RETRACTED ARTICLE: High bioavailablilty iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro (Caco-2 model) and in vivo (Gallus gallus)

Tako, Elad; Hoekenga, Owen A.; Kochian, Leon V.; Glahn, Raymond P.

doi:10.1186/1475-2891-12-3

Cited by 50 publications

(56 citation statements)

References 48 publications

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“…3 Values are mean ± SEM (cumulative weekly from day 0). 4,5 Fe bioavailability was calculated as hemoglobin maintenance efficiency ( HME ) [5-9,15]:

HME = \frac{Hb Fe, mg (italicfinal) - Hb Fe, mg (italicinitial)}{Total Fe Intake, mg} \times 100 .

Where Hb-Fe = total body hemoglobin Fe. Hb Fe was calculated from hemoglobin concentrations and estimates of blood volume based on body weight (a blood volume of 85 mL per kg body weight is assumed) [5-9,15,22]: Hb - Fe(mg) = B. W. (kg) × 0.085 L blood/kg × Hb (g/L) × 3.35 mg Fe/g Hb.…”

Section: Resultsmentioning

confidence: 99%

Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgarisL.)

Tako

Beebe

Reed

et al. 2014

Nutr J

Self Cite

153

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BackgroundOur objective was to determine if a biofortified variety of black bean can provide more bioavailable-iron (Fe) than a standard variety. Two lines of black beans (Phaseolus-vulgaris L.), a standard (DOR500; 59μg Fe/g) and biofortified (MIB465; 88μg Fe/g) were used. The DOR500 is a common commercial variety, and the MIB465 is a line developed for higher-Fe content. Given the high prevalence of Fe-deficiency anemia worldwide, it is important to determine if Fe-biofortified black beans can provide more absorbable-Fe.MethodsBlack bean based diets were formulated to meet the nutrient requirements for the broiler (Gallus-gallus) except for Fe (dietary Fe-concentrations were 39.4±0.2 and 52.9±0.9 mg/kg diet, standard vs. biofortified, respectively). Birds (n=14) were fed the diets for 6-weeks. Hemoglobin-(Hb), liver-ferritin and Fe-related transporter/enzyme gene-expression were measured. Hemoglobin-maintenance-efficiency and total-body-Hb-Fe values were used to estimate Fe-bioavailability.ResultsHemoglobin-maintenance-efficiency values were higher (P<0.05) in the group consuming the standard-Fe beans on days 14, 21 and 28; indicating a compensatory response to lower dietary-Fe. Final total-Hb-Fe body content was higher in the biofortified vs. the standard group (26.6±0.9 and 24.4±0.8 mg, respectively; P<0.05). There were no differences in liver-ferritin or in expression of DMT-1, Dcyt-B, and ferroportin. In-vitro Fe-bioavailability assessment indicated very low Fe-bioavailability from both diets and between the two bean varieties (P>0.05). Such extremely-low in-vitro Fe-bioavailability measurement is indicative of the presence of high levels of polyphenolic-compounds that may inhibit Fe-absorption. High levels of these compounds would be expected in the black bean seed-coats.ConclusionsThe parameters of Fe-status measured in this study indicate that only a minor increase in absorbable-Fe was achieved with the higher-Fe beans. The results also raise the possibility that breeding for increased Fe-concentration elevated the levels of polyphenolic-compounds that can reduce bean Fe-bioavailability, although the higher levels of polyphenolics in the higher-Fe beans may simply be coincidental or an environmental effect. Regardless, Fe-biofortified beans remain a promising vehicle for increasing intakes of bioavailable-Fe in human populations that consume high levels of these beans as a dietary staple, and the bean polyphenol profile must be further evaluated and modified if possible in order to improve the nutritional quality of higher-Fe beans.

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“…3 Values are mean ± SEM (cumulative weekly from day 0). 4,5 Fe bioavailability was calculated as hemoglobin maintenance efficiency ( HME ) [5-9,15]:

HME = \frac{Hb Fe, mg (italicfinal) - Hb Fe, mg (italicinitial)}{Total Fe Intake, mg} \times 100 .

Section: Resultsmentioning

confidence: 99%

Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgarisL.)

Tako

Beebe

Reed

et al. 2014

Nutr J

Self Cite

153

View full text Add to dashboard Cite

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“…However, a genetic approach may not always be adequate due to several interacting factors, including lack of readily available germplasm, unfavorable genetic, physiological, and chemical interactions within the ionome [38], and negative relationships with grain yield [17,20]. These perceived shortcomings of genetic biofortification recently led to promoting molecular breeding [38,39] as a means of improving [nutrient] and bioavailability of micronutrients in maize and other grain crops.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Maize Kernels from a Breeding Program for Protein Quality III: Ionome Profiling

Jaradat

Goldstein

2018

Agronomy

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Densities of single and multiple macro-and micronutrients were estimated in the mature kernels of 1348 accessions in 13 maize genotypes. The germplasm belonged to stiff stalk (SS) and non-stiff stalk (NS) heterotic groups (HGs) with one (S1) to four (S4) years of inbreeding (IB), or open pollination (OP), and with opaque or translucent endosperm (OE and TE, respectively). Indices were calculated for macronutrients (M-Index), micronutrients (m-Index) and an index based on Fe and Zn densities (FeZn-Index). The objectives were to (1) build predictive models and quantify multivariate relationships between single and multiple nutrients with physical and biochemical constituents of the maize kernel; (2) quantify the effects of IB stages and endosperm textures, in relation to carbon and nitrogen allocation, on nutrients and their indices; and (3) develop and test the utility of hierarchical multi-way classification of nutrients with kernel color space coordinates. Differences among genotypes and among IB stages accounted for the largest amount of variation in most nutrients and in all indices, while genotypic response to IB within HGs explained 52.4, 55.9, and 76.0% of variation in the M-Index, m-Index, and FeZn-Index, respectively. Differences in C and N allocation among HGs explained more variation in all indices than respective differences in allocation among endosperm (E) textures, while variation decreased with sequential inbreeding compared to OP germplasm. Specific color space coordinates indicated either large macronutrient densities and M-Index, or large micronutrient densities, m-Index, and FeZn-Index. These results demonstrated the importance of genotypes and the C:N ratio in nutrient allocation, as well as bivariate and multiple interrelationships.

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“…In spite of its high heritability, only three modest QTL for FeGC were detected, which confirms that this trait is under the control of many small QTL (Šimić et al 2012), whereas there were ten QTL for FeGB accounting for 54 % of the variance observed in samples from a single year/location trial. In vitro digestion/Caco 2 cell culture and in vivo poultry models were used to compare maize lines with contrasting Fe bioavailability (Tako et al 2013). A favorable matrix for absorption could explain the Fe-bioavailability levels among these lines.…”

Section: Genomics-led Improvement For Enhancing Genetic Gainsmentioning

confidence: 99%

Breeding Open-Pollinated, Hybrid and Transgenic Outcrossing Species

Ríos

2015

Plant Breeding in the Omics Era

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Outcrossing is the transfer of pollen grains from the anther of a flower to the stigma of another flower in a different plant of the same species in the angiosperms by the action of wind, insects, or other vectors, thereby enabling fertilization and reproduction. It allows for species diversity because it combines genetic information from different plants, and may lead to heterozygosity when involving two genetically distinct plants. About 50 % of crops show outcrossing and their reproductive systems include devices promoting it, for example, unisexuality, maturing time, self-incompatibility, and male sterility. Various outcrossing species may have a small amount (5-10 %) of selfing. Outcrossing species exhibit mild to severe inbreeding depression and significant heterosis. Maize, onion, and rye are among outcrossing species, while cotton shows up to 30 % of outcrossing. Composite, hybrid, and synthetic cultivars are the aim for breeding outcrossing species, when their seed production is feasible and profitable. A composite cultivar ensues from mixing seeds of outstanding lines (often up to 20) and encouraging open pollination among the mixed lines, while a synthetic cultivar results from crossing (6-8) lines that combine well with each other and are kept by open pollination in isolation. Pedigree, doubled haploids, and backcrossing are used for inbred line development, while recurrent selection methods accumulate favorable alleles in a breeding population. Plant genetic engineering and DNA marker-aided breeding are among the biotechnology tools for improving maize, cotton, cassava, and other outcrossing species. MaizeMaize (Zea mays) is the cereal with the largest global output and cash value. It is among the most important staple food crops, a source of feeding grains and silage for livestock, and used by the industry for ethanol, oil, and starch. Its average global per capita consumption is below that of wheat, rice, and potato because of the high

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RETRACTED ARTICLE: High bioavailablilty iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro (Caco-2 model) and in vivo (Gallus gallus)

Cited by 50 publications

References 48 publications

Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgarisL.)

Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgarisL.)

Diversity of Maize Kernels from a Breeding Program for Protein Quality III: Ionome Profiling

Breeding Open-Pollinated, Hybrid and Transgenic Outcrossing Species

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