Retinoic acid signaling and neuronal differentiation

Janesick, Amanda; Wu, Stephanie; Blumberg, Bruce

doi:10.1007/s00018-014-1815-9

Cited by 235 publications

(194 citation statements)

References 257 publications

Supporting

Mentioning

186

Contrasting

Order By: Relevance

“…Because regulating the switch between proliferation and differentiation is fundamental to neurogenesis (Janesick et al . ), this evidence is supportive of the crucial role of ERRγ in neuronal differentiation.…”

Section: Discussionsupporting

confidence: 75%

Estrogen‐related receptor gamma regulates dopaminergic neuronal phenotype by activating GSK3β/NFAT signaling in SH‐SY5Y cells

Lim

Choi

2015

Journal of Neurochemistry

View full text Add to dashboard Cite

The orphan nuclear receptor estrogen-related receptor gamma (ERRc) is highly expressed in the nervous system during embryogenesis and in adult brains, but its physiological role in neuronal development remains unknown. In this study, we evaluated the relevance of ERRc in regulating dopaminergic (DAergic) phenotype and the corresponding signaling pathway. We used retinoic acid (RA) to differentiate human neuroblastoma SH-SY5Y cells. RA induced neurite outgrowth of SH-SY5Y cells with an increase in DAergic neuron-like properties, including up-regulation of tyrosine hydroxylase, dopamine transporter, and vesicular monoamine transporter 2. ERRc, but not ERRa, was up-regulated by RA, and participated in RA effect on SH-SY5Y cells. ERRc over-expression enhanced mature DAergic neuronal phenotype with neurite outgrowth as with RA treatment; and RA-induced increase in DAergic phenotype was attenuated by silencing ERRc expression. ERRc appears to have a crucial role in morphological and functional regulation of cells that is selective for DAergic neurons. Polo-like kinase 2 was up-regulated in ERRc-over-expressing SH-SY5Y cells, which was involved in phosphorylation of glycogen synthase kinase 3b and resulting downstream activation of nuclear factor of activated T cells. The likely involvement of ERRc in regulating the DAergic neuronal phenotype makes this orphan nuclear receptor a novel target for understanding DAergic neuronal differentiation.

show abstract

Section: Discussionsupporting

confidence: 75%

Estrogen‐related receptor gamma regulates dopaminergic neuronal phenotype by activating GSK3β/NFAT signaling in SH‐SY5Y cells

Lim

Choi

2015

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…The decreased expression of astrocytic protein markers coinciding with increased expression of neuron-specific protein markers suggests that RA treatment biases 3D hNPC differentiation outcomes toward neuronal fates, which is consistent with previous literature reports in 2D culture (Takahashi et al, 1999). Meanwhile, the decreased NES expression could signify a more rapid departure from the progenitor stage and is in agreement with the potent morphogenic activity of RA, which is an endogenous agonist of cellular retinoic acid receptor proteins that induces differentiation of many tissues, including terminal neural differentiation (Janesick et al, 2015). …”

Section: Resultsmentioning

confidence: 59%

“…Indeed, every combination containing RA resulted in significantly higher TUBB3 expression compared to spontaneous differentiation. Identification of RA as a strong inducer of neuronal differentiation was not surprising, as RA is well known to induce neuronal differentiation on multiple types of neural progenitor cells (Erceg et al, 2008; Janesick, Wu, & Blumberg, 2015; Takahashi, Palmer, & Gage, 1999). It was interesting, however, that in our screen RA treatment alone was not identified as being statistically significant, but combined treatment of RA with other led to more pronounced effects, which is consistent with its differentiation inducing effects reported in the literature (Encinas et al, 2000; Takahashi et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

High‐throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture

Nierode

Gopal

Kwon

et al. 2018

Biotech & Bioengineering

View full text Add to dashboard Cite

Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell culture models have been used increasingly for in vitro cell culture because they may better model endogenous tissue environments. However, detailed studies of stem cell differentiation within 3D cultures remain limited, particularly with respect to high-throughput screening. Herein, we demonstrate the use of a microarray chip-based platform to screen, in high-throughput, individual and paired effects of 12 soluble factors on the neuronal differentiation of a human neural progenitor cell line (ReNcell VM) encapsulated in microscale 3D Matrigel cultures. Dose-response analysis of selected combinations from the initial combinatorial screen revealed that the combined treatment of all-trans retinoic acid (RA) with the glycogen synthase kinase 3 inhibitor CHIR-99021 (CHIR) enhances neurogenesis while simultaneously decreases astrocyte differentiation, whereas the combined treatment of brain-derived neurotrophic factor and the small azide neuropathiazol enhances the differentiation into neurons and astrocytes. Subtype specification analysis of RA- and CHIR-differentiated cultures revealed that enhanced neurogenesis was not biased toward a specific neuronal subtype. Together, these results demonstrate a high-throughput screening platform for rapid evaluation of differentiation conditions in a 3D environment, which will aid the development and application of 3D stem cell culture models.

show abstract

“…One of the earliest manifestations of a vitamin A-deficient diet is impaired vision, and it is from this, and the discovery of vitamin A derivatives in the retina, that retin-oic acid receives its name (Janesick et al., 2015). Many studies commonly use vitamin A-deficient (VAD) diets to disrupt and examine RA signaling.…”

Section: Main Textmentioning

confidence: 99%

Proliferation versus Differentiation: Redefining Retinoic Acid’s Role

Mosher

Schaffer

2018

Stem Cell Reports

View full text Add to dashboard Cite

show abstract

Retinoic acid signaling and neuronal differentiation

Abstract: The identification of neurological symptoms caused by vitamin A deficiency pointed to a critical, early developmental role of vitamin A and its metabolite, reti-

Cited by 235 publications

References 257 publications

Estrogen‐related receptor gamma regulates dopaminergic neuronal phenotype by activating GSK3β/NFAT signaling in SH‐SY5Y cells

Estrogen‐related receptor gamma regulates dopaminergic neuronal phenotype by activating GSK3β/NFAT signaling in SH‐SY5Y cells

High‐throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture

Proliferation versus Differentiation: Redefining Retinoic Acid’s Role

Contact Info

Product

Resources

About