RETRACTED: Gene expression markers for Caenorhabditiselegans vulval cells

Inoué, Takao; Sherwood, David R.; Aspöck, Gudrun; Butler, James A.; Gupta, Bhagwati P.; Kirouac, Martha; Wang, Minqin; Lee, Pei-Yun; Kramer, James M.; Hope, Ian A.; Bürglin, Thomas R.; Sternberg, Paul W.

doi:10.1016/s1567-133x(02)00055-8

“…Following eversion, the vulva lumen remains in a closed conformation unless opened by contractions of the sex muscles, which attach to multiple vulva toroids ( Sharma-Kishore et al, 1999 ). During L4 and adult stages, each vulva cell type expresses different combinations of known transcription factors, membrane fusogens, or other molecular markers ( Inoue et al, 2004 ; Inoue et al, 2002 ; Inoue et al, 2005 ; Mok et al, 2015 ; Shemer et al, 2000 ; Sternberg and Horvitz, 1989 ), but the biological distinctions among the seven cell types are not well understood.…”

Section: Resultsmentioning

confidence: 99%

A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in Caenorhabditis elegans

Cohen

¹

,

Sparacio

²

,

Belfi

³

et al. 2020

eLife

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Biological tubes must develop and maintain their proper diameter in order to transport materials efficiently. These tubes are molded and protected in part by apical extracellular matrices (aECMs) that line their lumens. Despite their importance, aECMs are difficult to image in vivo and therefore poorly understood. The C. elegans vulva has been a paradigm for understanding many aspects of organogenesis. Here we describe the vulva luminal matrix, which contains chondroitin proteoglycans, Zona Pellucida (ZP) domain proteins, and other glycoproteins and lipid transporters related to those in mammals. Confocal and transmission electron microscopy revealed, with unprecedented detail, a complex and dynamic aECM. Different matrix factors assemble on the apical surfaces of each vulva cell type, with clear distinctions seen between Ras-dependent (1˚) and Notch-dependent (2˚) cell types. Genetic perturbations suggest that chondroitin and other aECM factors together generate a structured scaffold that both expands and constricts lumen shape.

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“…6G). Next, eggs from the transgenic strain SU93, which express the apical junction‐associated AJM‐1‐GFP chimeric protein and can evaluate vulva cell fusion during L4 stage (Gupta et al 2003; Hurd and Kemphues 2003; Inoue et al 2002), were treated with and without 76 . As compared to controls, treatment with 76 caused an apparent defect in vulva cell fusion as shown by the 2 fold inhibition of the joining of the two symmetric halves of homologous cells that will form the functional vulva structure (Fig.…”

Section: Resultsmentioning

confidence: 99%

Using Caenorhabditis elegans as a model organism for evaluating extracellular signal-regulated kinase docking domain inhibitors

Chen

¹

,

MacKerell

²

,

Luo

³

et al. 2008

J. Cell Commun. Signal.

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We have recently identified several novel ATPindependent inhibitors that target the extracellular signalregulated kinase-2 (ERK2) protein and inhibit substrate phosphorylation. To further characterize these compounds, we describe the use of C. elegans as a model organism. C. elegans is recognized as a versatile and cost effective model for use in drug development. These studies take advantage of the well characterized process of vulva development and egg laying, which requires MPK-1, the homolog to human ERK2. It is shown that treatment of C. elegans eggs or larvae prior to vulva formation with a previously identified lead compound (76) caused up to 50% reduction in the number of eggs produced from the adult worm. In contrast, compound 76 had no effect on egg laying in young adult or adult worms with fully formed vulva. The reduction in egg laying by the test compound was not due to effects on C. elegans life span, general toxicity, or non-specific stress. However, compound 76 did show selective inhibition of phosphorylation of LIN-1, a MPK-1 substrate essential for vulva precursor cell formation. Moreover, compound 76 inhibited cell fusion necessary for vulva maturation and reduced the multivulva phenotype in LET-60 (Ras) mutant worms that have constitutive activation of MPK-1. These findings support the use of C. elegans as a model organism to evaluate the selectivity and specificity of novel ERK targeted compounds.

show abstract

“…Following eversion, the vulva lumen remains in a closed conformation unless opened by contractions of the sex muscles, which attach to multiple vulva toroids (Sharma- Kishore et al, 1999). During L4 and adult stages, each vulva cell type expresses different combinations of known transcription factors, membrane fusogens, or other molecular markers (Inoue et al, 2004;Inoue et al, 2002;Inoue, Wang, Ririe, Fernandes, & Sternberg, 2005;Mok et al, 2015;Shemer et al, 2000;Sternberg & Horvitz, 1989), but the biological distinctions among the seven cell types are not well understood.…”

Section: Background: Vulva Tube Formationmentioning

confidence: 99%

A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen inCaenorhabditis elegans

Cohen

¹

,

Sparacio

²

,

Belfi

³

et al. 2020

Preprint

View full text Add to dashboard Cite

Biological tubes must develop and maintain their proper diameter in order to transport materials efficiently. These tubes are molded and protected in part by apical extracellular matrices (aECMs) that line their lumens. Despite their importance, aECMs are difficult to image in vivo and therefore poorly understood. The C. elegans vulva has been a paradigm for understanding many aspects of organogenesis. Here we describe the vulva luminal matrix, which contains chondroitin proteoglycans, Zona Pellucida (ZP) domain proteins, and other glycoproteins and lipid transporters related to those in mammals. Confocal and transmission electron microscopy revealed, with unprecedented detail, a complex and dynamic aECM. Different matrix factors assemble on the apical surfaces of each vulva cell type, with clear distinctions seen between Ras-dependent (1˚) and Notch-dependent (2˚) cell types. Genetic perturbations suggest that chondroitin and other aECM factors together generate a structured scaffold that both expands and constricts lumen shape.

show abstract

RETRACTED: Gene expression markers for Caenorhabditiselegans vulval cells

Cited by 26 publications

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A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in Caenorhabditis elegans

A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in Caenorhabditis elegans

Using Caenorhabditis elegans as a model organism for evaluating extracellular signal-regulated kinase docking domain inhibitors

A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen inCaenorhabditis elegans

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