol Biochem

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Background/Aims: Neural stem cells (NSCs) reside in a hypoxic environment, and hypoxia plays an important role in their development and differentiation. This study aimed to explore the underlying mechanisms by which hypoxia affects NSC behavior. Methods: In the current study, we downloaded the gene expression dataset GSE68572 and identified the differentially expressed genes (DEGs) by analyzing high-throughput gene expression in hypoxic and normoxic NSCs. Subsequently, we analyzed these data using a combined bioinformatics approach and predicted the microRNAs (miRNAs) targeting the key gene using miRNA databases. Quantitative real-time PCR (qRT-PCR) was used to validate the expression of the top five DEGs. Results: In total, 1347 genes were identified as DEGs. We identified the predominant gene ontology categories and Kyoto Encyclopedia of Genes and Genomes pathways that were significantly over-represented in the hypoxic NSCs. A protein–protein interaction network he identification of miRNAs and their putative targets may offer new diagnostic and therapeutic strategies for liver cancer the top 10 core genes. Vascular endothelial growth factor A (VEGFA) had the highest degree and may be the key gene concerning NSC behavior under hypoxia. Further validation of the top five DEGs by qRT-PCR demonstrated that four DEGs were significantly higher and one DEG was significantly lower in the hypoxic group than in the control group. Seven miRNAs were predicted and proved to target VEGFA. Conclusion: This preliminary study can prompt the understanding of the molecular mechanisms by which hypoxia has an impact on NSC behavior and can help to optimize stem cell therapies for central nervous system injuries and diseases.

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“…Furthermore, miR-663 has been demonstrated to promote cell proliferation, migration and invasion by targeting TUSC2 in ovarian cancer (15). Cui et al (16) reported that miR-361-5p was downregulated in hepatocellular carcinoma (HCC) and suppressed HCC proliferation and invasion through targeting VEGFA. Ma et al (17) demonstrated that miR-361-5p expression was associated with prognosis in patients with breast cancer.…”

Section: Introductionmentioning

confidence: 99%

miR‑361‑5p regulates ovarian cancer cell proliferation and apoptosis by targeting TRAF3

Ling

2021

Exp Ther Med

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An increasing body of evidence has demonstrated that the abnormal expression of microRNAs (miRNAs) participate in the development and progression of ovarian cancer. miR-361-5p has been reported to serve as a tumor suppressor or oncogene in a number of different human cancer types. In the current study, it was indicated that miR-361-5p was highly expressed in ovarian cancer tissues. Compared with human ovarian epithelial cells HOSEpiC, miR-361-5p was upregulated in ovarian cancer cell lines, including in ES-2 and SKOV3 cells. The binding sites between TNF receptor-associated factor 3 (TRAF3; a member of the TRAF family of cytoplasmic adaptor proteins) and miR-361-5p were predicted using TargetScan, and a dual luciferase reporter gene assay verified the result. Subsequently, a reverse transcription-quantitative PCR assay and western blot assay indicated that TRAF3 was downregulated in ovarian cancer tissues and cell lines. It was demonstrated that miR-361-5p inhibitor significantly reduced the viability of SKOV3 cells and induced apoptosis. However, all changes were reversed by TRAF3 silencing. Furthermore, it was demonstrated that miR-361-5p inhibitor decreased the expression of p-p65 in SKOV3 cells, indicating the inhibition of the NF-kB signaling pathway. In conclusion, miR-361-5p may regulate the proliferation and apoptosis of ovarian cancer cells by targeting TRAF3. Therefore, targeting miR-361-5p may exhibit therapeutic potential in the treatment of ovarian cancer.

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RETRACTED: miR-361-5p inhibits hepatocellular carcinoma cell proliferation and invasion by targeting VEGFA

Cited by 24 publications

References 17 publications

MicroRNA‑361‑5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9

MicroRNA‑361‑5p induces hepatocellular carcinoma cell apoptosis and enhances drug sensitivity by targeting MAP3K9

Identification and Verification of Candidate Genes Regulating Neural Stem Cells Behavior Under Hypoxia

miR‑361‑5p regulates ovarian cancer cell proliferation and apoptosis by targeting TRAF3

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