RETRACTED: Effects of salvianolic acid B on survival, self-renewal and neuronal differentiation of bone marrow derived neural stem cells

Zhang, Nan; Kang, Ting-Guo; Yang, Xia; Wen, Qin; Zhang, Xiaodan; Li, Hongyan; Hu, Yv; Hao, Haiguang; Zhao, Dan; Sun, Dong Il; Yan, Yaping; Zhang, Guang‐Xian; Yang, Jingxian

doi:10.1016/j.ejphar.2012.10.008

Cited by 47 publications

(34 citation statements)

References 34 publications

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“…Subsequently, cells were divided into five groups: GFP (transduced with GFP), APP (transduced with APP), APP+Ost, APP+miR-9 inhibitor, APP+miR-9 inhibitor+Ost. After 72 h of transfection, NSCs were cultured in differentiation medium composed of DMEM/F12 supplemented with 10% FBS plus 1% P/S for 10–14 days (Zhang et al, 2012). The medium was changed every 3 days.…”

Section: Methodsmentioning

confidence: 99%

Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer's Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice

Gao

Wang

et al. 2017

Front. Neurosci.

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Alzheimer's disease (AD) is the most serious neurodegenerative disease worldwide and is characterized by progressive cognitive impairment and multiple neurological changes, including neuronal loss in the brain. However, there are no available drugs to delay or cure this disease. Consequently, neuronal replacement therapy may be a strategy to treat AD. Osthole (Ost), a natural coumarin derivative, crosses the blood-brain barrier and exerts strong neuroprotective effects against AD in vitro and in vivo. Recently, microRNAs (miRNAs) have demonstrated a crucial role in pathological processes of AD, implying that targeting miRNAs could be a therapeutic approach to AD. In the present study, we investigated whether Ost could enhance cell viability and prevent cell death in amyloid precursor protein (APP)-expressing neural stem cells (NSCs) as well as promote APP-expressing NSCs differentiation into more neurons by upregulating microRNA (miR)-9 and inhibiting the Notch signaling pathway in vitro. In addition, Ost treatment in APP/PS1 double transgenic (Tg) mice markedly restored cognitive functions, reduced Aβ plague production and rescued functional impairment of hippocampal neurons. The results of the present study provides evidence of the neurogenesis effects and neurobiological mechanisms of Ost against AD, suggesting that Ost is a promising drug for treatment of AD or other neurodegenerative diseases.

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Section: Methodsmentioning

confidence: 99%

Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer's Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice

Gao

Wang

et al. 2017

Front. Neurosci.

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“…57) Numerous small molecular materials such as growth factors, retinoic acid and TCM active constituent have been well known to possess the ability to regulate the biological characteristics of NSCs and promote neurogenesis. 31) Salvianolic acid B, for example, could promote NSCs proliferation in a dose dependent manner 8) ; Ginsenoside Rb1 and Rg1 improved spatial learning and increase hippocampal synaptophysin level in mice. 58) Curcumin stimulated adult hippocampal neurogenesis and enhanced neural plasticity and repair.…”

Section: Discussionmentioning

confidence: 99%

“…After 3 d of co-culture, cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay as previously described. 8) Briefly, NSCs were incubated with 0.5 mg/ mL MTT (Invitrogen) at 37°C for 4 h following treatment with Ost, then media was removed and 100 µL of dimethyl sulfoxide (DMSO) was added to dissolve the formed formazan product. The amount of MTT formazan product was determined by measuring optical density (OD) value with a microplate reader (Thermo Fisher Scientific Inc., U.S.A.) at 580 nm.…”

Section: Preparation Of Ostmentioning

confidence: 99%

“…[5][6][7] However, poor survival and limited neuronal differentiation of the transplanted NSCs in central nervous system (CNS) remain critical limitations for developing therapeutic strategies. 7,8) The adult CNS, especially the injured CNS, may provide a relatively non-permissive environment for engrafted NSCs. 7,[9][10][11] Even under the best circumstances, the cell survival in injured CNS has been estimated to be near 10%, 12,13) and few cells differentiate into mature neuronal phenotypes.…”

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confidence: 99%

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The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

Kong

Yao

et al. 2015

Biological & Pharmaceutical Bulletin

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It is believed that neuronal death caused by abnormal deposition of amyloid-beta peptide is the major cause of the cognitive decline in Alzheimer's disease. Adult neurogenesis plays a key role in the rescue of impaired neurons and amelioration of cognitive impairment. In the present study, we demonstrated that osthole, a natural coumarin derivative, was capable of promoting neuronal stem cell (NSC) survival and inducing NSC proliferation in vitro. In osthole-treated APP/PS1 transgenic mice, a significant improvement in learning and memory function was seen, which was associated with a significant increase in the number of new neurons (Ki67 /NF-M ) and a decrease in apoptotic cells in the hippocampal region of the brain. These observations suggested that osthole promoted NSC proliferation, supported neurogenesis, and thus efficiently rescued impaired neurons in the hippocampus and ameliorated cognitive impairment. We also found that osthole treatment activated the Notch pathway and upregulated the expression of self-renewal genes Notch 1 and Hes 1 mRNA in NSCs. However, when Notch activity was blocked by the γ-secretase inhibitor DAPT, the augmentation of Notch 1 and Hes 1 protein was ameliorated, and the proliferation-inducing effect of osthole was abolished, suggesting that the effects of osthole are at least in part mediated by activation of the Notch pathway.

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“…Traditionally, Sal B was used to treat cerebrovascular, cardiovascular and neurodegenerative disorders [85,86,87]. Mice receiving Sal B (25 mg/kg) intracerebroventricularly showed significantly improved spatial learning and also memory improvement and also increased the expression levels of anti-inflammatory cytokines IL-10 and TGFβ, suppressed the pro-inflammatory cytokines IL-1β and TNFα [88] and also decreased the inflammatory astrocytes and microglial cells [54,89]. Furthermore, Sal B promotes cell growth and differentiation by activating ERK and PI3K/AKT pathways [90].…”

Section: Neurogenic Potential Of Traditional Chinese Medicine (Tcmmentioning

confidence: 99%

Neurogenic Traditional Chinese Medicine as a Promising Strategy for the Treatment of Alzheimer’s Disease

Sreenivasmurthy

Liu

Song

et al. 2017

IJMS

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Hippocampal neurogenesis plays a critical role in the formation of new neurons during learning and memory development. Attenuation of neurogenesis in the brain is one of the primary causes of dementia in Alzheimer’s disease (AD), and, conversely, modulating the process of hippocampal neurogenesis benefit patients with AD. Traditional Chinese medicine (TCM), particularly herbal medicine, has been in use for thousands of years in Asia and many regions of the world for the treatment of cancer, cardiovascular diseases and neurodegenerative diseases. In this review, we summarize the role of neurotrophic factors, signal transducing factors, epigenetic modulators and neurotransmitters in neurogenesis, and we also discuss the functions of several Chinese herbs and their active molecules in activating multiple pathways involved in neurogenesis. TCM herbs target pathways such as Notch, Wnt, Sonic Hedgehog and receptor tyrosine kinase pathway, leading to activation of a signaling cascade that ultimately enhances the transcription of several important genes necessary for neurogenesis. Given these pathway activating effects, the use of TCM herbs could be an effective therapeutic strategy for the treatment of AD.

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RETRACTED: Effects of salvianolic acid B on survival, self-renewal and neuronal differentiation of bone marrow derived neural stem cells

Cited by 47 publications

References 34 publications

Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer's Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice

Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer's Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

Neurogenic Traditional Chinese Medicine as a Promising Strategy for the Treatment of Alzheimer’s Disease

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