RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis

Zhang, Tao; Mudgett, Michael; Rambabu, R.; Abramson, Bradley W.; Dai, Xinhua; Michael, Todd P.; Zhao, Yunde

doi:10.1038/s41467-021-24195-5

Cited by 19 publications

(19 citation statements)

References 22 publications

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“…In a previous study, we have demonstrated that induction of DSBs using CRISPR-Cas9 can lead to up to 14% of inter-homolog recombination (IHR) in somatic cells at the Psy1 locus of tomato [23]. In Arabidopsis, a recent study also showed that IHR can be used for DSB repair, generating gene conversion, or a gene drive [24]. This was shown in a transgenic system and IHR-mediated DSB repair has not been analyzed yet for endogenous loci in Arabisopsis.…”

Section: Of 15mentioning

confidence: 99%

Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin

Filler-Hayut

Kniazev

Melamed-Bessudo

et al. 2021

IJMS

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Homologous recombination (HR) typically occurs during meiosis between homologs, at a few unplanned locations along the chromosomes. In this study, we tested whether targeted recombination between homologous chromosomes can be achieved via Clustered Regulatory Interspaced Short Palindromic Repeat associated protein Cas9 (CRISPR-Cas9)-induced DNA double-strand break (DSB) repair in Arabidopsis thaliana. Our experimental system includes targets for DSB induction in euchromatic and heterochromatic genomic regions of hybrid F1 plants, in one or both parental chromosomes, using phenotypic and molecular markers to measure Non-Homologous End Joining and HR repair. We present a series of evidence showing that targeted DSBs can be repaired via HR using a homologous chromosome as the template in various chromatin contexts including in pericentric regions. Targeted crossover was rare, but gene conversion events were the most frequent outcome of HR and were found in both “hot and cold” regions. The length of the conversion tracts was variable, ranging from 5 to 7505 bp. In addition, a typical feature of these tracks was that they often were interrupted. Our findings pave the way for the use of targeted gene-conversion for precise breeding.

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Section: Of 15mentioning

confidence: 99%

Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin

Filler-Hayut

Kniazev

Melamed-Bessudo

et al. 2021

IJMS

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show abstract

“…Though the most rapid progress has been in Anopheles mosquitoes for reduction of vector-borne disease [5][6][7] (for which modification drives have also been developed [8][9][10]), suppression gene drives could also be used to remove invasive species or agricultural pests. Thus far, gene drives have been demonstrated in a variety of organisms, including yeast [11,12], flies [13][14][15][16][17][18], mice [19] and plants [20]. Most of these have been homing type drives, where a nuclease, usually CRISPR/Cas9, cuts a wild-type chromosome at a site-directed by its guide RNA (gRNA) [21].…”

Section: Introductionmentioning

confidence: 99%

Modelling homing suppression gene drive in haplodiploid organisms

Liu

Champer

2022

Proc. R. Soc. B.

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Gene drives have shown great promise for suppression of pest populations. These engineered alleles can function by a variety of mechanisms, but the most common is the CRISPR homing drive, which converts wild-type alleles to drive alleles in the germline of heterozygotes. Some potential target species are haplodiploid, in which males develop from unfertilized eggs and thus have only one copy of each chromosome. This prevents drive conversion, a substantial disadvantage compared to diploids where drive conversion can take place in both sexes. Here, we study homing suppression gene drives in haplodiploids and find that a drive targeting a female fertility gene could still be successful. However, such drives are less powerful than in diploids and suffer more from functional resistance alleles. They are substantially more vulnerable to high resistance allele formation in the embryo owing to maternally deposited Cas9 and guide RNA and also to somatic cleavage activity. Examining spatial models where organisms move over a continuous landscape, we find that haplodiploid suppression drives surprisingly perform nearly as well as in diploids, possibly owing to their ability to spread further before inducing strong suppression. Together, these results indicate that gene drive can potentially be used to effectively suppress haplodiploid populations.

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“…Though the most rapid progress has been in Anopheles mosquitoes for reduction of vector-borne disease 5–7 (for which modification drives have also been developed 8–10 ), suppression gene drives could also be used to remove invasive species or agricultural pests. Thus far, gene drives have been demonstrated in a variety of organisms, including yeast 11,12 , flies 13–1516–18 , mice 19 , and plants 20 . Most of these have been homing types drives, where a nuclease, usually CRISPR/Cas9, cuts a wild-type chromosome at a site directed by its guide RNA (gRNA) 21 .…”

Section: Introductionmentioning

confidence: 99%

Modelling homing suppression gene drive in haplodiploid organisms

Liu

Champer

2021

Preprint

View full text Add to dashboard Cite

Gene drives have shown great promise for suppression of pest populations. These engineered alleles can function by a variety of mechanisms, but the most common is the CRISPR homing drive, which converts wild-type alleles to drive alleles in the germline of heterozygotes. Some potential target species are haplodiploid, in which males develop from unfertilized eggs and thus have only one copy of each chromosome. This prevents drive conversion, a substantial disadvantage compared to diploids where drive conversion can take place in both sexes. Here, we study the characteristics of homing suppression gene drives in haplodiploids and find that a drive targeting a female fertility gene could still be successful. However, such drives are less powerful than in diploids. They are substantially more vulnerable to high resistance allele formation in the embryo due to maternally deposited Cas9 and gRNA and also to somatic cleavage activity. Examining models of continuous space where organisms move over a landscape, we find that haplodiploid suppression drives surprisingly perform nearly as well as in diploids, possibly due to their ability to spread further before inducing strong suppression. Together, these results indicate that gene drive can potentially be used to effectively suppress haplodiploid populations.

show abstract

RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis

Cited by 19 publications

References 22 publications

Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin

Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin

Modelling homing suppression gene drive in haplodiploid organisms

Modelling homing suppression gene drive in haplodiploid organisms

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