RETRACTED ARTICLE: Silencing of long non-coding RNA MALAT1 suppresses inflammation in septic mice: role of microRNA-23a in the down-regulation of MCEMP1 expression

Xie, Wenli; Chen, Lei; Chen, Li; Kou, Qiuye

doi:10.1007/s00011-019-01306-z

Cited by 26 publications

(18 citation statements)

References 31 publications

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“…However, the results of our nuclear-cytoplasmic RNA fractionation assays indicated that MALAT1 was mainly located in the cytoplasm of MCF-7, MDA-MB-231, A549, and H1299 cells, under either normoxia or hypoxia ( Figure 3 ). These results are consistent with recent studies showing that MALAT1 was located in the cytoplasm in human hepatocellular carcinoma cells, monocytes, and human pulmonary microvascular endothelial cells ( 29 , 30 , 35 ). Therefore, the labeling of MALAT1 as a nuclear marker should be done with the caveat that this status is dependent on the cell type.…”

Section: Discussionsupporting

confidence: 93%

“…Many studies have reported that miRNAs are differentially expressed in hypoxia and related to various aspects of cancer pathogenesis and progression, such as cell differentiation, proliferation, migration, invasion, apoptosis, and drug resistance (22)(23)(24)(25)(26)(27)(28). Some studies have reported the interaction between lncRNA and miRNA, specifically that lncRNA can be competing endogenous RNA by acting as a sponge for miRNA (29,30).…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p

et al. 2021

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Hypoxia, a common process during tumor growth, can lead to tumor aggressiveness and is tightly associated with poor prognosis. Long noncoding RNAs (lncRNAs) are long ribonucleotides (>200 bases) with limited ability to translate proteins, and are known to affect many aspects of cellular function. One of their regulatory mechanisms is to function as a sponge for microRNA (miRNA) to modulate its biological functions. Previously, MALAT1 was identified as a hypoxia-induced lncRNA. However, the regulatory mechanism and functions of MALAT1 in breast cancer are still unclear. Therefore, we explored whether MALAT1 can regulate the functions of breast cancer cells through miRNAs. Our results showed the expression levels of MALAT1 were significantly up-regulated under hypoxia and regulated by HIF-1α and HIF-2α. Next, in contrast to previous reports, nuclear and cytoplasmic fractionation assays and fluorescence in situ hybridization indicated that MALAT1 was mainly located in the cytoplasm. Therefore, the labeling of MALAT1 as a nuclear marker should be done with the caveat. Furthermore, expression levels of miRNAs and RNA immunoprecipitation using antibody against AGO2 showed that MALAT1 functioned as a sponge of miRNA miR-3064-5p. Lastly, functional assays revealed that MALAT1 could promote cellular migration and proliferation of breast cancer cells. Our findings provide evidence that hypoxia-responsive long non-coding MALAT1 could be transcriptionally activated by HIF-1α and HIF-2α, act as a miRNA sponge of miR-3064-5p, and promote tumor growth and migration in breast cancer cells. These data suggest that MALAT1 may be a candidate for therapeutic targeting of breast cancer progression.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p

et al. 2021

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“…The MCEMP1 gene encodes mast cellexpressed membrane protein 1 and can be expressed in mast cells, macrophages and even the brain tissue (53,54). The effect of MCEMP1 on QIVs-induced immunity in elderly populations remains to be elucidated, although growing studies demonstrated its diagnostic capacity on the progression of stroke (53), and the promotion effect of its high expression on inflammation and sepsis (55).…”

Section: Discussionmentioning

confidence: 99%

Identifying Potential Candidate Hub Genes and Functionally Enriched Pathways in the Immune Responses to Quadrivalent Inactivated Influenza Vaccines in the Elderly Through Co-Expression Network Analysis

et al. 2020

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Insights into the potential candidate hub genes may facilitate the generation of safe and effective immunity against seasonal influenza as well as the development of personalized influenza vaccines for the elderly at high risk of influenza virus infection. This study aimed to identify the potential hub genes related to the immune induction process of the 2018/19 seasonal quadrivalent inactivated influenza vaccines (QIVs) in the elderly ≥60 years by using weighted gene co-expression network analysis (WGCNA). From 63 whole blood samples from16 elderly individuals, a total of 13,345 genes were obtained and divided into eight co-expression modules, with two modules being significantly correlated with vaccine-induced immune responses. After functional enrichment analysis, genes under GO terms of vaccine-associated immunity were used to construct the sub-network for identification and functional validation of hub genes. MCEMP1 and SPARC were confirmed as the hub genes with an obvious effect on QIVs-induced immunity. The MCEMP1 expression was shown to be negatively correlated with the QIVs-associated reactogenicity within 7 days after vaccination, which could be suppressed by the CXCL 8/IL-8 and exacerbated by the Granzyme-B cytotoxic mediator. Meanwhile, the SPARC expression was found to increase the immune responses to the QIVs and contribute to the persistence of protective humoral antibody titers. These two genes can be used to predict QIVs-induced adverse reaction, the intensity of immune responses, and the persistence of humoral antibody against influenza. This work has shed light on further research on the development of personalized QIVs with appropriate immune responses and long-lasting immunity against the forthcoming seasonal influenza.

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“…lncRNA HOTAIR promoted the progression of sepsis, by inhibiting monocyte proliferation, while promoting monocyte apoptosis and inflammation response; this was achieved by acting as miR-211 sponge, thereby inducing IL-6R expression in CLP-induced sepsis mice model ( Chen et al., 2019 ). lncRNA MALAT1 also promoted inflammation and proliferation of monocytes, while inhibiting monocyte apoptosis in CLP-induced septic mice; these effects, at the molecular level, were mediated through the binding of lncRNA MALAT1 to miR-23a and up-regulated MCEMP1 through loss- and gain-of-function experiments in vivo ( Xie et al., 2020 ). Another study confirmed that lncRNA NEAT1 promoted the inflammation and apoptosis while restraining the proliferation of LPS-induced macrophages via the miR-370-3p/TSP-1 axis ( Wu et al., 2020 ).…”

Section: Role Of Lncrnas In Sepsismentioning

confidence: 99%

Long Noncoding RNA: Regulatory Mechanisms and Therapeutic Potential in Sepsis

Wang

Yang

Wen

et al. 2021

Front. Cell. Infect. Microbiol.

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Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection and is characterized by a hyperinflammatory state accompanied by immunosuppression. Long noncoding RNAs (lncRNAs) are noncoding RNAs longer than 200 nucleotides and have important roles in mediating various biological processes. Recently, lncRNAs were found to exert both promotive and inhibitory immune functions in sepsis, thus participating in sepsis regulation. Additionally, several studies have revealed that lncRNAs are involved in sepsis-induced organ dysfunctions, including cardiovascular dysfunction, acute lung injury, and acute kidney injury. Considering the lack of effective biomarkers for early identification and specific treatment for sepsis, lncRNAs may be promising biomarkers and even targets for sepsis therapies. This review systematically highlights the recent advances regarding the roles of lncRNAs in sepsis and sheds light on their use as potential biomarkers and treatment targets for sepsis.

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RETRACTED ARTICLE: Silencing of long non-coding RNA MALAT1 suppresses inflammation in septic mice: role of microRNA-23a in the down-regulation of MCEMP1 expression

Cited by 26 publications

References 31 publications

Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p

Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p

Identifying Potential Candidate Hub Genes and Functionally Enriched Pathways in the Immune Responses to Quadrivalent Inactivated Influenza Vaccines in the Elderly Through Co-Expression Network Analysis

Long Noncoding RNA: Regulatory Mechanisms and Therapeutic Potential in Sepsis

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