Retinoprotective Effect of 2-Ethyl-3-hydroxy-6-methylpyridine Nicotinate

Peresypkina, Anna A.; Pazhinsky, Anton; Даниленко, Л. М.; Lugovskoy, Sergey S.; Покровский, М. В.; Бесхмельницына, Е. А.; Solovev, Nikolai; Pobeda, Anna; Korokin, Mikhail; Levkova, E. A.; Gubareva, V.O.; Корокина, Л. В.; Martynova, O. M.; Солдатов, Владислав О.; Покровский, В. М.

doi:10.3390/biology9030045

Cited by 8 publications

(8 citation statements)

References 41 publications

(53 reference statements)

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“…It is widely accepted that I/R injury is caused by the increased generation of reactive oxygen species (ROS) during the process of I/R, including superoxide (O 2 ), hydrogen peroxide (H2O2) and hydroxyl radicals (OH -). The overproduction of ROS can induce apoptosis in the retina [3,4,5]. As a result, inflammation and cell apoptosis are considered to be major causes of the pathological changes following I/R injury.…”

Section: оригинальная статья Original Articlementioning

confidence: 99%

Studies to elucidate the effect and antiapoptotic mechanism of 2-ethyl-3-hydroxy-6- methylpyridine-n-acetyltaurinate in a rat model of retinal ischemia–reperfusion

2022

RR. in B.

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Background: The study of possible ways of effective correction of retinal ischemia-reperfusion injury, which accompanies a number of eye diseases, is relevant today. The aim of the study: To study the retinoprotective effect and antiapoptotic mechanism of 2-Ethyl-3-hydroxy-6-methyl-pyridine-N-acetyltaurinate (EHMP-NAT) in a rat model of retinal ischemia–reperfusion (I/R). Materials and methods: A pathology model with an increase in intraocular pressure (IOP) to 110 mmHg was used. The retinoprotective effect of EHMP-NAT at a dose of 4.4 mg/kg/day, in comparison with emoxipine and taurine in equimolar doses, was estimated by the changes in the retinal microcirculation, electroretinograms (the b/a coefficient), and retinal caspase-3, NF-κB p65, p53 gene expressions in Wistar rats. Results: The use of EHMP-NAT led to an increase in the retinal microcirculation level to 756.5 (median) perfusion units in comparison with emoxipine (p = 0.045) and taurine (p = 0.00029). The b/a coefficient increased in comparison with the group with emoxipine (p = 0.0099) and with the group with taurine (p = 0.015). In the group with EHMP-NAT, the caspase-3 gene expression decreased reliably in comparison with emoxipine (p = 0.0002) and with taurine (p = 0.0028); the NF-κB p65 gene expression decreased in comparison with emoxipine (p = 0.0009) and with taurine (p = 0.0022); the p 53 gene expression decreased in comparison with emoxipine (p = 0.0022) and with taurine (p = 0.0009). Conclusion: Based on the data obtained, in correction of retinal I/R by EHMP-NAT, improvements in the retinal microcirculation, functional state, and caspase-3, NF-κB p65, p53 gene expressions were more pronounced than in monotherapy with emoxipine or taurine.

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Section: оригинальная статья Original Articlementioning

confidence: 99%

Studies to elucidate the effect and antiapoptotic mechanism of 2-ethyl-3-hydroxy-6- methylpyridine-n-acetyltaurinate in a rat model of retinal ischemia–reperfusion

2022

RR. in B.

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“…The administration of vitamin PP (nicotinic acid) has long been known to promote beneficial effects on blood lipid and cholesterol profiles [31]. Besides, vitamin PP is a "scavenger" of the superoxide radical from which hydroxyl is subsequently formed, which has a powerful destructive potential against cell structures and enzymes [32].…”

Section: Microscopy Of Kidneymentioning

confidence: 99%

Correction of metabolic disturbances by functional food compositions in experimental obesity in CD-1 and agouti-yellow mice

Lebedev¹,

Peresypkina

Gustinovich³

et al. 2022

FFHD

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Background: To determine the most promising composition, the possibility of correction of metabolic disturbances was investigated with the use of functional food compositions in CD1 and agouti-yellow mice in experimental obesity.Methods: Agouti-yellow and CD1 mice were divided into 10 groups (control, 1/1, 1/9, 2/1, 2/9 in each line) according to diets. For 4 weeks, the groups ate a high-calorie diet. Starting with week 5th, composition 1 was added to the diet in groups 1/1 (60% lard + 39% standard laboratory feed + 1% composition 1 + 10% fructose solution), 1/9 (60% lard + 31% standard laboratory feed + 9% composition 1 + 10% fructose solution); composition 2 was added to the diet in groups 2/1 (60% lard + 39% standard laboratory feed + 1% composition 2 + 10% fructose solution), 2/9 (60% lard + 39% standard laboratory feed + 9% composition 2 + 10% fructose solution). On the day 56th, each animal was weighed, blood was taken for biochemical analysis (glucose, LDL, cholesterol and HDL) and organs (the skin of the anterior abdominal wall, skeletal muscle tissue in the area of the lateral surface of the thigh, liver, kidneys with perirenal tissue, pancreas, and thymus) were taken for histological examination.Results: In agouti-yellow mice, group 2/9, the greatest decrease in blood glucose level was observed compared to the control group by 34.6% (p < 0.05). Consumption of compositions in groups 1/9 and 2/9 led to a significant decrease in LDL and a significant decrease in total cholesterol level by 21.9% (p < 0.05) and 18.8% (p < 0.05) respectively compared to the control group. HDL was significantly higher in groups 1/9, 2/9 than in the control group. In CD-1 mice, group 2/9 showed the best result in glucose level (mean 5.78 mmol/l) among all groups receiving functional food compositions and the control group. The most pronounced decrease in LDL was observed in groups 1/9, 2/9 compared to the control group (p < 0.05). HDL value in group 2/9 significantly exceeded the mean value in the control group, by 54.8% (p < 0.05). Body mass of agouti-yellow mice and CD-1 mice in groups 1/1, 2/1, 1/9, and 2/9 significantly decreased compared to control groups. According to the results of histological examination of organs and tissues, functional food composition 2 turned out to be the most promising.Conclusion: According to the results of the study, the most promising composition was selected (composition 2). Feeding animals with this composition improved histological pictures of studied organs and tissues, biochemical blood parameters and effectively reduced body mass in both experimental animal lines, CD-1, and agouti-yellow mice, compared to the control groups. Keywords: CD-1 mice, agouti-yellow mice, experimental obesity, LDL, cholesterol

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“…The retinopathy model was created by transiently increasing the IOP to 110 mmHg (Peresypkina et al 2020) by applying mechanical pressure on the anterior chamber of the eye for 30 minutes. Next, a 72-hour reperfusion followed and after that the drugs were administered.…”

Section: Design Of the Experimentsmentioning

confidence: 99%

“…For between-group comparisons, the Student's t-test for independent groups was used if the distribution of variables was normal; otherwise, the U-test (Mann-Whitney test) was performed. P-values ≤ 0.05 were considered statistically significant (Peresypkina et al 2020).…”

Section: Statistical Data Managementmentioning

confidence: 99%

Study to elucidate the pharmacological activity of retinalamin in a rat model of ischemic retinopathy

Pobeda¹,

Kalatanova²,

Abasheva³

et al. 2021

RRP

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Introduction: Over the past few years, the incidence of retinal ischemic disorders has been increasing, due to a rising prevalence of such socially burdensome diseases as diabetes and hypertension, which ultimately lead to ocular vascular pathology. The identification of new treatment options that would prevent retinal neuron death is a crucial task of modern pharmacology. Materials and methods: The research was carried out on male Wistar rats. Retinopathy was modeled by inducing a 30-min ischemic episode, with a 72-hour period of reperfusion and subsequent administration of Retinalamin and Emoxypine for 10 days. The effectiveness of the drugs was evaluated by electroretinographic, ophthalmoscopic and morphological assessments. Results and discussion: On Day 14 of the experiment, a dose-dependent preservation of the electroretinogram b-wave/a-wave amplitude ratio was observed in the animals treated with Retinalamin depending on a dose (1.39±0.06, 1.46±0.03 and 1.49±0.04 in low (0.214 mg/kg), medium (0.428 mg/kg) and high (0.857 mg/kg) Retinalamin dose groups, respectively). The ophthalmoscopic picture of the fundus oculi also improved following the treatment with Retinalamin (1.42, 1.69 and 1.90 times lower ophthalmoscopic scores compared to placebo-treated animals in low, medium and high dose groups, respectively). The morphologic “coefficient of change” applied to ganglion cell layer was 2.2, 1.7 and 1.6 points in low, medium and high dose Retinalamin groups, respectively. These results are significantly different from both intact and placebo group (p<0.05). Based on the aforementioned experimental findings, we conclude that Retinalamin has a retinoprotective effect and is superior to the drug of comparison (Emoxypine). Conclusion: The greatest neuroprotective effects were shown in the groups receiving Retinalamin. In these groups, the ERG b-wave/a-wave amplitude ratio was preserved, the ophthalmoscopic picture was less pathologic and retinal morphology features were close to those of the intact retina.

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Retinoprotective Effect of 2-Ethyl-3-hydroxy-6-methylpyridine Nicotinate

Cited by 8 publications

References 41 publications

Studies to elucidate the effect and antiapoptotic mechanism of 2-ethyl-3-hydroxy-6- methylpyridine-n-acetyltaurinate in a rat model of retinal ischemia–reperfusion

Studies to elucidate the effect and antiapoptotic mechanism of 2-ethyl-3-hydroxy-6- methylpyridine-n-acetyltaurinate in a rat model of retinal ischemia–reperfusion

Correction of metabolic disturbances by functional food compositions in experimental obesity in CD-1 and agouti-yellow mice

Study to elucidate the pharmacological activity of retinalamin in a rat model of ischemic retinopathy

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