Retracted Article: Knockdown of long non-coding RNA OIP5-AS1 suppresses cell proliferation and migration in ox-LDL-induced human vascular smooth muscle cells (hVMSCs) through targeting miR-152-3p/PAPPA axis

Yang, Xiangya; Li, Zhongrui; Zhang, Lei; Wu, Xiaoshan; Kang, Qixin; Li, Li

doi:10.1039/c9ra06614d

Cited by 3 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OIP5‐AS1 promotes apoptosis and inhibits proliferation in HUVECs treated with oxidative low‐density lipoprotein (ox‐LDL) 35 . Yang et al 36 similarly reported that OIP5‐AS1 promotes atherosclerosis in human vascular smooth muscle cells treated with ox‐LDL. Results from these two previous studies 35,36 implicate OIP5‐AS1 in atherosclerosis development.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

lncRNA Oip5‐as1 attenuates myocardial ischaemia/reperfusion injury by sponging miR‐29a to activate the SIRT1/AMPK/PGC1α pathway

Niu

Ling

et al. 2020

Cell Proliferation

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

“…Yang et al 36 similarly reported that OIP5‐AS1 promotes atherosclerosis in human vascular smooth muscle cells treated with ox‐LDL. Results from these two previous studies 35,36 implicate OIP5‐AS1 in atherosclerosis development. However, little was known about the role of OIP5‐AS1 in myocardial diseases.…”

Section: Discussionmentioning

confidence: 97%

lncRNA Oip5‐as1 attenuates myocardial ischaemia/reperfusion injury by sponging miR‐29a to activate the SIRT1/AMPK/PGC1α pathway

Niu

Ling

et al. 2020

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…Zhang et al reported that miR-141 inhibited VSMCs proliferation by targeting PAPPA, implying PAPPA might play a momentous part in the pathogenesis of atherosclerosis [ 32 ]. In addition, Yang et al confirmed that PAPPA was upregulated in human vascular smooth muscle cells under ox-LDL administration [ 33 ]. In this study, PAPPA was elevated in HUVECs by treatment with ox-LDL.…”

Section: Discussionmentioning

confidence: 99%

LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

Gao

Guo

2021

Open Medicine

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) have been verified as vital regulators in human disease, including atherosclerosis. However, the precise role of X-inactive-specific transcript (XIST) in atherosclerosis remains unclear. The proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) exposed to low-density lipoprotein (ox-LDL) were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide, and flow cytometry assays, correspondingly. The western blot assay was used to quantify protein expression. Lactate dehydrogenase activity and the concentrations of inflammatory factors were measured by matched kits. The real-time quantitative polymerase chain reaction (qPCR) was used to determine α-smooth muscle actin, smooth muscle protein 22-α, XIST, miR-98-5p, and pregnancy-associated plasma protein A (PAPPA) levels in HUVECs. The relationship among XIST, miR-98-5p, and PAPPA was analyzed by dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays. We found ox-LDL repressed proliferation and induced inflammation and apoptosis in HUVECs. Loss-of-functional experiment suggested that the downregulation of XIST overturned the ox-LDL-induced effects on HUVECs. Additionally, overexpression of miR-98-5p-induced effects on ox-LDL-stimulated HUVECs was abolished by upregulation of XIST. However, silencing of miR-98-5p strengthened the ox-LDL-induced effects on HUVECs by increasing expression of PAPPA. Mechanistically, XIST could regulate PAPPA expression in ox-LDL-induced HUVECs by sponging miR-98-5p, providing understanding for atherosclerosis.

show abstract

“…For instance, studies have reported that, OIP5-AS1 is a ceRNA in Hepatoblastoma cells through modulating miR-186a-5p/ZEB1 [35] . For another example, OIP5-AS1 as ceRNA positively regulates PAPPA expression through the spongization of miRNA-152-3p [31] . Our research team reported that LncRNA OIP5-AS1 acted as a ceRNA to drive proliferation, invasion, migration and apoptosis of ovarian cancer cells via the spongization of miR-153-3p/KLF5 axis.…”

Section: Discussionmentioning

confidence: 99%

LncRNA OIP5-AS1 Affects the Malignant Biological Behavior of Ovarian Cancer via the miR-153-3p/KLF5 Axis

Wang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Objectives: The purpose of this study was to investigate the expression and clinical significance of LncRNA OIP5-AS1 in ovarian cancer , as well as its effect on malignant biological behavior of ovarian cancer cells. Methods: The expression of OIP5-AS1, miR-153-3p and KLF5 in ovarian cancer (OC) tissues and cells were detected by RT-qPCR. Western Blotting was used to detect KLF5 expression. The expression patterns of OIP5-AS1, U6 and GAPDH in nuclear and cytoplasm fractions were detected using qRT-PCR. Besides, CCK-8 assay, clone formation assay, transwell, scratch test, and flow cytometry were respectively used to detect the cell activity, proliferation, invasiveness, healing of cells, and apoptosis rate of OC cells. Furthermore, The interaction between OIP5-AS1 and miR-153-3p and between miR-153-3p and KLF5 were verified by luciferase reporter assay, and the correlations among these three genes were analyzed.Results: OIP5-AS1 expression was up-regulated in ovarian cancer cell lines and tissues. Si-OIP5-AS1 inhibited cell proliferation, invasion and migration, and induced the apoptosis to a certain extent. Subcellular fraction assay revealed the location of OIP5-AS1 was mainly situated in the cytoplasm. In addition, miR-153-3p was a target of OIP5-AS1, and KLF5 was directly targeted by miR-153-3p. Si-OIP5-AS1 inhibited KLF5 expression, miR-153-3p inhibitor promoted KLF5 expression, and si-KLF5 inhibited OIP5-AS1 expression. Interestingly, expression of OIP5-AS1 and miR-153-3p, and expression of miR-153-3p and KLF5 were negatively correlated, while expression of OIP5-AS1 and KLF5 was positively correlated. In addition, si-KLF5 inhibited the malignant biological behavior of ovarian cancer cells, while miR-153-3p inhibitor had the opposite effect. Most importantly, the addition of si-OIP5-AS1 to mir-153-3p silenced cells could reverse the promotion effect of miR-153-3p inhibitor on the malignant biological behavior of ovarian cancer cells.Conclusions: OIP5-AS1 can be used as an effective prognostic indicator of ovarian cancer, which has the potential to be a new drug target.

show abstract

Retracted Article: Knockdown of long non-coding RNA OIP5-AS1 suppresses cell proliferation and migration in ox-LDL-induced human vascular smooth muscle cells (hVMSCs) through targeting miR-152-3p/PAPPA axis

Abstract: Knockdown of OIP5-AS1 suppressed ox-LDL-treated hVMSCs proliferation and migration; overexpression of miR-152 played the similar role of OIP5-AS1 knockdown; OIP5-AS1 functioned as ceRNA to regulate PAPPA expression through sponging miR-152.

Cited by 3 publications

References 34 publications

lncRNA Oip5‐as1 attenuates myocardial ischaemia/reperfusion injury by sponging miR‐29a to activate the SIRT1/AMPK/PGC1α pathway

lncRNA Oip5‐as1 attenuates myocardial ischaemia/reperfusion injury by sponging miR‐29a to activate the SIRT1/AMPK/PGC1α pathway

LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs

LncRNA OIP5-AS1 Affects the Malignant Biological Behavior of Ovarian Cancer via the miR-153-3p/KLF5 Axis

Contact Info

Product

Resources

About