RETRACTED: lncRNA SPRY4-IT1 Regulates Cell Proliferation and Migration by Sponging miR-101-3p and Regulating AMPK Expression in Gastric Cancer

Cao, Shuguang; Leng, Lin; Xia, Xuanping; Wu, Hao

doi:10.1016/j.omtn.2019.04.030

Cited by 47 publications

(53 citation statements)

References 27 publications

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“…Likewise, we found that SNHG6 could bind with Ago2/miR‐101‐3p complex and negatively regulate miR‐101‐3p expression, indicating that SNHG6 might act as a sponge of miR‐101‐3p to promote NSCLC cell growth. In addition, lncRNA PTAR, SPRY4‐IT1 and LINC01303 also sponge miR‐101‐3p to prompt cell proliferation and metastasis in GC and ovarian cancer …”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p

Jiang

Xiang

et al. 2020

Thoracic Cancer

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Background: The aim of this study was to determine the function of long noncoding RNA small nucleolar RNA host gene 6 (SNHG6) in non-small cell lung cancer (NSCLC) and its underlying mechanisms. Methods: The association of SNHG6 or miR-101-3p with clinicopathological characteristics and prognosis in patents with NSCLC was assessed by TCGA dataset. Cell proliferation and invasion were evaluated by MTT and Transwell assays and SNHG6-specific binding with miR-101-3p was verified by bioinformatic analysis, luciferase gene report and RNA immunoprecipitation assays. qRT-PCR and Western blot was used to assess the effects of SNHG6 on the expression of miR-101-3p and chromodomain Y like (CDYL) in NSCLC cells. A xenograft tumor model in vivo was established to observe the effects of SNHG6 knockdown on tumor growth. Results: We found that increased expression of SNHG6 was associated with pathological stage and lymph node infiltration, and acted as an independent prognostic factor of tumor recurrence in patients with NSCLC. Silencing SNHG6 expression repressed cell growth and invasion in vitro and in vivo, but overexpression of SNHG6 reversed these effects. Furthermore, SNHG6 was identified to act as a sponge of miR-101-3p, which could reduce cell proliferation and attenuate SNHG6-induced CDYL expression. Low expression of miR-101-3p or high expression of CDYL was related to poor survival in patients with NSCLC. Conclusions: Our findings demonstrated that lncRNA SNHG6 contributed to the proliferation and invasion of NSCLC by downregulating miR-101-3p.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p

Jiang

Xiang

et al. 2020

Thoracic Cancer

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“…In GC cells over-expressing SPRY4-IT1, CSC markers, including CD73, CD146, and ALDH1, were all significantly elevated. In contrast, inhibition of SPRY4-IT1 reduced the self-renewal ability of BGC823 cells and significantly reduced the expression of CSC markers 146 . These results consistently show that SPRY4-IT1 plays a role in maintaining the GC stem cell phenotype.…”

Section: Relationships Between Lncrnas and The Hallmarks Of Gastric Cmentioning

confidence: 86%

Long non-coding RNAs in gastric cancer: New emerging biological functions and therapeutic implications

Tan

Zhang

et al. 2020

Theranostics

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Gastric cancer (GC) is currently the fourth most common malignancy and the third leading cause of cancer-related deaths worldwide. Long non-coding RNAs (lncRNAs), transcriptional products with more than 200 nucleotides, are not as well-characterized as protein-coding RNAs. Accumulating evidence has recently revealed that maladjustments of diverse lncRNAs may play key roles in multiple genetic and epigenetic phenomena in GC, affecting all aspects of cellular homeostasis, such as proliferation, migration, and stemness. However, the full extent of their functionality remains to be clarified. Considering the lack of viable biomarkers and therapeutic targets, future research should be focused on unravelling the intricate relationships between lncRNAs and GC that can be translated from bench to clinic. Here, we summarized the state-of-the-art advances in lncRNAs and their biological functions in GC, and we further discuss their potential diagnostic and therapeutic roles. We aim to shed light on the interrelationships between lncRNAs and GC with respect to their potential therapeutic applications. With better understanding of these relationships, the biological functions of lncRNAs in GC development will be exploitable, and promising new strategies developed for the prevention and treatment of GC.

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“…18 Moreover, the long non-coding RNA SPRY4 intronic transcript 1 (SPRY4-IT1), transcribed from an intronic region of SPRY4, exhibits controversial roles. SPRY4-IT1 promotes tumour progression in breast cancer, 19 gastric cancer, 20 bladder cancer, 21 and cervical cancer, 22 while suppressing cell growth and metastasis in lung cancer. 23 Recent studies have proposed that increased SPRY4-IT1 expression is related to the etiology of PE.…”

Section: Introductionmentioning

confidence: 99%

SPRY4 regulates trophoblast proliferation and apoptosis via regulating IFN‐γ‐induced STAT1 expression and activation in recurrent miscarriage

Shi

Zhang

et al. 2020

American J Rep Immunol

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Problem:The dysregulation of trophoblast functions is one of the leading causes of recurrent miscarriage (RM), which frustrates 1%-5% of couples of childbearing ages. Sprouty 4 (SPRY4) is considered as a tumour suppressor and exerts a negative role in cell viability. However, its role in regulating trophoblast behaviors at the maternalfetal interface remains largely unknown. Method of Study:First-trimester villous samples were collected from RM patients and healthy controls (HCs) to determine the SPRY4 expression in human placenta during early pregnancy. The HTR8/SVneo cell line was introduced to clarify trophoblast cell functions via transfecting with specific short interfering RNA against SPRY4 or SPRY4-overexpressing lentivirus in vitro. In addition, gene expression microarray analysis was performed to explore the downstream molecules and pathways. Results:Our results revealed that SPRY4 expression was significantly increased in the first-trimester cytotrophoblasts of RM patients compared with HCs. Furthermore, SPRY4 overexpression inhibited trophoblast proliferation and accelerated apoptosis in vitro, while SPRY4 knockdown reversed these effects. Mechanistically, IFN-γ -induced STAT1 expression and activation were involved in the regulation of trophoblast proliferation and apoptosis by SPRY4, and IFN-γ promoted SPRY4 expression and STAT1 phosphorylation through PI3K/AKT pathway. Additionally, both STAT1 and phosphorylated STAT (p-STAT) levels were also upregulated in trophoblasts from RM patients and positively correlated with SPRY4 expression. Conclusion:Our findings indicate that SPRY4 may act as a negative regulator of trophoblast functions through upregulating IFN-γ/PI3K/AKT-induced STAT1 activation. High levels of SPRY4 and STAT1 may contribute to RM development and progression, and blocking of either target could be a novel therapeutic strategy for RM patients. K E Y W O R D SIFN-γ, recurrent miscarriage, SPRY4, STAT1, trophoblast

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RETRACTED: lncRNA SPRY4-IT1 Regulates Cell Proliferation and Migration by Sponging miR-101-3p and Regulating AMPK Expression in Gastric Cancer

Cited by 47 publications

References 27 publications

Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p

Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p

Long non-coding RNAs in gastric cancer: New emerging biological functions and therapeutic implications

SPRY4 regulates trophoblast proliferation and apoptosis via regulating IFN‐γ‐induced STAT1 expression and activation in recurrent miscarriage

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