RETRACTED ARTICLE: Litchi Seed Aqueous Extracts play a role in suppression of epithelial-mesenchymal transition, invasion and migration in breast cancer cells

Abstract

We carried out this study to unravel the function of Litchi Seed Aqueous Extracts (LSAE) on biological functions of breast cancer (BC) cells. MTT assay was adopted to measure proliferation of BC cells (MCF7, BT474 and MDA-MB-231) and normal mammary cells (MCF10A) under different time points (24, 48 and 72 h) and different concentrations (50, 100, 200 and 400 μg/mL). MCF-7 cells were selected for subsequent experiments and were grouped into blank group, negative control (NC) group, low-, medium-and high-dose LSAE (L-LSAE, M-LSAE, H-LSAE) groups. Cell viability, invasion, migration and apoptosis were measured by functional assays. Low dosage of LSAE (50 and 100 μg/mL) enhanced proliferation of MCF10A cells, while high dosage of LSAE (200 and 400 μg/mL) suppressed proliferation of MCF10A cells. The proliferation inhibition rate in BT474 and MDA-MB-231cells was increased relative to that in MCF7 cells. MCF-7 cells in the L-LSAE, M-LSAE and H-LSAE groups were rounded and epithelial-like, in which cell survival rate, epithelial-mesenchymal transition (EMT), invasion and migration abilities were reduced versus the blank and NC groups. The tendency in the H-LSAE group was substantially obvious than those in the L-LSAE and M-LSAE groups (both P < 0.05). We found that LSAE is able to inhibit EMT, invasion and migration in BC cells based on concentration and time.

Mechanisms of single herbs and herbal pairs in the treatment of mammary gland hyperplasia: An integrated review

“…Elevated ER and PR levels enhance mammary epithelial cell sensitivity to estrogen, which can trigger mammary gland hyperplasia. When the ER signaling pathway becomes dysregulated, intracellular protein function may be altered, leading to an imbalance in gene expression and resulting in mammary gland cell hyperproliferation or apoptosis arrest [ 41 , 42 ].…”

Astragalus polysaccharide inhibits breast cancer cell migration and invasion by regulating epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway

“…Therefore, the present study (46,47). a number of studies have used the two aforementioned cell lines to study the migration and invasion of breast cancer cells; therefore, the two cell lines were selected for the present study (48)(49)(50). Yang et al (51) demonstrated that aPS inhibited the proliferation of hepatocellular carcinoma cells partly via immunomodulation.…”

Protective effect and efficacy evaluation of lychee seed fermentation broth on oxidative damage of cells

“…Lychee seedt is rich in starch and nutrients, which can be used as medicinal materials. As a traditional Chinese medicinal material, Lychee seed is warm, sweet and slightly bitter, it has the effects of qi clearing, cold removing and pain relieving, etc [3][4] . Lychee seed exhibits an pivotal role in the development of health care products and pharmaceutical research, however its research in the field of cosmetics is not extensive, so this article mainly introduces the role of lychee seed fermentation liquid in anti-inflammatory, antioxidant and safety.…”