RETRACTED ARTICLE: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Shi, Juanjuan; Xu, Xijian; Zhang, Dan; Zhang, Jiuyan; Yang, Hui; Li, Chang; Li, Rui; Xuan, Wei; Luan, Wenqing; Liu, Peishu

doi:10.1186/s13048-020-00723-7

Cited by 16 publications

(15 citation statements)

References 49 publications

(27 reference statements)

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“…In the circ_0067934-interacted miRNAs in bioinformatics analysis (CircInteractome online database), we found that miR-545-3p has presented tumor suppressor function in various cancers, such as liver cancer, lung cancer, and ovarian cancer ( 15 – 17 ), but the function of miR-545-3p has not been reported. Meanwhile, we identified that circ_0067934 inhibited ferroptosis of thyroid cancer cells and miR-545-3p potentially targeted ferroptosis-regulator SLC7A11 in a bioinformatics analysis (ENCORI online database).…”

Section: Resultsmentioning

confidence: 98%

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Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling

Zhan

2021

Front. Endocrinol.

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Ferroptosis is an emerging programmed cell death distinguished from apoptosis and autophagy and plays essential roles in tumorigenesis. Thyroid cancer is a prevalent endocrine tumor, but the molecular mechanism of ferroptosis during thyroid cancer development remains unclear. Here, we identified the critical function of circular RNA circ_0067934 in repressing ferroptosis of thyroid cancer cells. Our data showed that the ferroptosis activator erastin decreased thyroid cancer cell viabilities, while the circ_0067934 shRNA further attenuated erastin-inhibited cell viabilities. The silencing of circ_0067934 enhanced the levels of ferroptosis-related markers, including Fe2+, iron, and ROS in the cells. The knockdown of circ_0067934 induced thyroid cancer cell apoptosis and repressed thyroid cancer cell proliferation in vitro and in vivo. Circ_0067934 upregulated the expression of the ferroptosis-negative regulator SLC7A11 by sponging and inhibiting miR-545-3p in thyroid cancer cells. The overexpression of SLC7A11 or the inhibitor of miR-545-3p reversed circ_0067934 silencing-regulated thyroid cancer cell proliferation. Therefore, we concluded that Circ_0067934 attenuated ferroptosis of thyroid cancer cells by miR-545-3p/SLC7A11 signaling. Circ_0067934 may serve as a potential therapeutic target by regulating ferroptosis for the treatment of thyroid cancer.

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Section: Resultsmentioning

confidence: 98%

“…For instance, miR-145 is involved in lncRNA-TUG1-mediated progression of thyroid cancer (11). Furthermore, miR-545-3p has presented tumor suppressor function in various cancers, such as liver cancer, lung cancer, and ovarian cancer (15)(16)(17). But the function of miR-545-3p in thyroid cancer remains obscure.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling

Zhan

2021

Front. Endocrinol.

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“… 18 In contrast, Shi et al proved that miR-545-3p acted as a tumor-suppressive miRNA through targeting HDAC4 to suppress epithelial ovarian cancer proliferation, migration and invasion. 32 These findings suggested that miR-545-3p could exert a tumor-suppressive or tumor-promotive function depending on the type of cancer. More importantly, Chen et al showed that miR-545 was a downregulated miRNA in NB, 20 suggesting that miR-545-3p might act as a tumor suppressor in NB.…”

Section: Discussionmentioning

confidence: 96%

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

Mou¹,

Wang²,

Zhang³

et al. 2021

OTT

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Background: Abnormal expression of long noncoding RNAs (lncRNAs) was often involved in tumorigenesis and radiosensitivity of various cancers. The aim of this study was to explore the biological function and regulatory mechanism of lncRNA long intergenic non-protein coding RNA 1410 (LINC01410) in tumorigenesis and radiosensitivity of neuroblastoma (NB). Methods: The expression of LINC01410, microRNA-329-3p (miR-545-3p) and hexokinase 2 (HK2) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methylthiazolyldiphenyl tetrazolium bromide (MTT) assay, colony formation assay and transwell assay were utilized to detect cell viability, colony formation and cell invasion abilities. Glucose consumption or lactate production was measured by glucose assay kit or lactate assay kit, respectively. The interaction between miR-545-3p and LINC01410 or HK2 was predicted by starBase v2.0 and verified by dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assays. Western blot was used to measure the protein expression of HK2. The mice xenograft model was established to investigate the role of LINC01410 in vivo. Results: LINC01410 and HK2 were highly expressed while miR-545-3p was lowly expressed in NB tissues and cells. LINC01410 knockdown inhibited tumorigenesis by repressing cell proliferation and invasion, and increased the radiosensitivity via inhibiting colony formation rates and glycolysis. LINC01410 knockdown also suppressed tumor growth in vivo. Moreover, miR-545-3p could bind to LINC01410 and its downregulation reversed the effects of LINC01410 knockdown on tumorigenesis and radiosensitivity. Additionally, HK2 was a direct target of miR-545-3p and its overexpression attenuated the effects of miR-545-3p restoration on suppression of tumorigenesis and promotion of radiosensitivity. Besides, LINC01410 functioned as a molecular sponge of miR-545-3p to regulate HK2 expression. Conclusion: LINC01410 interference inhibited tumorigenesis and increased radiosensitivity via regulating miR-545-3p/HK2 axis, providing a novel therapeutic strategy for NB.

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“…For example, up-regulation of miR-545-3p facilitated tumor growth and metastasis by down-regulating metallothionein 1 M in hepatocellular carcinoma [ 27 ]. Conversely, miR-545-3p worked as a tumor inhibitor in epithelial ovarian carcinoma [ 28 ]. Moreover, Zhong et al [ 20 ] uncovered that knockdown of miR-545-3p abrogated the repressive effect of AFAP-AS1 deficiency on the angiogenesis and invasiveness of ECa via controlling VEGFA expression.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA intraflagellar transport 80 facilitates endometrial cancer progression through modulating miR-545-3p/FAM98A signaling

Wang

Guo

Song³

et al. 2022

J Gynecol Oncol

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Objective Endometrial cancer (ECa) is a common gynecological malignancy. Circular RNAs (circRNAs) have been identified as key regulators of human tumorigenesis and development. Herein, we explored the role and mechanism of circular RNA intraflagellar transport 80 (circ-IFT80, also called circ_0067835) in ECa. Methods Circ-IFT80, microRNA-545-3p (miR-545-3p), and family with sequence similarity 98 member A (FAM98A) were quantified by quantitative real-time polymerase chain reaction or Western blot. The biological characteristics of ECa cells were evaluated via Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, transwell, tube formation and flow cytometry assays. Dual-luciferase reporter assay or RNA pull-down assay was employed to verify the binding relationship between miR-545-3p and circ-IFT80 or FAM98A. Xenograft assays were conducted to analyze the effect of circ-IFT80 in vivo. Results Circ-IFT80 and FAM98A were up-regulated, and miR-545-3p was down-regulated in ECa tissues and cells. Knockdown of circ-IFT80 blocked proliferation, migration, invasion and angiogenesis and promoted apoptosis in ECa cells. Moreover, circ-IFT80 harbored a binding site for miR-545-3p, and the effects of circ-IFT80 were mediated by miR-545-3p. FAM98A was a direct target of miR-545-3p, and miR-545-3p hindered ECa cell progression via targeting FAM98A. Circ-IFT80 induced FAM98A expression through miR-545-3p. Furthermore, silence of circ-IFT80 suppressed tumor growth in vivo. Conclusion Circ-IFT80 may promote the malignant progression of ECa cells at least in part by modulating miR-545-3p/FAM98A axis, providing a potential therapeutic target for ECa.

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RETRACTED ARTICLE: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Cited by 16 publications

References 49 publications

Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling

Circular RNA Circ_0067934 Attenuates Ferroptosis of Thyroid Cancer Cells by miR-545-3p/SLC7A11 Signaling

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

Circular RNA intraflagellar transport 80 facilitates endometrial cancer progression through modulating miR-545-3p/FAM98A signaling

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