RETRACTED: Effects of Nigella sativa on immune responses and pathogenesis of avian influenza (H9N2) virus in turkeys

Umar, Sajid; Rehman, Abdul; Younus, Muhammad; Qamar-un-Nisa,; Ali, Asif; Shahzad, Muhammad Khurram; Shah, Muhammad Ali; Munir, Muhammad; Aslam, Hassan Bin; Yaqoob, Muhammad

doi:10.3382/japr/pfv070

Cited by 17 publications

(8 citation statements)

References 25 publications

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“…With recent cloning of avian cytokine genes, their utilisation as therapeutic agents for viral diseases in poultry as well as for vaccine adjuvants has become more feasible in the future (Umar et al, 2015b). Furthermore, immunomodulatory products have been shown to reduce the severity of some virus-induced lesions and consequently the immunosuppression (Umar et al, 2015d). Currently, MD, IBD and CIA are three major immunosuppressive diseases in poultry.…”

Section: Immunosuppression Interventionsmentioning

confidence: 99%

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

Umar

Munir

Ahsan

et al. 2017

World's Poultry Science Journal

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Poultry can be exposed to different kinds of immunosuppressive agents that impair health and welfare by destroying innate and acquired immunity leading to diminished genetic potential of poultry for efficient production. Immunosuppression is a condition characterised by humoral and cellular immune dysfunction that leads to increased susceptibility to secondary infections and vaccine failure. Immune dysfunction at the humoral level is largely due to change in soluble factors mediated by complement or chemokines for innate immunity or due to alterations in antibodies or cytokines for adaptive immunity. In contrast, immune dysfunctions at cellular levels include alterations in neutrophils, monocyte/macrophage, and natural killer cells for innate immunity or changes in B or T lymphocytes for adaptive immunity. In poultry, stressinduced immunosuppression is manifested by failure in vaccination, and increased morbidity and mortality of flocks. Immunosuppressive agents can have cytolytic effects on lymphocyte populations leading to atrophied and depleted lymphoid organs. Immunosuppression can be due to infectious agents or noninfectious agents or due to a combination of them. At present, several modern cellular and molecular approaches are being used to determine the status of the immune system during stress and disease. Comprehensive methodologies for the evaluation of immunosuppression by combined non-infectious and infectious aetiologies have not found general application. Currently, investigations are being developed in order to detect genetic expression of immunologic mediators and receptors by microarray technology. It is likely that this new technique will initiate the development of new strategies for the control and prevention of immunosuppression in poultry. A long term immunosuppression preventive approach involves genetic selection for resistance to immunosuppressive diseases.

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Section: Immunosuppression Interventionsmentioning

confidence: 99%

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

Umar

Munir

Ahsan

et al. 2017

World's Poultry Science Journal

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“…Indeed, when combined with live vaccine, inactivated NDV emulsified in BSO induced the mean HI titre of log 2 8.73 ± 0.50 which is comparable to the titre in birds vaccinated with commercial NDV vaccine. Thus, given its low toxicity and profound immunomodulatory effects, as established by previous researchers (Salem 2005;Umar et al 2015;Zaoui et al 2002), BSO can be efficiently used as an adjuvant for inactivated NDV vaccines especially in combination with the live vaccine.…”

Section: Discussionmentioning

confidence: 98%

“…Interestingly, recent evidences indicate that N. sativa oil, a potent anticancer, anti-inflammatory and antioxidant agent (Sharma et al 2009 ) could serve as an excellent adjuvant for a variety of viral pathogens. It is a non-specific immunomodulatory agent that profoundly enhances cytokine gene expression as well as cell-mediated and humoral immune responses (Umar et al 2015 ). To investigate its efficacy as an adjuvant for NDV vaccine, we prepared a W/O emulsion of our inactivated NDV in Nigella sativa oil and used in it in SPF chicken vaccination.…”

Section: Discussionmentioning

confidence: 99%

Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants

Aljumaili

Bello

Yeap

et al. 2020

Onderstepoort j. vet. res.

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Despite the availability of Newcastle disease (ND) vaccines for more than six decades, disease outbreaks continue to occur with huge economic consequences to the global poultry industry. The aim of this study is to develop a safe and effective inactivated vaccine based on a recently isolated Newcastle disease virus (NDV) strain IBS025/13 and evaluate its protective efficacy in chicken following challenge with a highly virulent genotype VII isolate. Firstly, high titre of IBS025/13 was exposed to various concentrations of binary ethylenimine (BEI) to determine the optimal conditions for complete inactivation of the virus. The inactivated virus was then prepared in form of a stable water-in-oil emulsion of black seed oil (BSO) or Freund’s incomplete adjuvant (FIA) and used as vaccines in specific pathogen-free chicken. Efficacy of various vaccine preparations was also evaluated based on the ability of the vaccine to protect against clinical disease, mortality and virus shedding following challenge with highly virulent genotype\VII NDV isolate. The results indicate that exposure of NDV IBS025/13 to 10 mM of BEI for 21 h at 37 °C could completely inactivate the virus without tempering with the structural integrity of the viral hemagglutin-neuraminidase protein. More so, the inactivated vaccines adjuvanted with either BSO- or FIA-induced high hemagglutination inhibition antibody titre that protected the vaccinated birds against clinical disease and in some cases virus shedding, especially when used together with live attenuated vaccines. Thus, genotype VII-based NDV-inactivated vaccines formulated in BSO could substantially improve poultry disease control particularly when combined with live attenuated vaccines.

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“…N. sativa has been shown to reduce the replication of influenza viruses by boosting T-helper, cytotoxic T, and bone marrow cellularity [69,70]. Moreover, N. sativa has been demonstrated to decrease proinflammatory cytokines, viral proteins (integrase, protease), and RNA polymerase II, all of which are essential for viral replication, and to raise splenocyte proliferation and interferon expression in a dosedependent manner.…”

Section: Discussionmentioning

confidence: 99%

Influence of Some Herbals on Immunostimulation of Cellular Immunity in Experimentally Ndv-Vaccinated Chicks

Zain Eldeen,

Younes,

El-bayoumi

et al. 2024

Egyptian Journal of Veterinary Sciences

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N EFFECTIVE substitute for the conventional virus control techniques used by the poultry business is the supplementation of herbal plants (cheap, naturally available and less toxic) in chicken diets. Application of them has been associated with notable improvements in the immunity and performance of birds. 180 cobb chicks were split into six equal-sized groups for the present study. A group A was left as a negative group, two groups (groups B and C) were left as control positive non-vaccinated infected group and control positive vaccinated infected group, respectively. Groups (groups D, E, and F) received an injection of vNDV strain associated with the ND Clone 30 vaccine and live LaSota vaccine and treated with medicinal herbs. The performance of the birds is enhanced by oral administration of Nigella sativa (6%), commercial curcumin® (1%), and Orego sol.®. All infected chicks secreted virus through trachea and cloaca, but the pattern differed among different groups. The chicks in group B showed more significant virus secretion. Similarly, increased expression of cytokine genes promotes immunostimulation action in different groups, leading to the reduction of NDV pathogenesis. When chicks were given Orego sol.®, there was a greater evident reduction in viral shedding and an improvement in their immune responses.

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RETRACTED: Effects of Nigella sativa on immune responses and pathogenesis of avian influenza (H9N2) virus in turkeys

Cited by 17 publications

References 25 publications

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

RETRACTED ARTICLE: Immunosuppressive interactions of viral diseases in poultry

Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants

Influence of Some Herbals on Immunostimulation of Cellular Immunity in Experimentally Ndv-Vaccinated Chicks

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