RETRACTED ARTICLE: Potential Start Codon Targeted (SCoT) and Inter-retrotransposon Amplified Polymorphism (IRAP) Markers for Evaluation of Genetic Diversity and Conservation of Wild Pistacia Species Population

Abstract

Wild pistachio species is important species in forests regions Iran and provide protection wind and soil erosion. Even though cultivation and utilization of Pistacia are fully exploited, the evolutionary history of the Pistacia genus and the relationships among the species and accessions is still not well understood. Two molecular marker strategies, SCoT and IRAP markers were analyzed for assessment of 50 accessions of this species accumulated from diverse geographical areas of Iran. A thorough of 115 bands were amplified using eight IRAP primers, of which 104 (90.4 %) have been polymorphic, and 246 polymorphic bands (68.7 %) had been located in 358 bands amplified by way of forty-four SCoT primers. Average PIC for IRAP and SCoT markers became 0.32 and 0.48, respectively. This is exposed that SCoT markers have been extra informative than IRAP for the assessment of variety among pistachio accessions. Primarily based on the two extraordinary molecular markers, cluster evaluation revealed that the 50 accessions taken for the evaluation may be divided into three distinct clusters. Those results recommend that the performance of SCoT and IRAP markers was highly the equal in fingerprinting of accessions. The results affirmed a low genetic differentiation among populations, indicating the opportunity of gene drift most of the studied populations. These findings might render striking information in breeding management strategies for genetic conservation and cultivar improvement.

RETRACTED ARTICLE: Comparison of traditional and new generation DNA markers declares high genetic diversity and differentiated population structure of wild almond species

“…Population structure was analysed using a model-based approach, Bayesian method, in STRUCTURE ver.3.0 software 72 . Since dominant markers were used in this study, each class of the accessions of wild Prunus species was treated as a haploid allele 73 . Model-based cluster analysis was used to test the number of populations (K).…”

Genetic diversity of Bletilla striata assessed by SCoT and IRAP markers

“…Karim Sorkheh et al used IRAP for germplasm analysis of wild Pistacia chinensis [16], and Ahmad Mousapour Gorji et al used SCoT to analyze the genetic fingerprint of tetraploid potatoes [1]. We used 20 μL of the IRAP and SCoT molecular markers in both studies.…”

Landscape Population Genomics of Forsythia (Forsythia suspensa) Reveal That Ecological Habitats Determine the Adaptive Evolution of Species

“…Start codon targeted (SCoT) polymorphism is a gene-targeted marker, which was developed based on short conserved start codon in plant genes (Collard and Mackill, 2009). SCoT markers are highly variable and reproducible and have been widely used to survey population genetics and phylogenetics (Guo et al, 2014; Feng et al, 2015; Sorkheh et al, 2016). However, SCoT markers have been seldom used in landscape population genomics to detect adaptive loci in genomes.…”