RETRACTED: Knockdown of lncRNA CCAT2 inhibits endometrial cancer cells growth and metastasis via sponging miR-216b

Xie, Pengmu; Cao, Hongying; Liu, Ying; Wang, Jianhua; Cui, Zhonghui

doi:10.3233/cbm-170388

Cited by 48 publications

(40 citation statements)

References 32 publications

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“…20 Previous studies have proved that CCAT2 knockdown significantly inhibits the migration and invasion of MKN45 cells (GC), 10 LCC9/MCF-7 cells (breast cancer), 21 and HEC-1-A/RL95-2 cells (endometrial cancer). 22 In this study, we found that siRNA-CCAT2 transfection significantly decreased the numbers of migrant and invasive cells. Our findings are just consistent with previous studies, and further illustrate that silencing of CCAT2 inhibits the migration and invasion of GC cells.…”

Section: Discussionmentioning

confidence: 53%

<p>Silencing of Long Non-Coding RNA Colon Cancer-Associated Transcript 2 Inhibits the Growth and Metastasis of Gastric Cancer Through Blocking mTOR Signaling</p>

Shi¹,

Wang²,

Yang³

et al. 2020

OTT

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Purpose: This study aimed to evaluate the specific role of colon cancer-associated transcript 2 (CCAT2) on gastric cancer (GC), and reveal the potential regulatory mechanism relating to mammalian target of rapamycin (mTOR) signaling. Methods: The expression of CCAT2 was detected in GC tissues and cells by quantitative real-time PCR (qRT-PCR), and its relation with the pathologic characteristics of GC patients was analyzed. HGC-27 and SGC-7901 cells were transfected with siRNA-CCAT2 to silence CCAT2, and HGC-27 cells were then treated with an mTOR agonist Leucine (Leu) to activate mTOR signaling. The cell proliferation was evaluated by cell viability and colony formation. The cell cycle and apoptosis, and the migration and invasion abilities were detected by Flow cytometry, and Transwell assay, respectively. The expression of PCNA (proliferation marker), Snail, N-cadherin, E-cadherin (invasion markers), P53, Caspase-8, Bcl-2 (apoptosis markers), LC3-II/LC3-I, ATG3, p62 (autophagy makers), phosphorylated mTOR (p-mTOR), p-AKT, and p-p70S6K (mTOR signaling markers) were detected by Western blot. Results: CCAT2 was upregulated in GC tissues and cells, and positively associated with the maximum tumor diameter, lymphatic metastasis, TNM staging, and low overall survival rate (P < 0.05). siRNA-CCAT2 transfection significantly inhibited the viability, colony formation, and migration and invasion abilities, blocked the cell cycle in G0/G1 phase, and promoted the apoptosis and autophagy of SGC-7901 and HGC-27 cells (P < 0.05). In addition, siRNA-CCAT2 transfection significantly upregulated P53, Caspase-8, LC3-II/LC3-I and ATG3, and downregulated PCNA, Bcl-2, p62, p-mTOR, p-AKT and p-p70S6K in SGC-7901 and HGC-27 cells (P < 0.05). siRNA-CCAT2 reversed the tumor-promoting effect of mTOR signaling activation on HGC-27 cells (P < 0.05). Conclusion: Silencing of CCAT2 inhibited the proliferation, migration and invasion, and promoted the apoptosis and autophagy of GC cells through blocking mTOR signaling.

show abstract

Section: Discussionmentioning

confidence: 53%

<p>Silencing of Long Non-Coding RNA Colon Cancer-Associated Transcript 2 Inhibits the Growth and Metastasis of Gastric Cancer Through Blocking mTOR Signaling</p>

Shi¹,

Wang²,

Yang³

et al. 2020

OTT

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show abstract

“…[2017] also provided evidence that miR‐216b inhibited glioma cell migration and invasion through suppression of FOXM1. In endometrial cancer, knockdown of lncRNA CCAT2 inhibited cell growth and metastasis via sponging miR‐216b [Xie et al., ]. The study performed by Yao et al.…”

Section: Resultsmentioning

confidence: 99%

“…Zhang et al [2017] also provided evidence that miR-216b inhibited glioma cell migration and invasion through suppression of FOXM1. In endometrial cancer, knockdown of lncRNA CCAT2 inhibited cell growth and metastasis via sponging miR-216b [Xie et al, 2017]. The study performed by Yao et al [2018] showed that miR-216b suppressed colorectal cancer proliferation, migration and invasion by targeting SRPK1.…”

Section: Research Articlementioning

confidence: 99%

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

Sun

Geng

et al. 2018

Biology of the Cell

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Background Information To characterise Linc00518 expression in prostate cancer and elucidate the potential mechanistic involvement in paclitaxel resistance, the relative expression of Linc00518 and miR‐216b‐5p was determined by real‐time PCR. The regulatory effect of miR‐216b‐5p on either Linc00518 or GATA6 was interrogated with luciferase reporter assay. The endogenous GATA6 protein was analysed by Western blotting. The cell viability was measured by MTT assay and IC50 of paclitaxel was calculated through cell counting. Results Linc00518 was highly expressed in prostate tumour both in vivo and in vitro. High level of Linc00518 transcripts associated with paclitaxel resistance. Linc00518 competitively inhibited miR‐216b‐5p through sponging mechanism. Linc00518 deficiency compromised the paclitaxel resistance in the acquired resistance cell lines. Conclusions and Significance We demonstrated that overexpression of Linc00518 contributed to the paclitaxel resistance in prostate cancer via sequestering miR‐216b‐5p.

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“…CCAT2, as a novel oncogene, is located in the hotspot of tumour-related rs6983267 SNP, which promotes the occurrence and development of kinds of cancers by affecting Wnt and TGF-b signalling pathways [9,19]. Such as Xie et al reported that CCAT2 was overexpressed in endometrial cancer and knockdown of CCAT2 inhibited endometrial cancer cells growth and metastasis via sponging miR-216b [20]. Ruan et al also demonstrated that CCAT2 was able to promote the progression of OSA by enhancing cell proliferation via GSK3b/b-catenin signalling pathway [21].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA CCAT2 acts as an oncogene in osteosarcoma through regulation of miR-200b/VEGF

Liu

Kong

Sun

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background/aim: Colon cancer-associated transcript 2 (CCAT2) is a new lncRNA, which is closely associated with risk of several cancers. The aim of this study was to explore the regulatory mechanism of CCAT2 in osteosarcoma (OSA). Methods: Cells were transfected with si-CCAT2, microRNA (miR)-200b inhibitor and the corresponding controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the expression of CCAT2 and miR-200b in OSA tissues and cell lines. CCK8 and bromodeoxyuridine (BrdU) were conducted to examine cell proliferation. Apoptosis was detected by PI/FITC-Annexin V combining with flow cytometric analysis. Migration and invasion were respectively measured through transwell chambers assays. Western blot was used to examine expressions of relative proteins. Results: CCAT2 was highly expressed and miR-200b was lowly expressed in OSA tissues and cell lines. Knockdown of CCAT2 suppressed cell proliferation, migration and invasion but induced apoptosis and up-regulation of miR-200b. miR-200b inhibitor weakened the effect of si-CCAT2 on cell progression and cell mobility. Besides, knockdown of CCAT2 blocked the PI3K/Akt and AMPK pathways through upregulating miR-200b. Conclusions: The CCAT2/miR-200b/vascular endothelial growth factor (VEGF) axis plays important regulating effect in OSA through the PI3K/Akt and AMPK pathways.

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RETRACTED: Knockdown of lncRNA CCAT2 inhibits endometrial cancer cells growth and metastasis via sponging miR-216b

Abstract: To conclude, these results demonstrated lncRNA CCAT2 was highly expressed in endometrial cancer tissues. Knockdown of CCAT2 inhibited cell growth and metastasis of endometrial cancer cells by sponging miR-216b.

Cited by 48 publications

References 32 publications

<p>Silencing of Long Non-Coding RNA Colon Cancer-Associated Transcript 2 Inhibits the Growth and Metastasis of Gastric Cancer Through Blocking mTOR Signaling</p>

<p>Silencing of Long Non-Coding RNA Colon Cancer-Associated Transcript 2 Inhibits the Growth and Metastasis of Gastric Cancer Through Blocking mTOR Signaling</p>

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

RETRACTED ARTICLE: Long non-coding RNA CCAT2 acts as an oncogene in osteosarcoma through regulation of miR-200b/VEGF

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