Retinoid-sensitive cells and cell lines

Identification of a major poly‐N‐acetyllactosamine‐containing cell‐surface glycoprotein of mouse teratocarcinoma cells

“…However, only a few percent of LAMP-1 and none of LAMP-2 is exposed on the cell surface [43]. This was also correlated with the finding that very little LAMP was detectable with the surface labelling method (Fig.…”

“…Although the glycoprotein differed by electrophoretic mobility from previously characterised poly-N-acetyllactosamine-containing glycoproteins of F9 teratocarcinoma cells, fibronectin [42], LAMP-1, LAMP-2 and laminin [43], this did not exclude the possibility that the glycoprotein represented a differentially glycosylated form of one of these glycoproteins. The immunoreactivity of the glycoprotein with antibodies to the known poly-N-acetyllactosamine carriers was therefore investigated.…”

“…Like LAMP-1, LAMP-2 and laminin [43], the glycoprotein studied shows a significant increase during retinoic-acidinduced differentiation that starts after a lag of about 48 h. The increase is similarly accompanied by an apparent increase in the size of the molecules, as indicated by an increase in the slower-moving species of the glycoprotein in gel electrophoresis. This change can been ascribed to an increase in the glycosyltransferases that catalyse the biosynthesis of poly-N-acetyllactosamine chains in these glycoproteins [27].…”

“…Lysosome-associated glycoproteins (LAMP-1 and LAMP-2) have been regarded as the major carriers of poly-N-acetyllactosamine chains in many cells [44], but only a small percentage of these glycoproteins is expressed on the surface of F9 cells [43]. Antibodies to the LAMPS did not bind to the purified glycoprotein on blotting membranes, although they reacted with the corresponding LAMP glycoproteins of the cells (Fig.…”

Soluble Form of Heparin-binding EGF-like Growth Factor Contributes to Retinoic Acid-induced Epidermal Hyperplasia

“…All-trans retinoic acid (tRA) is the major biologically active metabolite of vitamin A. tRA and related synthetic retinoids have been used widely in the therapy of several skin disorders, including acne (Pedace and Stoughton, 1971), photo-aged skin (Kligman et al, 1984), and psoriasis (Goldfarb et al, 1988). However, the effects of retinoids are often conflicting: retinoids can either stimulate or inhibit cell proliferation depending on the cell type affected or on the conditions used in cell cultivation (Amos and Lotan, 1990;Fisher and Voorhees, 1996;Rogers, 1997;Varani et al, 1989Varani et al, , 1991. Although the mechanism by which retinoids regulate the proliferation of epidermal cells is not completely understood, it has been shown that treatment of normal adult human skin and keratinocytes with tRA not only leads to thickening of the skin but also induces significant expression of several genes including HB-EGF (Bernard et al, 2002;Stoll and Elder, 1998;Varani et al, 2001).…”