[Retracted] MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation

Yao, Xiang‐Dong; Li, Ping; Wang, Ji‐Sheng

doi:10.3892/etm.2021.9977

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…MiR-125a-5p was significantly differentially expressed between tumor tissues and normal tissues and was related to the occurrence, growth, and invasion of LSCC, so it may represent a new biomarker for biotherapy [ 15 ]. Our research corroborates these conclusions and reveals the mechanisms of actionof miR-125a-5p in laryngeal carcinoma for the first time.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

et al. 2023

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Background MiRNAs can affect the radiosensitization of head and neck squamous cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect on radiation in laryngeal squamous cell cancer (LSCC). Methods First, we systematically analyzed the role of the miR-125 family in HNSCC using the TCGA database and found that miR-125a-5p is associated with radiotherapy. We then performed comprehensive enrichment analysis of miR-125a-5p and predicted target genes. Then, we performed transfection, cell proliferation assays, reverse transcription polymerase chain reaction, apoptosis assays, micronucleus tests, and western blotting on hep-2 cells selected with puromycin. Results MiR-125 family members exhibited significantly different expression in HNSCC. They were significantly associated with tumor–node–metastasis staging, clinical stages, and histological grades. Radiation therapy had a statistically effect on miR-125 family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of miR-125a-5p. The proliferation rate of cells transfected with lentivirus vector expressing miR-125a-5p was significantly reduced compared to the other groups. The radiation effect was enhanced in cells transfected with miR-125a-5p. The ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly higher than that of the Ad-control group. Western blotting analysis revealed that miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes. Conclusions MiR-125 family members could be prognostic biomarkers of HNSCC and improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the effect of radiotherapy on LSCC.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

et al. 2023

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“…Therefore, we further analyzed the correlation between miR-125a-5p and LSCC sensitivity to radiotherapy. MiR-125a-5p was signi cantly differentially expressed between tumor tissues and normal tissues and was related to the occurrence, growth, and invasion of LSCC, so it may represent a new biomarker for biotherapy [15]. Our research corroborates these conclusions and reveals the mechanisms of actionof miR-125a-5p in laryngeal carcinoma for the rst time.…”

Section: Discussionsupporting

confidence: 83%

MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

Wang

Liu

Tan

et al. 2023

Preprint

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Backgroud: MiRNAs can affect the radiosensitization of head and neck squamous cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect on radiation in laryngeal squamous cell cancer (LSCC). Methods: First, we systematically analyzed the role of the miR-125 family in HNSCC using the TCGA database and found that miR-125a-5p is associated with radiotherapy. We then performed comprehensive enrichment analysis of miR-125a-5p and predicted target genes. Then, we performed transfection, cell proliferation assays, reverse transcription polymerase chain reaction, apoptosis assays, micronucleus tests, and western blotting on hep-2 cells selected with puromycin. Results: MiR-125 family members exhibited significantly different expression in HNSCC. They were significantly associated with tumor–node–metastasis staging, clinical stages, and histological grades. Radiation therapy had a statistically effect on miR-125 family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of miR-125a-5p. The proliferation rate of cells transfected with lentivirus vector expressing miR-125a-5p was significantly reduced compared to the other groups. The radiation effect was enhanced in cells transfected with miR-125a-5p. The ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly higher than that of the Ad-control group. Western blotting analysis revealed that miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes. Conclusions: MiR-125 family members could be prognostic biomarkers of HNSCC and improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the effect of radiotherapy on LSCC.

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Up-regulation of exosomal miR-125a in pneumoconiosis inhibits lung cancer development by suppressing expressions of EZH2 and hnRNPK

Zhang

Hao

et al. 2018

RSC Adv.

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[Retracted] MicroRNA differential expression spectrum and microRNA‑125a‑5p inhibition of laryngeal cancer cell proliferation

Cited by 8 publications

References 20 publications

RETRACTED ARTICLE: MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

RETRACTED ARTICLE: MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma

Up-regulation of exosomal miR-125a in pneumoconiosis inhibits lung cancer development by suppressing expressions of EZH2 and hnRNPK

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