RETRACTED ARTICLE: MicroRNA-183 affects the development of gastric cancer by regulating autophagy via MALAT1-miR-183-SIRT1 axis and PI3K/AKT/mTOR signals

Abstract

Gastric cancer (GC) remains to be a familiar malignant tumor with poor prognosis and daunting impacts on global health. We planned to grab the latent impacts of microRNA-183 in regulating cell autophagy, thus to clarify its possible regulatory principle in GC. The miR-183 level in GC tissues and cell lines was investigated. The impacts of miR-183 dysregulation on cell biological performances including viability, apoptosis and autophagy of GC cell lines including SGC-7901 were detected. Also, cells were disposed with 3-methyladenine (3-MA, an autophagy inhibition) before dysregulation of miR-183 to further investigate the correlation between cell autophagy and viability or apoptosis. Furthermore, the regulatory mechanisms between miR-183 and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), silent mating type information regulation 1 (SIRT1) or PI3K/AKT/mTOR pathway were explored. miR-183 was under-expressed both in GC tissues and in cell lines. miR-183 mimic alone depressed SGC-7901 cell viability and enhanced cell apoptosis and autophagy, whereas miR-183 inhibitor exhibited opposite effects. Moreover, the impacts of miR-183 on SGC-7901 cell viability and apoptosis were mediated by affecting the activation of autophagy. Our results indicate that miR-183 is under-expressed in GC cells and depression of miR-183 may enhance GC cell viability and inhibit cell apoptosis by affecting the activation of cell autophagy. MALAT1-miR-183-SIRT1 axis and PI3K/AKT/mTOR pathway may be mechanisms to mediate autophagy in GC. miR-183 may serve as a towardly therapeutic target for GC. ARTICLE HISTORY

<p>Regulatory Networks of LncRNA MALAT-1 in Cancer</p>

“…142 Activation of the MALAT-1/miR-202/Gli2 axis is correlated with clinical features of GC, 143 and GC cell viability, apoptosis, and autophagy are partly regulated by MALAT-1/miR-183/SIRT1 and MALAT-1/miR-183/PI3K/AKT/mTOR signaling. 144 By sponging miR-1297, MALAT-1 promotes GC progression by upregulating High-mobility group box (HMGB) 2, which is involved in chemoresistance in GC. 145,146 The MALAT-1/miR-23b-3p axis confers chemoresistance by inducing prosurvival autophagy.…”

<p>miR-939-5p Contributes to the Migration and Invasion of Pancreatic Cancer by Targeting ARHGAP4</p>

“…12,13 Nevertheless, the regulation of ARHGAP4 in pancreatic cancer is still undiscovered microRNAs (miRNA) are small endogenous non-coding RNA molecules with a length of about 18-25 nucleotides that repress protein translation through binding to the 3ʹ-untranlated region (UTR) of their target mRNA and are significantly involved in various cancers, including pancreatic cancer. [14][15][16] High expression of miR-196a, miR-27a, miR-221, miR-143, miR-135b, miR-199b-5p and miR-21, but low expression of miR-744, miR-455-3p and miR-655, in pancreatic cancer samples were found compared with normal samples and associated with poor prognosis. 17 In addition, the expression profile of miRNAs varies even in the different locations, 18 which further confirms that miRNAs have high application value in the diagnosis, prognosis evaluation and treatment of pancreatic cancer.…”

Integrated Analysis of a circRNA-miRNA-mRNA Axis in Intervertebral Disc Degeneration

“…The regulated network of hsa_circ_0091570-hsa-miR-508-5p-CHEK1/TSGA10/GSTCD was constructed and the RNAs (circRNAs and miRNAs) and genes in the network may all be novel targets for IDD. In parallel, Li et al reported that miR-183-3p regulates autophagy in gastric cancer cells by the PI3K/AKT/mTOR pathway [37]. According to previous research, the PI3K/AKT/mTOR pathway has a signi cant effect on cell growth, survival, metabolism, proliferation, and angiogenesis [38].…”