Retinol Administration to Superovulated Ewes Improves In Vitro Embryonic Viability1

Eberhardt, Dawn M.; Will, W.; Godkin, James D.

doi:10.1095/biolreprod60.6.1483

Cited by 43 publications

(40 citation statements)

References 37 publications

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“…The oocyte's developmental competence is enhanced by retinoid support during oocyte intrafollicular growth in the cow (Shaw et al, 1995), sheep (Eberhardt et al, 1999), and gilt (Whaley et al, 1997;Whaley et al, 2000), as in rabbits that have high levels of vitamin A (Besenfelder et al, 1996). In agreement with these findings, the addition of 9-cis-RA to in vitro maturation (IVM) cultures stimulated cortical granule migration in bovine oocytes both matured and meiotically arrested for a 24-hr culture period (Duque et al, 2002).…”

Section: Introductionmentioning

confidence: 55%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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The presence of retinoic acid (RA) during in vitro maturation (IVM) improves bovine oocyte quality and developmental potential. In this work, we investigated the underlying molecular mechanisms. Cumulus-oocyte complexes were meiotically arrested by roscovitine and/or matured in defined medium containing RA, 1% ethanol (vehicle), or no additives. Cumulus-free oocytes were analyzed for poly-(A) mRNA contents and relative mRNA expression of genes involved in cell cycle regulation (cyclin B1 and H1) and antioxidative defence (Mn-superoxide dismutase and glucose-6-phosphate dehydrogenase). Poly-(A) mRNA increased after meiotic inhibition and decreased with IVM completion, both in meiotically arrested and permissively matured oocytes, i.e., matured without previous meiotic arrest. RA dramatically increased poly-(A) mRNA in meiotically arrested oocytes, but more than half of the poly-(A) mRNA disappeared during maturation. Irrespective of oocyte origin, transcripts were detected for all the genes analyzed. IVM, with or without previous meiotic inhibition, increased expression of cyclin B1 and glucose-6-phosphate dehydrogenase, and decreased cyclin H1 and Mn-superoxide dismutase. Except for a decreasing of Mn-superoxide dismutase in meiotically arrested and matured oocytes, RA did not affect mRNA expression. Ethanol led to an abnormal poly-(A) mRNA profile and expression of all the genes analyzed. RA does not modify expression of cyclin B1 and HI genes in the bovine oocyte, and probably does not generate oxidative stress. In addition, RA enhanced mRNA amount as measured by poly-(A) mRNA contents.

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Section: Introductionmentioning

confidence: 55%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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“…Administration of retinol with super ovulation increased the number of transferable embryos but did not affect ovulation rate (Shaw et al, 1995). Retinol treatment in combination with super ovulation improves the developmental competence of resultant embryos (Eberhardt et al, 1999). Earlier studies have reported the presence of retinol in follicular fluid (Schweigert and Zucker, 1988;Brown et al, 2003), stating that vitamin-A concentrations to be highest in large nonatretic follicles and lowest in small atretic follicles correlated with estradiol concentrations.…”

Section: Introductionmentioning

confidence: 98%

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Thiyagarajan

Valivittan

2009

Animal

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Vitamin A is a well-known antioxidant and is essential for embryonic development, growth and differentiation. Oxidative stress is involved in the etiology of defective embryo development. The present study evaluated whether the presence of all-trans retinol (0, 1, 2, 5 and 10 mM) in maturation medium or embryo culture medium would enhance the developmental competence of preimplantation buffalo embryos in vitro. In experiment I, cumulus oocytes complex were matured with varying concentrations of all-trans retinol. Treatment with 5 mM all-trans retinol improved the blastocyst formation ( P , 0.001) when compared with control and significant increase ( P , 0.01) in total cell number was observed in 5 mM group when compared with control. Supplementation of all-trans retinol in embryo culture medium for the entire culture period under 5% O 2 and 20% O 2 was tested in experiments II and III, respectively. Supplementation of 10 mM all-trans retinol under 5% O 2 , significantly reduced blastocyst formation and cell numbers. Presence of 5 mM all-trans retinol under 20% O 2 enhanced the frequency of blastocyst formation and total cell number ( P , 0.001) when compared with control. DNA damage of individual embryos cultured under 20% oxygen concentration was measured by the comet assay. Supplementation of 5 mM all-trans retinol significantly reduced the comet tail ( P , 0.001) when compared with control. Supplementation of all-trans retinol in embryo culture medium for first 72 h of the 8-day culture period under 5% O 2 was tested in experiment IV. Addition of 5 mM all-trans retinol resulted in significant increase in blastocyst rate and total cell number ( P , 0.001) when compared with control. Our results demonstrate that addition of all-trans retinol to maturation or embryo culture medium may enhance the developmental competence of buffalo embryos in vitro by enhancing blastocyst formation rate and total cell number.

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“…It has been reported that the developmental competence of the oocyte is enhanced by ROH treatment in the cow (Shaw et al 1995), sheep (Eberhardt et al 1999) and gilt (Whaley et al 1997(Whaley et al , 2000, as well as in rabbits that have high blood levels of vitamin A (Besenfelder et al 1996). In studies involving cows (Shaw et al 1995), sheep (Eberhardt et al 1999) and gilts (Whaley et al 1997(Whaley et al , 2000 the effects of retinol on follicular development were monitored and recorded 4 days post-administration.…”

Section: Introductionmentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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Retinol Administration to Superovulated Ewes Improves In Vitro Embryonic Viability1

Cited by 43 publications

References 37 publications

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

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