RETRACTED: Crosstalk between C/EBP homologous protein (CHOP) and glucocorticoid receptor in lung cancer

Mihailidou, Chrysovalantou; Panagiotou, Christina; Kiaris, Hippokratis; Kassi, Eva; Moutsatsou, Paraskevi

doi:10.1016/j.mce.2016.08.001

Cited by 21 publications

(22 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to increasing transgenic protein synthesis, attenuation of apoptosis by DEX-priming could also promote the observed increase in relative transgenic enzyme HAMANN ET AL. DEX has also been shown to modulate endoplasmic reticulum (ER) stress responses by promoting correct protein folding (Das et al, 2013), trafficking (Fujii et al, 2006), and by preventing apoptosis (Mihailidou, Panagiotou, Kiaris, Kassi, & Moutsatsou, 2016). DEX has also been shown to modulate endoplasmic reticulum (ER) stress responses by promoting correct protein folding (Das et al, 2013), trafficking (Fujii et al, 2006), and by preventing apoptosis (Mihailidou, Panagiotou, Kiaris, Kassi, & Moutsatsou, 2016).…”

Section: Dex Ameliorates Hmsc Protein Synthesis Inhibition Induced mentioning

confidence: 99%

Mechanisms of unprimed and dexamethasone‐primed nonviral gene delivery to human mesenchymal stem cells

Hamann

Broad

Nguyen

et al. 2018

Biotech & Bioengineering

View full text Add to dashboard Cite

Human mesenchymal stem cells (hMSCs) are under intense study for applications of cell and gene therapeutics because of their unique immunomodulatory and regenerative properties. Safe and efficient genetic modification of hMSCs could increase their clinical potential by allowing functional expression of therapeutic transgenes or control over behavior and differentiation. Viral gene delivery is efficient, but suffers from safety issues, while nonviral methods are safe, but highly inefficient, especially in hMSCs. Our lab previously demonstrated that priming cells before delivery of DNA complexes with dexamethasone (DEX), an anti‐inflammatory glucocorticoid drug, significantly increases hMSC transfection success. This work systematically investigates the mechanisms of hMSC transfection and DEX‐mediated enhancement of transfection. Our results show that hMSC transfection and its enhancement by DEX are decreased by inhibiting classical intracellular transport and nuclear import pathways, but DEX transfection priming does not increase cellular or nuclear internalization of plasmid DNA (pDNA). We also show that hMSC transgene expression is largely affected by pDNA promoter and enhancer sequence changes, but DEX‐mediated enhancement of transfection is unaffected by any pDNA sequence changes. Furthermore, DEX‐mediated transfection enhancement is not the result of increased transgene messenger RNA transcription or stability. However, DEX‐priming increases total protein synthesis by preventing hMSC apoptosis induced by transfection, resulting in increased translation of transgenic protein. DEX may also promote further enhancement of transgenic reporter enzyme activity by other downstream mechanisms. Mechanistic studies of nonviral gene delivery will inform future rationally designed technologies for safe and efficient genetic modification of clinically relevant cell types.

show abstract

Section: Dex Ameliorates Hmsc Protein Synthesis Inhibition Induced mentioning

confidence: 99%

Mechanisms of unprimed and dexamethasone‐primed nonviral gene delivery to human mesenchymal stem cells

Hamann

Broad

Nguyen

et al. 2018

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…High expression of GRα has been associated with indicators of less aggressive biological behavior in malignant neoplasms, such as smaller tumor size, early clinical stages, and higher survival rates of patients . These results have been attributed to the participation of GRα in immunoinflammatory responses, as well as cell proliferation and apoptosis . The mechanisms whereby GR modulates programmed cell death are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

“…5,10,11 These results have been attributed to the participation of GRα in immunoinflammatory responses, as well as cell proliferation and apoptosis. 8,12,13 The mechanisms whereby GR modulates programmed cell death are poorly understood. It has been shown that overexpression of GR triggers the death of tumor cells, 14,15 possibly by inhibiting the expression of the antiapoptotic proteins Bcl-2 and Bcl-xL.…”

Section: Introductionmentioning

confidence: 99%

Immunoexpression of glucocorticoid receptor alpha (GRα) isoform and apoptotic proteins (Bcl‐2 and Bax) in actinic cheilitis and lower lip squamous cell carcinoma

Sena

Silveira

Batista

et al. 2018

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…The net effect of these changes is increased energy and glucose metabolism. 97 Although mechanisms are still not clear as to how ER stress can modulate tumorigenesis, it has been reported that the CHOP-p21 axis may play a central role in connecting ER stress and a prosurvival phenotype in cancer, 98,99 as CHOP regulates p21 expression. It is thought that this may be predicated on the level of stress in the cell because CHOP-stimulated p21 expression is pronounced during low/moderate chronic stress.…”

Section: Abnormalities In Hbp and Er Stress In Cancermentioning

confidence: 99%

“…This suggests that in cancer the shift between UPR responses can go from being prosurvival to apoptotic and may be orchestrated by the cross talk in the UPR culminating in CHOP activation leading to p21 upregulation. 98,99 …”

Section: Abnormalities In Hbp and Er Stress In Cancermentioning

confidence: 99%

The Impact of the Unfolded Protein Response and the Hexosamine Biosynthetic Pathway on Glycosylation

2017

Glycobiology Insights

View full text Add to dashboard Cite

Glycan changes, in addition to being traditional mediators of protein quality control, cell signaling, and metabolism, are intimately linked to the progression of cancer and can potentially act as novel therapeutic and diagnostic targets. Despite advances in our understanding that glycosylation plays a key role in protein folding, maturation, and function, there is only limited understanding of the interconnected nature of glycan alterations to endoplasmic reticulum stress, the unfolded protein response, and the development of cancer. This review aims to highlight the interdependence of these pathways and the way they feed into each other to maintain protein quality control, regulate key survival mechanisms, and promote cell viability.

show abstract

RETRACTED: Crosstalk between C/EBP homologous protein (CHOP) and glucocorticoid receptor in lung cancer

Cited by 21 publications

References 48 publications

Mechanisms of unprimed and dexamethasone‐primed nonviral gene delivery to human mesenchymal stem cells

Mechanisms of unprimed and dexamethasone‐primed nonviral gene delivery to human mesenchymal stem cells

Immunoexpression of glucocorticoid receptor alpha (GRα) isoform and apoptotic proteins (Bcl‐2 and Bax) in actinic cheilitis and lower lip squamous cell carcinoma

The Impact of the Unfolded Protein Response and the Hexosamine Biosynthetic Pathway on Glycosylation

Contact Info

Product

Resources

About