RETRACTED ARTICLE: Circular RNA circ_0000517 regulates hepatocellular carcinoma development via miR-326/IGF1R axis

Abstract

Background: Circular RNAs (circRNAs) play vital roles in hepatocellular carcinoma development. However, the role and mechanism of circRNA hsa_circ_0000517 (circ_0000517) in hepatocellular carcinoma development were largely unknown. Methods: 45 paired tumor and adjacent nontumor samples were collected from hepatocellular carcinoma patients. The levels of circ_0000517, miR-326 and insulin-like growth factor type 1 receptor (IGF1R) were detected via quantitative reverse transcription polymerase chain reaction or western blot. Cell viability, colony ability, migration, invasion and glycolysis were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, western blot, transwell assay, glucose consumption, lactate production or adenosine triphosphate (ATP) production. The target correlation between miR-326 and circ_0000517 or IGF1R was analyzed via dual-luciferase reporter analysis. The function of circ_0000517 in vivo was assessed via xenograft model. Results: circ_0000517 expression was elevated in hepatocellular carcinoma tissues and cell lines. circ_0000517 knockdown suppressed cell viability, colony formation, migration, invasion and glycolysis. miR-326 was sponged via circ_0000517 and miR-326 knockdown reversed the effect of circ_0000517 silence on hepatocellular carcinoma development. miR-326 overexpression inhibited hepatocellular carcinoma development through targeting IGF1R. circ_0000517 knockdown decreased IGF1R expression by modulating miR-326. circ_0000517 downregulation reduced xenograft tumor growth. Conclusion: circ_0000517 knockdown repressed hepatocellular carcinoma development in vitro and in vivo by modulating miR-326 and IGF1R.

lncRNA PCAT6 facilitates cell proliferation and invasion via regulating the miR‑326/hnRNPA2B1 axis in liver cancer

“…Evidence has suggested that microRNA (miRNA/miR) expression is abnormal in different types of tumor tissues and it is well-known that miRNAs play notable roles in tumor development and cancer therapy (19). To date, low expression levels of miR-326 have been observed in HCC tissues and cell lines, and miR-326 has been found to exert a regulatory effect on the proliferation, migration and invasion of HCC cells (20). Nevertheless, to the best of the authors' knowledge, there is currently no evidence regarding the interaction between PCAT6 and miR-326 in liver cancer.…”

Dysregulated circ_0004913, circ_0008160, circ_0000517, and their potential as biomarkers for disease monitoring and prognosis in hepatocellular carcinoma

“…12 Besides, circ_0000517 knockdown decreases HCC cell viability, colony formation, cell migration, cell invasion, and glycolysis by mediating the miR-326/ insulin-like growth factor 1 receptor (IGF1R) axis. 13 Although existing evidence is quite insufficient, these experimental studies of circ_0004913 and circ_0000517 may explain their potential clinical values found in our study to some extent.…”

“…[9][10][11] Previously, three circRNAs (circ_0004913, circ_0008160, and circ_0000517) are reported to be dysregulated in HCC tissues (GSE94508 and GSE97332), and these three circRNAs are also confirmed by qPCR to be dysregulated in both HCC tissues and HCC cells; more interestingly, two of them are revealed to be potential regulators of HCC pathogenesis as well. [12][13][14] Nevertheless, the possible clinical values of these three circRNAs remain to be elusive in HCC.…”

Circular RNAs in Hepatocellular Carcinoma: Emerging Functions to Clinical Significances

“…Mechanistically, circ-PRMT5 promotes HCC proliferation, migration and glycolysis by miR-188-5p sponging to regulate HK2 (104). Furthermore, circ-0000517 promotes HCC progression and glycolysis via miR-326/IGF1R (105). In total, circRNAs are novel ncRNAs, and only a few studies have reported on the altered metabolic reprogramming regulated by circRNAs in HCC.…”