RETRACTED: EPO protects mesenchymal stem cells from hyperglycaemic injury via activation of the Akt/FoxO3a pathway

Cui, Jinjin; Liu, Xiaohong; Zhang, Zhuoqi; Xuan, Yongli; Liu, Xinxin; Zhang, Fengyun

doi:10.1016/j.lfs.2018.12.045

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…The application of EPO on ROS-induced RPE cytotoxicity in vitro have been shown to provide protective effects (Wang et al, 2009). Not only that, the EPO was also shown to act on the MSC itself by preserving stemness (Güven Bagla et al, 2018) and even protecting the cells from hyperglycemic injury (Cui et al, 2019). Furthermore, the application of rat MSCs EPO against retinal degeneration were shown to be effective in vivo (Guan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Mitigation of Sodium Iodate-Induced Cytotoxicity in Retinal Pigment Epithelial Cells in vitro by Transgenic Erythropoietin-Expressing Mesenchymal Stem Cells

Koh

Subbiah

Farhana

et al. 2021

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSC) have shown promise in restoring the vision of patients in clinical trials. However, this therapeutic effect is not observed in every treated patient and is possibly due to the inefficacies of cell delivery and high cell death following transplantation. Utilizing erythropoietin can significantly enhance the regenerative properties of MSCs and hence improve retinal neuron survivability in oxidative stress. Hence, this study aimed to investigate the efficacy of conditioned medium (CM) obtained from transgenic human erythropoietin-expressing MSCs (MSCEPO) in protecting human retinal pigment epithelial cells from sodium iodate (NaIO3)-induced cell death. Human MSC and MSCEPO were first cultured to obtain conditioned media (CM). The IC50 of NaIO3 in the ARPE-19 culture was then determined by an MTT assay. After that, the efficacy of both MSC-CM and MSC-CMEPO in ARPE-19 cell survival were compared at 24 and 48 h after NaIO3 treatment with MTT. The treatment effects on mitochondrial membrane potential was then measured by a JC-1 flow cytometric assay. The MTT results indicated a corresponding increase in cell survivability (5–58%) in the ARPE-19 cell cultures. In comparison to MSC-CM, the use of conditioned medium collected from the MSC-CMEPO further enhanced the rate of ARPE-19 survivability at 24 h (P < 0.05) and 48 h (P < 0.05) in the presence of NaIO3. Furthermore, more than 90% were found viable with the JC-1 assay after MSC-CMEPO treatment, showing a positive implication on the mitochondrial dynamics of ARPE-19. The MSC-CMEPO provided an enhanced mitigating effect against NaIO3-induced ARPE-19 cell death over that of MSC-CM alone during the early phase of the treatment, and it may act as a future therapy in treating retinal degenerative diseases.

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Section: Discussionmentioning

confidence: 99%

Mitigation of Sodium Iodate-Induced Cytotoxicity in Retinal Pigment Epithelial Cells in vitro by Transgenic Erythropoietin-Expressing Mesenchymal Stem Cells

Koh

Subbiah

Farhana

et al. 2021

Front. Cell Dev. Biol.

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“…In literature, the involvement of Akt in MSCs' senescence machinery has been reported [203] in which the accumulation of ROS may contributes to the phosphorylation of Akt on IL-8 knockdown-dependent senescence in MSCs derived from the placenta [204]. On the other hand, Akt could be used by erythropoietin to protect MSCs from hyperglycemia-induced senescence through FOXOa [205]. Also, Akt activation in vitro and in vivo was involved in MSCs' rejuvenation activity of neuron-derived neurotropic factor (NDNF), which proved to have an anti-aging effect on aged MSCs to promote the function of the injured heart [206].…”

Section: Miscellaneous Signalingmentioning

confidence: 99%

Aging of mesenchymal stem cell: machinery, markers, and strategies of fighting

et al. 2022

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Human mesenchymal stem cells (MSCs) are primary multipotent cells capable of differentiating into osteocytes, chondrocytes, and adipocytes when stimulated under appropriate conditions. The role of MSCs in tissue homeostasis, aging-related diseases, and cellular therapy is clinically suggested. As aging is a universal problem that has large socioeconomic effects, an improved understanding of the concepts of aging can direct public policies that reduce its adverse impacts on the healthcare system and humanity. Several studies of aging have been carried out over several years to understand the phenomenon and different factors affecting human aging. A reduced ability of adult stem cell populations to reproduce and regenerate is one of the main contributors to the human aging process. In this context, MSCs senescence is a major challenge in front of cellular therapy advancement. Many factors, ranging from genetic and metabolic pathways to extrinsic factors through various cellular signaling pathways, are involved in regulating the mechanism of MSC senescence. To better understand and reverse cellular senescence, this review highlights the underlying mechanisms and signs of MSC cellular senescence, and discusses the strategies to combat aging and cellular senescence. Graphical Abstract

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DNA damage response manages cell cycle restriction of senile multipotent mesenchymal stromal cells

Lin

et al. 2019

Mol Biol Rep

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RETRACTED: EPO protects mesenchymal stem cells from hyperglycaemic injury via activation of the Akt/FoxO3a pathway

Cited by 6 publications

References 31 publications

Mitigation of Sodium Iodate-Induced Cytotoxicity in Retinal Pigment Epithelial Cells in vitro by Transgenic Erythropoietin-Expressing Mesenchymal Stem Cells

Mitigation of Sodium Iodate-Induced Cytotoxicity in Retinal Pigment Epithelial Cells in vitro by Transgenic Erythropoietin-Expressing Mesenchymal Stem Cells

Aging of mesenchymal stem cell: machinery, markers, and strategies of fighting

DNA damage response manages cell cycle restriction of senile multipotent mesenchymal stromal cells

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