RETRACTED: Cell Adhesion-Dependent Control of MicroRNA Decay

Kim, Young‐Kook; Yeo, Jinah; Ha, Minju; Kim, Boseon; Kim, V. Narry

doi:10.1016/j.molcel.2011.07.031

Cited by 33 publications

(33 citation statements)

References 31 publications

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“…Recent studies indicated that the stability of some miRNAs is based on cell conditions and gene expression in animals. 49,50 If we can isolate P-bodies and then miRNAs in P-bodies, it may explain the fact that only a subset of miRNAs were reduced in decapping mutants.…”

Section: Discussionmentioning

confidence: 99%

The role of decapping proteins in the miRNA accumulation inArabidopsis thaliana

Motomura

Kumakura

et al. 2012

RNA Biology

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Section: Discussionmentioning

confidence: 99%

The role of decapping proteins in the miRNA accumulation inArabidopsis thaliana

Motomura

Kumakura

et al. 2012

RNA Biology

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“…Sequences near the 3′ end of miRNAs have been shown to affect miRNA stability [71]; this is not surprising since 3′ modification/degradation seems to be a major mode of miRNA turnover such that 3′ sequences may affect the accessibility of the decay machinery to the miRNAs. Elements in the middle of the small RNA as well as the overall percentage of AU or UA dinucleotides also serve as destabilization signals [72][73][74]. However, the enzymes responsible for sequence-specific decay of small RNAs have not been identified in most cases.…”

Section: Target Rnas Affect the Stability Of Small Rnasmentioning

confidence: 99%

Regulation of small RNA stability: methylation and beyond

2012

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As central components of RNA silencing, small RNAs play diverse and important roles in many biological processes in eukaryotes. Aberrant reduction or elevation in the levels of small RNAs is associated with many developmental and physiological defects. The in vivo levels of small RNAs are precisely regulated through modulating the rates of their biogenesis and turnover. 2′-O-methylation on the 3′ terminal ribose is a major mechanism that increases the stability of small RNAs. The small RNA methyltransferase HUA ENHANCER1 (HEN1) and its homologs methylate microRNAs and small interfering RNAs (siRNAs) in plants, Piwi-interacting RNAs (piRNAs) in animals, and siRNAs in Drosophila. 3′ nucleotide addition, especially uridylation, and 3′-5′ exonucleolytic degradation are major mechanisms that turnover small RNAs. Other mechanisms impacting small RNA stability include complementary RNAs, cis-elements in small RNA sequences and RNA-binding proteins. Investigations are ongoing to further understand how small RNA stability impacts their accumulation in vivo in order to improve the utilization of RNA silencing in biotechnology and therapeutic applications.

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“…Relevantly, even with 10-fold lower levels of Renilla expression, the miR-Sens system is still reliable, allowing, for instance, the incorporation of destabilized Renilla, which is expressed at lower steady-state levels, but which will show a more rapid response to translational repression after induction of miRNA activity. This modification should offer new perspectives to the study of the tightly regulated miRNA turnover as reported for miR-122 or miR-141 (Katoh et al 2009;Kim et al 2011). Likewise, given the reported payload of pMSCV (Baldeschi et al 2003), we expect that 39 UTRs as long as 5 kb can be cloned in the miR-Sens vector and tested in target cells without loss of sensitivity.…”

Section: Discussionmentioning

confidence: 88%

miR-Sens—a retroviral dual-luciferase reporter to detect microRNA activity in primary cells

Beillard¹,

Ong²,

Giannakakis³

et al. 2012

RNA

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MicroRNA-mRNA interactions are commonly validated and deconstructed in cell lines transfected with luciferase reporters. However, due to cell type-specific variations in microRNA or RNA-binding protein abundance, such assays may not reliably reflect microRNA activity in other cell types that are less easily transfected. In order to measure miRNA activity in primary cells, we constructed miR-Sens, a MSCV-based retroviral vector that encodes both a Renilla luciferase reporter gene controlled by microRNA binding sites in its 39 UTR and a Firefly luciferase normalization gene. miR-Sens sensors can be efficiently transduced in primary cells such as human fibroblasts and mammary epithelial cells, and allow the detection of overexpressed and, more importantly, endogenous microRNAs. Notably, we find that the relative luciferase activity is correlated to the miRNA expression, allowing quantitative measurement of microRNA activity. We have subsequently validated the miR-Sens 39 UTR vectors with known human miRNA-372, miRNA-373, and miRNA-31 targets (LATS2 and TXNIP). Overall, we observe that miRSens-based assays are highly reproducible, allowing detection of the independent contribution of multiple microRNAs to 39 UTR-mediated translational control of LATS2. In conclusion, miR-Sens is a new tool for the efficient study of microRNA activity in primary cells or panels of cell lines. This vector will not only be useful for studies on microRNA biology, but also more broadly on other factors influencing the translation of mRNAs.

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RETRACTED: Cell Adhesion-Dependent Control of MicroRNA Decay

Cited by 33 publications

References 31 publications

The role of decapping proteins in the miRNA accumulation inArabidopsis thaliana

The role of decapping proteins in the miRNA accumulation inArabidopsis thaliana

Regulation of small RNA stability: methylation and beyond

miR-Sens—a retroviral dual-luciferase reporter to detect microRNA activity in primary cells

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