RETRACTED: LncRNA SNHG19 Promotes the Development of Non-Small Cell Lung Cancer via Mediating miR-137/E2F7 Axis

Zhao, Guochao; Ning, Zhaofeng; Wang, Rui

doi:10.3389/fonc.2021.630241

Cited by 4 publications

(2 citation statements)

References 37 publications

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“… 24 E2F7 was highly expressed in LUAD tissues and involved in the progression of NSCLC through regulating LncRNA-SNHG19. 25 E2F7 also played an important role in the Circ-AASDH/miR-140-3p/E2F7 axis and participated in regulating LUAD tumor growth. 26 A recent study reported that E2F8 expression was found to be significantly upregulated in lung cancer tissues and cells by immunofluorescence staining, and knockdown E2F8 could inhibit lung cancer cell proliferation and tumor growth.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the E2F Transcription Factor Family in Human Lung Adenocarcinoma

Wang¹,

Liu²,

Cheang³

et al. 2022

IJGM

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Background E2F transcription factors (E2Fs), code a family of pivotal transcription factors, have been identified as key regulators in tumor tumorigenesis. However, the function of E2F family in human lung adenocarcinoma (LUAD) have not been fully elucidated. Methods Herein, The Cancer Genome Atlas (TCGA) databases, Kaplan-Meier plotter, cBioPortal and TIMER were used to analyze differential expression, prognostic value, genetic alteration and immune cell infiltration of E2Fs in LUAD patients. Results The expression levels of E2Fs ( E2F1-8 ) were all significantly upregulated in LUAD tissues compared with normal lung tissues. All eight E2Fs had low rates of gene mutation in LUAD patients from cBioPortal databases. Survival analysis revealed that E2F2 (P=0.038; HR 1.36; 95% CI 1.02–1.81), E2F7 (P<0.001; HR 1.78; 95% CI 1.33–2.39) and E2F8 (P=0.03; HR 1.37; 95% CI 1.02–1.82) were significantly associated with poor prognosis. Multivariate cox regression analysis found that only E2F7 (P<0.001; HR 2.72; 95% CI 1.75–4.25) was an independent prognostic predictor in LUAD after adjusting common clinical parameters. The receiver operating characteristic (ROC) analysis also found that E2F7 had high diagnostic value for LUAD (AUC=0.901). Further analysis found that E2F7 was significantly associated with LUAD immune cell infiltration of B cell, T cell, neutrophil, and myeloid dendritic cell. E2F7 also have positive correlations with immune checkpoint genes including SIGLEC15, CD274, HAVCR2, PDCD1LG2, CTLA4, TIGIT, LAG3 and PDCD1 in LUAD. Conclusion Our findings showed various association of E2F7 in LUAD diagnostic and prognostic aspects, which suggested its potential in becoming a novel biomarker.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of the E2F Transcription Factor Family in Human Lung Adenocarcinoma

Wang¹,

Liu²,

Cheang³

et al. 2022

IJGM

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“… 9 , 10 Many special lncRNAs have been proven to be abnormally expressed and used as specific molecular markers in many human tumors. 23 , 24 SNHG16, a non-coding RNA expressed in aggressive neuroblastoma, is 2435 nucleotides long and located on chromosome 17q25.1. 13 , 25 In recent years, the role of SNHG16 in malignant tumors has been widely studied.…”

Section: Discussionmentioning

confidence: 99%

Novel lncRNA SNHG16 Promotes the Growth and Metastasis of Malignant Melanoma by Regulating miR-205-5p/PAK2 Axis

Xia¹,

Guan²,

Li³

et al. 2022

CCID

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Background Long non-coding RNAs (lncRNAs) play an key role in the biological processes of various malignant tumors. SNHG16 has been confirmed to be associated with the progression of various cancers. However, function and molecular mechanism of SNHG16 in melanoma have not been studied by scholars. Methods The expression of SNHG16 in melanoma tissues were detected by using qRT-PCR. Melanoma cases from The Cancer Genome Atlas (TCGA) and GEO#GSE15605 were included in this study. CCK-8 assay, EdU assay, transwell and scratch wound assay were used to explore the role of SNHG16 in melanoma cells. Luciferase reporter assays and RNA pull-down assay were used to explore the molecular mechanism of SNHG16 in melanoma. Results Here, we found that SNHG16 was up-regulated in melanoma. SNHG16 enhances the growth and metastasis of melanoma. SNHG16 can promote the expression of P21-activated kinases 2 (PAK2) by sponging miR-205-5p. PAK2 is the target gene of miR-205-5p. We demonstrated that SNHG16 promotes the metastasis and growth of melanoma through miR-205-5p/PAK2 axis. Conclusion This study firstly confirmed the role and mechanism of SNHG16 in the occurrence and development of melanoma. Therefore, SNHG16 may become a key point in the diagnosis, prognosis and treatment of melanoma patients in the future.

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Mechanism of lncRNA SNHG19 miR-299-5p MAPK6 signaling axis promoting metastasis of non-small cell lung cancer cells

Zhong

Qiu

2022

Oncology and Translational Medicine

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ObjectiveThe aim of this study was to explore the mechanism behind lncRNA small nucleolar RNA host gene 19 (lncRNA SNHG19)/microrNA-299-5P (miR-299-5p)/mitogen-activated protein kinase 6 (MAPK6) signaling axis promoting metastasis of non-small cell lung cancer (NSCLC).MethodsTo analyze the abnormal expression of lncRNAs in NSCLC, 50 surgically resected NSCLC and adjacent tissue samples were collected from August 2021 to August 2022. The mRNA expression levels of lncRNA SNHG19, Mir-299-5p, and MAPK6 were detected by qRT-PCR. The functions of lncRNA SNHG19, Mir-299-5p and MAPK6 were investigated by CCK-8, clone formation, EdU, scratch, Transwell western blotting (WB) and in vivo xenograft assay. RNA fluorescence in-situ hybridization (FISH), RNA pull-down, dual luciferase reporter, and RNA co-immunoprecipitation assays were used to explore the mechanism of action between lncRNA SNHG19, miR-299-5p, and MAPK6.ResultsHigh expression of lncRNA SNHG19 was correlated with poor prognosis, tumor size, lymph node metastasis, and TNM stage in NSCLC patients (P < 0.05). Cell function experiments showed that lncRNA SNHG19 could improve the proliferation, clone formation, migration, and invasion ability of A549 cells both in vitro and in vivo (all P < 0.05) and increased the relative expression levels of vimentin and MAPK6 (P <0.05). The relative expression level of E-cadherin was decreased (P < 0.05). lncRNA SNHG19 can interact with Mir-299-5p and regulate the expression level of MAPK6.ConclusionlncRNA SNHG19 is upregulated in NSCLC tissues and cells, and its high expression is associated with tumor progression and poor survival. Moreover, it can act as a molecular sponge for Mir-299-5p to regulate MAPK6 expression and promote the proliferation and metastasis of A549 cells.

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RETRACTED: LncRNA SNHG19 Promotes the Development of Non-Small Cell Lung Cancer via Mediating miR-137/E2F7 Axis

Cited by 4 publications

References 37 publications

Comprehensive Analysis of the E2F Transcription Factor Family in Human Lung Adenocarcinoma

Comprehensive Analysis of the E2F Transcription Factor Family in Human Lung Adenocarcinoma

Novel lncRNA SNHG16 Promotes the Growth and Metastasis of Malignant Melanoma by Regulating miR-205-5p/PAK2 Axis

Mechanism of lncRNA SNHG19 miR-299-5p MAPK6 signaling axis promoting metastasis of non-small cell lung cancer cells

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