RETRACTED ARTICLE: LncRNA BCAR4 promotes migration, invasion, and chemo-resistance by inhibiting miR-644a in breast cancer

Abstract

Background Metastasis and drug resistance of breast cancer have become a barrier to treating patients successfully. Long noncoding RNAs (lncRNAs) are known as vital players in cancer development and progression. Methods The RT-qPCR were used to detect the gene expression. Colony formation assay, would healing assay, and transwell assay were performed to investigate oncogenic functions of cells. CCK8 assay was used to detect the cell viability. Western blot was applied to detect the protein level. Dual-luciferase reporter assay was used to determine the relationship between molecules. Mouse orthotopic xenograft tumor models were established to evaluate the effects of BCAR4 on tumor growth and metastasis in vivo. Results LncRNA BCAR4 was significantly increased in breast cancer patients’ tissues and plasma and upregulated in breast cancer cell lines. BCAR4 upregulation was correlated with the TNM stages and decreased after surgical removal of breast tumors. Silencing of BCAR4 suppressed breast cancer cell colony formation, migration, invasion, and xenograft tumor growth and promoted chemo-sensitivity. Mechanistically, BCAR4 facilitates breast cancer migration and invasion via the miR-644a-CCR7 axis of the MAPK pathway. BCAR4 promotes ABCB1 expression indirectly by binding to and down-regulating miR-644a to induce chemo-resistance in breast cancer. Conclusions Our findings provide insights into the oncogenic role of BCAR4 and implicate BCAR4 as a potential diagnostic biomarker and a promising therapeutic agent to suppress metastasis and inhibit chemo-resistance of breast cancer.

lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis

“…For example, continual overexpression of HOTAIR can affect the degree of chromatin openness of tumor cells, thereby regulating gene expression and ultimately promoting the metastasis of breast tumors to the lung in mice [28] . lncRNA BCAR4 accelerates cell migration and infiltration through the miR-644a/CCR7 axis and the MAPK pathway and upregulates ABCB1 to induce chemoresistance in BC [29] . The mechanism of action of lncRNAs is primarily based on their different cellular localizations.…”

Plasma-derived exosomal long noncoding RNAs of pancreatic cancer patients as novel blood-based biomarkers of disease

“…LncRNAs are abnormally expressed in various types of cancers, and differentially expressed lncRNAs in plasma or serum have become an emerging focus area in noninvasive diagnostic applications [23], including intrahepatic cholangiocarcinoma [27], gallbladder carcinoma [28], glioblastoma [29], and prostate carcinoma [30]. Although the functions and molecular mechanisms of most lncRNAs are still unknown, increasing evidence has shown that lncRNAs may play important roles in the occurrence, progression, and metastasis of tumors [31,32]. Recently, an increasing number of lncRNAs have been found to be related to the prognosis of PaCa.…”

Effect of ABC Theory Model on Negative Emotion of Young Patients with Breast Cancer During Treatment

“…Breast cancer is one of the most common malignant tumors that threaten women's health, and with the socioeconomic development and increasing life pressure, breast cancer patients tend to be younger. [1][2][3] Breast cancer in young women is characterized by late stage, high histological grade, high invasiveness, lymphatic vessel emboli, and bone marrow metastasis, compared with Western countries, its mortality and morbidity are increasing year by year. [4][5][6] Young women with breast cancer face more psychological problems, such as anxiety about a higher risk of recurrence, stress about marriage, childbirth, and work, negative emotions such as anxiety and depression often occur in different degrees during treatment, which lead to the decline of quality of life, immune function, poor treatment compliance, and aggravate the adverse reactions of treatment, etc., therefore, it is of great significance to pay attention to the research of negative emotion psychological intervention in young patients with breast cancer.…”