RETRACTED: MiR-19-3p Induces Tumor Cell Apoptosis via Targeting FAS in Rectal Cancer Cells

Su, Yinan; Zang, Yifeng; Wang, Yuhong; Ding, Youming

doi:10.1177/1533033820917978

Cited by 12 publications

(10 citation statements)

References 19 publications

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“…Our results revealed that miR-19a-3p inhibited cell proliferation, migration, and invasion in U226 and RPMI-8226 cells. This agreed with previous reports showing that overexpression of miRNA-19a-3p significantly reduced the cell proliferation, migration, and invasion ability of HCT116 human colon cancer cells ( 26 ). To investigate the mechanisms of miRNA-19a-3p-mediated inhibition of cell proliferation, migration, and invasion, bioinformatics software was used to predict the target genes of miRNA-19a-3p.…”

Section: Discussionsupporting

confidence: 93%

The microRNA miR-19a-3p suppresses cell growth, migration, and invasion in multiple myeloma via the Wnt/β-catenin pathway

Wei¹,

Wang²,

Li³

et al. 2021

Transl Cancer Res TCR

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Background MicroRNAs have been suggested as potential regulators in the development of multiple myeloma (MM) through affecting the expression of their target genes. This study aimed to investigate the effects of miR-19a-3p in MM, and its underlying mechanisms in regulating cell proliferation and invasion. Methods Bone marrow samples from 25 MM patients and 12 healthy donors were collected and miR-19a-3p and Wnt1 mRNA expression was assessed. The effects of miR-19a-3p on cell proliferation, migration, and invasion in U226 and RPMI-8226 MM cells were evaluated by miR-19a-3p overexpression. Luciferase assays were performed to explore the potential target genes. Knock down or overexpression of Wnt1 was used to explore the effects of miR-19a-3p on cell growth, migration, and invasion. Results The expression of miR-19a-3p was downregulated in MM and cell lines, while Wnt1 mRNA levels were increased. Overexpression of miR-19a-3p inhibited cell proliferation, migration, and invasion in U226 and RPMI-8226 cells. Additionally, western blot assays revealed that miR-19a-3p could suppress Wnt1, β-catenin, cyclin D1, and c-Myc expression. Knockdown of Wnt1 also inhibited cell growth, migration, and invasion. Moreover, luciferase reporter assay revealed direct binding between Wnt1 and miR-19a-3p. Wnt1 overexpression partially reversed the suppressive effects of miR-19a-3p on cell proliferation, migration, and invasion in U266 cells. Conclusions The expression of miR-19a-3p was downregulated in MM patients and MM cell lines. Overexpression of miR-19a-3p inhibited proliferation, migration, and invasion by targeting Wnt1 via the Wnt/β-catenin signaling pathway.

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Section: Discussionsupporting

confidence: 93%

The microRNA miR-19a-3p suppresses cell growth, migration, and invasion in multiple myeloma via the Wnt/β-catenin pathway

Wei¹,

Wang²,

Li³

et al. 2021

Transl Cancer Res TCR

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“…Both miR-19a-3p and miR-19a-5p have been reported to be crucial in the promotion or inhibition of cancer progression. For example, miR-19a-3p was reported to promote the proliferation of hepatocellular carcinoma cells via regulation of the PIK3IP1/AKT pathway ( 30 ); however, another study reported that miR-19-3p induces colorectal cancer cell apoptosis by repressing the expression of Fas cell surface death receptor ( 31 ). Furthermore, miR-19a-5p expression is significantly decreased in NSCLC, and miR-19a-5p can suppress the proliferation, migration and invasion of NSCLC cells ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

Wang

Zhang

et al. 2021

Oncol Lett

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GATA6 antisense RNA 1 (GATA6-AS1) has been reported to be involved in the progression of several types of cancer. In the present study, the role of GATA6-AS1 in ovarian cancer (OC) was explored. Reverse transcription quantitative PCR was used to detect the expression of GATA6-AS1, microRNA (miR)-19a-5p and tet methylcytosine dioxygenase 2 (TET2) in OC and adjacent normal tissues. Furthermore, OC cells with GATA-AS1 either knocked down or overexpressed were established. The Cell Counting Kit-8 assay was used to evaluate cell proliferation and a Transwell assay was used to assess the migratory and invasive abilities of OC cells. A dual luciferase reporter gene assay was used to determine whether GATA6-AS1 and miR-19a-5p, and miR-19a-5p and TET2, may interact with each other. The results demonstrated that GATA6-AS1 expression level was decreased in OC tissues and cells compared with control groups. In addition, GATA6-AS1 overexpression significantly inhibited the proliferation and migratory and invasive abilities of OC cells, whereas GATA6-AS1 downregulation had the opposite effects. Furthermore, GATA6-AS1 adsorbed miR-19a-5p to repress its expression and GTA6-AS1 indirectly upregulated TET2 expression. Taken together, the findings from this study suggested that GATA6-AS1 could inhibit the proliferation and migratory and invasive abilities of OC cells via regulation of the miR-19a-5p/TET2 axis.

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“…miRNAs function as important endogenous regulators of gene expression and are thereby implicated in modulating various biological processes through complementary miRNA-mRNA binding to form silencing complexes (25,(29)(30)(31)(32)(33)(34)(35). Recent evidence has indicated that the dysregulation of miRNA is involved in numerous pathological processes (36)(37)(38), and miRNA inhibitors have been demonstrated to have tremendous therapeutic potential in a multitude of studies (39,40). Therefore, continuous exploration of the roles of miRNAs will provide possible therapeutic targets for MOVC.…”

Section: Discussionmentioning

confidence: 99%

miR‑3133 inhibits proliferation and angiogenesis by targeting the JUNB/VEGF pathway in human umbilical vein endothelial cells

Cao

Zhang

et al. 2020

Oncol Rep

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Membranous obstruction of the inferior vena cava (MOVC) has the highest incidence rate among the different types of Budd-Chiari syndrome (BCS) in China. The inferior vena cava septum of patients with MOVC contains capillaries and the two surfaces of the membrane are composed of vascular endothelial tissue. Membrane formation occurs due to endothelial damage. MicroRNAs (miRNAs/miRs) have been verified to be involved in the pathogenesis and progression of various human diseases. A previous study by our group suggested that miR-3133 was downregulated in the serum of patients with MOVC. In the present study, the possible mechanistic implication of miR-3133 in MOVC-associated processes was further explored. It was observed that miR-3133 overexpression inhibited, whereas miR-3133 knockdown enhanced the proliferation and tube formation of human umbilical vein endothelial cells (HUVECs) using the CCK-8 and tube formation assays. JUNB, a member of activator protein 1 and an important upstream transcriptional molecule of vascular endothelial growth factor (VEGF), was proven to be a direct target gene of miR-3133 using a bioinformatics prediction and luciferase reporter assay. Meanwhile, the mRNA and protein expression of JUNB and VEGF was determined by PCR, ELISA and western blot analyses. Of note, miR-3133 overexpression downregulated, while miR-3133 knockdown elevated the expression of JUNB and VEGF significantly. Furthermore, it was demonstrated that JUNB upregulated the expression and secretion of VEGF to promote HUVEC proliferation and angiogenesis. miR-3133 was able to inhibit the effect of JUNB overexpression to promote cell proliferation, angiogenesis and the expression of VEGF. In conclusion, the present study demonstrated that miR-3133 regulated endothelial cell proliferation and angiogenesis through the JUNB/VEGF pathway, which may provide an approach for inhibiting diaphragm formation of the inferior vena cava in MOVC.

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RETRACTED: MiR-19-3p Induces Tumor Cell Apoptosis via Targeting FAS in Rectal Cancer Cells

Cited by 12 publications

References 19 publications

The microRNA miR-19a-3p suppresses cell growth, migration, and invasion in multiple myeloma via the Wnt/β-catenin pathway

The microRNA miR-19a-3p suppresses cell growth, migration, and invasion in multiple myeloma via the Wnt/β-catenin pathway

GATA6‑AS1 inhibits ovarian cancer cell proliferation and migratory and invasive abilities by sponging miR‑19a‑5p and upregulating TET2

miR‑3133 inhibits proliferation and angiogenesis by targeting the JUNB/VEGF pathway in human umbilical vein endothelial cells

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