RETRACTED: Expression system for heterologous protein expression in the filamentous fungus Aspergillus unguis

Madhavan, Aravind; Pandey, Ashok; Sukumaran, Rajeev

doi:10.1016/j.biortech.2017.05.140

Cited by 27 publications

(13 citation statements)

References 28 publications

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“…are commonly secreted with native proteins, such as glucoamylases in Aspergillus sp. [11]. Genetic modifications by knocking out proteases and the majority of extracellular proteins, such as cellulase Cel7A/Cel6A, or multiple-round mutagenesis, were performed in the host strain and attempted to gain a cleaner background for target proteins expression [24].…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the key factor for the high yield of proteins or natural products is mostly the selected strong promoters. Constitutive promoters, such as gpdA(p) or tef1(p), have been reported and widely used for protein expression in filamentous fungi [9,11]. However, further improvements for strong constitutive promoters have been restricted since their regulatory network in the host remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium

Zhang

Xiang

et al. 2020

Microb Cell Fact

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Background: Filamentous fungi have long been used as hosts for the production of proteins, enzymes and valuable products in various biotechnological applications. However, recombinant proteins are expressed with highly secreted host proteins when stronger promoters are used under inducing conditions. In addition, the efficiency of target protein expression can be limited by the application of constitutive promoters in recently developed filamentous fungal expression systems. Results: In this study, a novel expression system was constructed by using a Penicillium oxalium strain that has powerful protein secretion capability. The secretory background of the host was reduced by knocking out the Amy13A protein and utilizing the starch as a carbon source. The strong promoter amy15A(p) was further improved by overexpressing the transcription activator AmyR and deleting of putative repressor CreA. By using the native amylase Amy15A as a reporter, the efficiency of expression from the amy15A promoter was dramatically and specifically enhanced after redesigning the regulatory network of amylase expression. Conclusions: Our researches clearly indicated that the triple-gene recombinant strain Δ13A-OamyR-ΔCreA, with the amy15A(p) promoter could be used as a suitable expression system especially for high-level and high-purity protein production.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium

Zhang

Xiang

et al. 2020

Microb Cell Fact

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“…In another study, cellobiohyrolase promoter from Trichoderma reesei has been used as hybrid promoter along with LAC4 promoter of K. lactis for the production of GFP [ 39 ]. Engineered LAC4 promoter with fungal signal peptide has been used for the production of human interferon β [ 40 – 42 ].…”

Section: Promoter Engineeringmentioning

confidence: 99%

Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

et al. 2021

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The manufacture of recombinant therapeutics is a fastest-developing section of therapeutic pharmaceuticals and presently plays a significant role in disease management. Yeasts are established eukaryotic host for heterologous protein production and offer distinctive benefits in synthesising pharmaceutical recombinants. Yeasts are proficient of vigorous growth on inexpensive media, easy for gene manipulations, and are capable of adding post translational changes of eukaryotes. Saccharomyces cerevisiae is model yeast that has been applied as a main host for the manufacture of pharmaceuticals and is the major tool box for genetic studies; nevertheless, numerous other yeasts comprising Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Yarrowia lipolytica have attained huge attention as non-conventional partners intended for the industrial manufacture of heterologous proteins. Here we review the advances in yeast gene manipulation tools and techniques for heterologous pharmaceutical protein synthesis. Application of secretory pathway engineering, glycosylation engineering strategies and fermentation scale-up strategies in customizing yeast cells for the synthesis of therapeutic proteins has been meticulously described.

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“…Besides, the secretion of heterologous protein could also be increased by overexpression of foldases, signal peptides, and chaperones via facilitated protein folding ( Goedegebuur et al, 2014 ; Nevalainen and Peterson, 2014 ). The application of heterologous signal peptides led to a high level of protein secretion; when enhanced green fluorescent protein served as a model, the heterologous protein human interferon β was finally successfully expressed in Aspergillus unguis ( Madhavan et al, 2017 ). The porin B signal peptide in Corynebacterium glutamicum was used to improve the production of model protein endoxylanase with activity in high efficiency with a performance of 615 mg/L ( An et al, 2013 ).…”

Section: Protein Level Regulationmentioning

confidence: 99%

Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories

Zhang

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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Filamentous fungi are important eukaryotic organisms crucial in substrate degradation and carbon cycle on the earth and have been harnessed as cell factories for the production of proteins and other high value-added products in recent decades. As cell factories, filamentous fungi play a crucial role in industrial protein production as both native hosts and heterologous hosts. In this review, the regulation strategies of carbohydrate active enzyme expression at both transcription level and protein level are introduced, and the transcription regulations are highlighted with induction mechanism, signaling pathway, and promoter and transcription factor regulation. Afterward, the regulation strategies in protein level including suitable posttranslational modification, protein secretion enhancement, and protease reduction are also presented. Finally, the challenges and perspectives in this field are discussed. In this way, a comprehensive knowledge regarding carbohydrate active enzyme production regulation at both transcriptional and protein levels is provided with the particular goal of aiding in the practical application of filamentous fungi for industrial protein production.

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RETRACTED: Expression system for heterologous protein expression in the filamentous fungus Aspergillus unguis

Cited by 27 publications

References 28 publications

Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium

Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium

Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories

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