RETRACTED: Long Non-coding RNA LINC00320 Inhibits Tumorigenicity of Glioma Cells and Angiogenesis Through Downregulation of NFKB1-Mediated AQP9

Abstract

The inhibitory effect of long intergenic non-coding RNA 00320 (LINC00320) in glioma cell proliferation has been proposed in a recent study. However, the mechanisms by which LINC00320 regulate aquaporin 9 (AQP9) in glioma require further exploration. Hence, this study aims to investigate effects of LINC00320 on tumorigenicity of glioma cells and angiogenesis of microvascular endothelial cells (MVECs). Expression of LINC00320 and AQP9 in glioma tissues and cells was measured by reverse transcription–quantitative polymerase chain reaction and Western blot analysis. The relationship among LINC00320, nuclear factor κB subunit 1 (NFKB1) and AQP9 was examined by RNA immunoprecipitation, dual-luciferase reporter gene, and chromatin immunoprecipitation assays. The participation of LINC00320 and AQP9 in glioma cell proliferation and MVEC angiogenesis was analyzed using gain- and loss-of-function approaches. Finally, a nude mouse orthotopic xenograft model of glioma was established to investigate the effects of LINC00320 and AQP9 on glioma growth in vivo. LINC00320 was under-expressed and AQP9 was over-expressed in glioma tissues. Further mechanistic investigation showed that LINC00320 downregulated AQP9 by inhibiting the recruitment of NFKB1 to the promoter region of AQP9. LINC00320 overexpression or AQP9 silencing inhibited the proliferation of glioma cells and angiogenesis of MVECs. Also, upregulation of LINC00320 restrained tumor growth and angiogenesis in xenograft mice by downregulating AQP9. Taken together, LINC00320 acts as a tumor suppressor in glioma, thus presenting a novel therapeutic target.

A novel risk score model based on five angiogenesis-related long non-coding RNAs for bladder urothelial carcinoma

“…LncRNA PAXIP1-AS1 was identified to boost migration and angiogenesis of glioma by upregulating ETS1-midiated KIF14 expression [ 36 ]. Aside from the pro-angiogenetic potential, Chang et al found lncRNA LINC00320 suppressed tumourigenicity of glioma and angiogenesis through reduction of NFKB1-regulated AQP9 [ 37 ]. A growing body of ARlncRs not only provided therapeutic targets, but were also promising for diagnosis and prognosis evaluation.…”

A novel risk score model based on five angiogenesis-related long non-coding RNAs for bladder urothelial carcinoma

“…LncRNA PAXIP1-AS1 was identi ed to boost migration and angiogenesis of glioma by upregulating ETS1midiated KIF14 expression 36 . Aside from the pro-angiogenetic potential, Chang et al found lncRNA LINC00320 suppressed tumourigenicity of glioma and angiogenesis through reduction of NFKB1-regulated AQP9 37 . A growing body of ARlncRs not only provided therapeutic targets, but were also promising for diagnosis and prognosis evaluation.…”

The role of long non-coding RNAs in angiogenesis and anti-angiogenic therapy resistance in cancer

“… 96 Analysis of database GSE104291 indicated that there were 91 genes with high expression and 268 genes with low expression. 73 lncRNA SLC26A4-AS1 was meaningly downregulated in glioma tissues and cells. Upregulation of SLC26A4-AS1 in U251 and SHG44 cells inhibited the proliferation, migration, and angiogenesis of U251 and SHG44 cells.…”

“… 72 However, LINC00320 inhibited AQP9 expression by recruiting NFKB1 to the promoter region of AQP9, thus acting as a tumor suppressor gene in glioma ( Figure 2 F). 73 Osteosarcoma is a common and highly malignant bone tumor, mostly occurring in adolescents. lncRNA TUG1 was overexpressed in osteosarcoma tissues and is associated with poor prognosis of patients.…”