RETRACTED ARTICLE: Long non-coding RNA SNHG16 reduces hydrogen peroxide-induced cell injury in PC-12 cells by up-regulating microRNA-423-5p

Abstract

Functions of long non-coding RNAs (lncRNAs) have been widely probed in spinal cord injury (SCI). But, the influences of lncRNA-small nucleolar RNA host gene 16 (lncRNA-SNHG16) is still not well documented in SCI. The study explored the impacts of SNHG16 on H 2 O 2-injured PC-12 cells. PC-12 cells were disposed with H 2 O 2 , cell viability, apoptosis, autophagy and ROS level were detected. RT-qPCR was executed to explore SNHG16 or miR-423-5p expression in H 2 O 2-stimulated cells. After transfection with pc-SNHG16 or miR-423-5p inhibitor, the functions of SNHG16 and miR-423-5p in H 2 O 2-injured cells were studied. AMPK and ERK1/2 pathways were finally assessed by western blot. We found that H 2 O 2 evoked cell injury in PC-12 cells, and repressed SNHG16 was observed in H 2 O 2-disposed cells. Overexpressed SNHG16 prominently alleviated H 2 O 2-induced cell injury as indicated by repressing cell apoptosis, autophagy and ROS level. Additionally, SNGH16 enhanced miR-423-5p expression, and miR-423-5p inhibition abrogated the protective effect of SNGH16 on H 2 O 2-injured PC-12 cells. SNGH16 mediated AMPK and ERK1/2 pathways via up-regulating miR-423-5p in H 2 O 2-injured PC-12 cells. In conclusion, these findings indicated that SNGH16 reduced H 2 O 2-evoked cell injury by mediating miR-423-5p in PC-12 cells. The findings might uncover the effect of SNHG16 on SCI, which provide a new reference for remedying SCI. HIGHLIGHTS 1. H 2 O 2 evokes cell injury in PC-12 cells; 2. SNHG16 reduces H 2 O 2-induced cell injury in PC-12 cells; 3. SNGH16 enhances miR-423-5p expression in H 2 O 2-stimulated PC-12 cells; 4. MiR-423-5p inhibition abrogates the protective effect of SNGH16 in PC-12 cells; 5. SNGH16 mediates AMPK and ERK1/2 pathways by up-regulating miR-423-5p.

RETRACTED ARTICLE: Overexpression of lncRNA ANRIL aggravated hydrogen peroxide-disposed injury in PC-12 cells via inhibiting miR-499a/PDCD4 axis-mediated PI3K/Akt/mTOR/p70S6K pathway

“…In very recent studies, lncRNA SNHG5 has been shown to promote astrocytes and microglia viability in SCI [33]; and lncRNA ZNF667-AS1 impacts potential function in promoting recovery of SCI [34]. Moreover, lncRNA SNGH16 is shown to reduce H 2 O 2 -disposed cell injury in PC-12 cells, providing a new reference for remedying SCI [35]. These data suggest that lncRNAs may be pivotal factors in SCI.…”

Dexmedetomidine had neuroprotective effects on hippocampal neuronal cells via targeting lncRNA SHNG16 mediated microRNA-10b-5p/BDNF axis

“…Similarly, SNHG16 is also a member of the lncRNA molecule, and several studies have indicated that it can promote the progression of malignant tumors. Furthermore, SNHG16 reverses LPS-induced inflammatory response in neurosepsis [29] and also attenuates hydrogen peroxide-induced PC12 neuronal damage [30]. In the present study, we found that the neuroprotective effect of DEX was closely related to the upregulated expression of SNHG16, while the neuroprotective effect of DEX on OGD-induced neuron injury was significantly decreased after knocking down SNHG16.…”

Differential Circular RNA Expression Profiles Following Spinal Cord Injury in Rats: A Temporal and Experimental Analysis

“…Previous studies have shown that upregulation of miRNA-423-5p significantly decreases cell proliferation and migration (Ge et al, 2018;Guo et al, 2018). In a very recently published study, Liu et al demonstrated that promoting miR-423-5p expression reduces hydrogen peroxide-induced PC-12 cell injury (Liu et al, 2019). We speculate that miR-423-5p upregulation, which was induced by a reduction in the level of circRNA_01477 following hemisection SCI in rats, affects regeneration by inhibiting cell proliferation and migration.…”