RETRACTED: Chitosan tripolyphosphate (CS/TPP) nanoparticles: Preparation, characterization and application for gene delivery in shrimp

Vimal, S.; Majeed, Shahid; Taju, G.; Nambi, K.S.N.; Raj, N. Sundar; Madan, N.; Farook, M.A.; Rajkumar, T.; Gopinath, D.; Hameed, A.S. Sahul

doi:10.1016/j.actatropica.2013.07.013

Cited by 100 publications

(71 citation statements)

References 47 publications

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“…3b. For CS-TPP nanoparticles, a characteristic band at 3394 cm -1 is attributed to -NH 2 and -OH groups stretching vibration, 1636 (amide II), 1384 cm -1 (C 3 -O) and 1073 cm -1 (C 6 -O) as reported earlier (Vimal et al 2013). For FA/CS-TPP NPs, increased peak intensity at each bond stretching resulted in shifting of peak values.…”

Section: Fourier Transformation Infrared Spectroscopy Analysismentioning

confidence: 68%

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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Ferulic acid (FA) is a widely distributed hydroxycinnamic acid found in various cereals and fruits exhibiting potent antioxidant and anticancer activities. However, due to low solubility and permeability, its availability to biological systems is limited. Non-toxic chitosan-tripolyphosphate pentasodium (CS-TPP) nanoparticles (NPs) are used to load sparingly soluble molecules and drugs, increasing their bioavailability. In the present work, we have encapsulated FA into the CS-TPP NPs to increase its potential as a therapeutic agent. Different concentrations of FA were tested to obtain optimum sized FA-loaded CS-TPP nanoparticles (FA/CS-TPP NPs) by ionic gelation method. Nanoparticles were characterized by scanning electron microscopy, Fourier transformation infrared spectroscopy (FTIR), thermogravimetric analyses and evaluated for their anticancer activity against ME-180 human cervical cancer cell lines. The FTIR spectra confirmed the encapsulation of FA and thermal analysis depicted its degradation profile. A concentration-dependent relationship between FA encapsulation efficiency and FA/ CS-TPP NPs diameter was observed. Smooth and spherical FA-loaded cytocompatible nanoparticles with an average diameter of 125 nm were obtained at 40 lM FA conc. The cytotoxicity of 40 lM FA/CS-TPP NPs against ME-180 cervical cancer cell lines was found to be higher as compared to 40 lM native FA. Apoptotic morphological changes as cytoplasmic remnants and damaged wrinkled cells in ME-180 cells were visualized using scanning electron microscopic and fluorescent microscopic techniques. Data concluded that chitosan enveloped FA nanoparticles could be exploited as an excellent therapeutic drug against cancer cells proliferation.

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Section: Fourier Transformation Infrared Spectroscopy Analysismentioning

confidence: 68%

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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“…The recently introduced ISAV vaccine from Centrovet uses a proprietary microencapsulation system designed to protect the SVP-based ISAV hemagglutinin esterase antigen from destruction [104]. While the composition of this encapsulation matrix was not disclosed by these authors, other research has been on-going using materials such as alginate and chitosan to improve oral delivery of vaccines in fish [100][101][102]107] and work on this area continues.…”

Section: Encapsulation Of Oral Vaccinesmentioning

confidence: 99%

Viral vaccines for farmed finfish

2013

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Over the past decade, aquaculture has grown at an average annual growth rate of approximately 6 % worldwide despite many challenges. Viral diseases are one of the major challenges that are threatening a sustainable growth of finfish farming globally. Vaccination of farmed fish plays an important role in commercial fish farming to mitigate viral diseases. In this review, we summarized the major viral diseases that have caused serious economic losses, and emerging diseases that pose a potential threat to aquaculture. The current status of viral vaccines in farmed fish are discussed, particularly the different types of vaccines that were licensed in recent years and are now commercially available, and the routes of delivery of those vaccines including the merits and demerits of each of these delivery method. Furthermore, the article provides an overview of different experimental vaccines that have been reported in the literatures in recent years besides highlighting the future need for developing cost-effective, oral vaccines that can be easily applicable at farm level.

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“…Due to this modification can improve biodistribution and increase specificity and sensitivity, and reduced pharmacological toxicity. 17 The topography, surfaces, structures, morphologies, and composition of the chitosan nanoparticles were studied using Scanning Electron Microscopic (SEM). 18 The SEM micrograph obtained for the pure chitosan revealed that the texture is plain without pores having smooth, compact and homogeneous even surface structure with no gross effects.…”

Section: Tuberculosis Is a Chronic Disease Caused By Mycobacterium Tumentioning

confidence: 99%

“…17 Initially in order to create the homogeneous chitosan solution, about 1.5 g of chitosan dissolved in 200 ml of 2% acetic acid solution was kept under magnetic stirring process for about 20 min. Then to the above prepared chitosan solution, 0.8 g of sodium tripolyphosphate dissolved in 107 ml of conductivity water was added drop wise and stirred well for about 30 min to reach equilibrium.…”

Section: Preparation Of Chitosan Nanoparticlesmentioning

confidence: 99%

In vitro Antibacterial Activity of Chitosan Nanoparticles against Mycobacterium tuberculosis

Sudjarwo¹,

Mahmiah²,

Sudjarwo³

2017

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Background:Chitosan nanoparticles have gained growing interest for nanomedicine, biomedical engineering and development of new therapeutic with improved bioavailability, increased sensitivity and specificity, and reduced toxicity. Objective: The aim of the present study is to synthesis of the chitosan nanoparticles for antimycobacterial applications. Methods: Chitosan were isolated from the shrimp shell. Tripolyphosphate (TPP) will be used to prepare chitosan nanoparticles by ionotropic gelation method. The size and morphology of the chitosan nanoparticle was analyzed by scanning electron microscope (SEM). The broth microdilution method is used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of nanoparticle chitosan on strain M. tuberculosis H37Rv. Results: The SEM micrographs of the nano-magnetic chitosan showed that they were approximately uniform spheres and the rough surface morphology, have a solid dense cubical or rectangular structure. Using the broth microdilution susceptibility method, chitosan nanoparticle was found to have the antimycobacterial effects with a MIC value of 1200 μg/mL whilst MBCs value of 2400 μg/mL for M. tuberculosis H37Rv. Conclusion: The conclusion from the study was chitosan nanoparticle have potential as a source of lead compounds that may be developed further into antimycobacterial drugs.

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RETRACTED: Chitosan tripolyphosphate (CS/TPP) nanoparticles: Preparation, characterization and application for gene delivery in shrimp

Cited by 100 publications

References 47 publications

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

Viral vaccines for farmed finfish

In vitro Antibacterial Activity of Chitosan Nanoparticles against Mycobacterium tuberculosis

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