RETRACTED: LncRNA SNHG16 promotes tumor growth of pancreatic cancer by targeting miR-218-5p

Liu, Songyang; Zhang, Wei; Li, Kai; Liu, Yahui

doi:10.1016/j.biopha.2019.108862

Cited by 55 publications

(45 citation statements)

References 26 publications

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“…In order to explor the effect of RNASEH1-AS1 in LUSC, we conducted the in vitro cell experiment and in vivo xenograft assay. miR-218 function as lncRNA targets and influence cancer cell malignant behaviors of many cancers, such as hepatocelluar carcinoma [20], pancreatic cancer [21] and breast cancer [22]. We found that the expression of miR-218-5p was suppressed in clinical LUSC tissues.…”

Section: Introductionmentioning

confidence: 78%

The positive feedback loop of RNASEH1-AS1/has-miR-218-5p/NET1 mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

Jia

Wang

et al. 2020

Preprint

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Background In molecular level, competing endogenous RNAs (ceRNAs) regulates other RNA transcripts through competing for shared microRNAs (miRNA). miRNA negatively regulate gene expression at the levels of mRNAs stability and translation suppression. Methods We tested the mRNA level of miR-218-5p and RNASEH1-AS1 in clinical lung squamous cell carcinoma tissues by qRT-PCR. In the exploring of the role of miR-218-5p and RNASEH1-AS1 in the malignant phenotype of NCI-H520 cells, colony formation and MTT assay were used to test the cell viability and proliferation capability, trans-well invasion and wound healing assay were performed to examine the cell migration and invasion. ChIP assay was conducted to confirm the direct interact of POU2F1 and RNASEH1-AS1 promoter. Results In this investigation, we found that LncRNA RNASEH1-AS1 is up-regulated in human lung cancer, and serves as a miRNA sponge for hsa-miR-218-5p in human lung squamous carcinoma cells. lncRNA RNASEH1-AS1 facilitates growth and motility of lung squamous carcinoma cells, while miR-218-5p does the opposite. NET1 and POU2F1 are validated as direct and functional targets of miR-218-5p. The downregulation of miR-218-5p releases the suppression of NET1 and POU2F1. POU2F1 binds directly to the lncRNA-RNASEH1-AS1 promoter and acts as transcription factor to enhance the promoter activity of RNASEH1-AS1. Conclusion Above all, the positive feedback loop of RNASEH1-AS1/ hsa-miR-218-5p/ NET1/ POU2F1 can help us to understand the regulatory mechanism behind genesis and progression of human lung squamous carcinoma, possibly providing new biomarkers for its diagnosis and treatment.

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Section: Introductionmentioning

confidence: 78%

The positive feedback loop of RNASEH1-AS1/has-miR-218-5p/NET1 mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

Jia

Wang

et al. 2020

Preprint

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“…Previous studies have demonstrated that miR-218-5p as a tumor suppressor miRNA in various types of cancers, such as gastric cancer [30], colorectal cancer [31], prostate cancer [32], and pancreatic cancer [33].…”

Section: Discussionmentioning

confidence: 99%

LncRNA HOXA-AS3 promotes the progression of oral squamous cell carcinoma through sponging miR-218-5p

Zhao

Yao

2020

Preprint

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Objective The aim of the present study was to investigate the roles and molecular mechanism of long non-coding RNA (lncRNA) HOXA-AS3 in the progression of oral squamous cell carcinoma (OSCC). Methods The expression of HOXA-AS3 and miR-218-5p was detected in OSCC tissues and cells using quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) and colony formation assays were used to examine the effects of HOXA-AS3 and miR-218-5p on the proliferation of OSCC cells. Luciferase reporter gene assay was used to confirm the directly binding condition between lncRNA HOXA-AS3 and miR-218-5p in OSCC cells. RNA immunoprecipitation assay was employed to verify the interaction between HOXA-AS3 and miR-218-5p. Results The relative expression of lncRNA HOXA-AS3 was observably upregulated in OSCC tissues and cell lines compared with the para-cancerous tissues and normal human oral keratinocyte (NHOK), respectively. Knockdown of HOXA-AS3 significantly inhibited the proliferation and colony formation of OSCC cells. Bioinformatics analysis and luciferase reporter assay showed that HOXA-AS3 directly bound to miR-218-5p. Moreover, the expression of miR-218-5p was negatively regulated by HOXA-AS3, and there was an inverse correlation between them. Silencing miR-218-5p reversed the inhibitory effect of lncRNA HOXA-AS3 knockdown on the proliferative potential of OSCC cells. Conclusion In summary, our study illustrated lncRNA HOXA-AS3 promoted cancer cell proliferation in OSCC possibly by sponging miR-218-5p for the first time, which provides a new target or a potential diagnostic biomarker of the treatment for OSCC.

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“…Moreover, increased expression of SNHG16 was correlated with poor differentiation, advanced TNM stage, lymph node metastasis, and shorter survival. 41 SNHG16 is upregulated in both papillary thyroid tissues and cell lines, which indicates advanced TNM stage and lymph node metastasis. 42 In addition to sequencing in tumor tissues, lncRNA levels can also be measured in exosomes.…”

Section: Overexpression Of Snhg16 and Clinical Significance In Cancermentioning

confidence: 99%

<p><em>SNHG16</em>: A Novel Long-Non Coding RNA in Human Cancers</p>

Yang

Wei

2019

OTT

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Long noncoding RNAs (lncRNAs) have recently been considered as central regulators in diverse biological processes controlling tumorigenesis. Small nucleolar RNA host gene 16 (SNHG16) is an important tumor-associated lncRNA mainly involved in tumorigenesis and progression by competing with endogenous RNA (ceRNA) which sponges tumor-suppressive microRNA (miRNA), and by its recruitment mechanism. SNHG16 is overexpressed in tumor tissues and cell lines of different kinds of cancers, and its presence is associated with a poor clinical prognosis. Reviewing all publications about SNHG16 revealed that it plays a key role in the different hallmarks that define human cancer, including promoting proliferation, activating migration and invasion, inhibiting apoptosis, affecting lipid metabolism and chemoresistance. This review highlights the role that the aberrant expression of SNHG16 plays in the development and progression of cancer, and suggests that SNHG16 may function as a potential biomarker and therapeutic target for human cancers.

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RETRACTED: LncRNA SNHG16 promotes tumor growth of pancreatic cancer by targeting miR-218-5p

Cited by 55 publications

References 26 publications

The positive feedback loop of RNASEH1-AS1/has-miR-218-5p/NET1 mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

The positive feedback loop of RNASEH1-AS1/has-miR-218-5p/NET1 mediated by POU2F1 contributes to the development and progression of human lung squamous carcinoma

LncRNA HOXA-AS3 promotes the progression of oral squamous cell carcinoma through sponging miR-218-5p

<p><em>SNHG16</em>: A Novel Long-Non Coding RNA in Human Cancers</p>

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