Retinoic acid differentially regulates interleukin-1β and interleukin-1 receptor antagonist production by human alveolar macrophages

Hashimoto, Shigeo; Hayashi, S.; Yoshida, Sachiko; Kujime, Kosei; Maruoka, Shuichiro; Matsumoto, Ken; Gon, Yasuhiro; Koura, Toshiya; Horie, Takashi

doi:10.1016/s0145-2126(98)00119-2

Cited by 29 publications

(15 citation statements)

References 21 publications

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“…In our study vitamin A reduced the secretion of IL-1ra and exerted no effect on the production of IL-1 ␤ by cells from both age groups indicating a differential regulation of the vitamin on these cytokines. In line with our findings, differential regulation of IL-1 ␤ and IL-1ra production by RA has been described previously in human monocytes [26] and alveolar macrophages [27] stimulated with either phorbol myristate acetate or LPS. The authors have observed an inhibition of IL-1ra and a stimulation of IL-1 ␤ production in both types of cells.…”

Section: Discussionsupporting

confidence: 93%

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

Bessler¹,

Wyshelesky²,

Osovsky³

et al. 2006

Neonatology

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Background: For a long time vitamin A has been known to be essential for immune defense of the organism and protection against infections. Vitamin A deficiency in children is associated with morbidity and mortality from infectious diseases which could be prevented and even alleviated by vitamin A supplementation. Moreover, this vitamin is involved in the modulation of immunological and inflammatory responses by regulation of cytokine production. The aim of the study was to compare the in vitro effect of vitamin A on the production of pro-inflammatory (IL-1β and IL-6) and anti-inflammatory (IL-1 receptor antagonist (ra) and IL-10) cytokines, as well as IL-2 and IFNγ by cord blood mononuclear cells (CBMC) of preterm newborns to that of peripheral blood mononuclear cells (PBMC) from adults. Methods: Mononuclear cells (MC) from individuals of the two age groups were incubated with vitamin A (retinyl palmitate) at various concentrations in the presence of phytohemagglutinin for IL-2 and IFNγ production or LPS for IL-1β, IL-1ra, IL-6 and IL-10 secretion. The level of the cytokines in the supernatants was tested by ELISA. Results: Vitamin A exerted an in vitro inhibitory effect on the production of the anti-inflammatory cytokine IL-1ra by MC of preterm newborns and adults, but did not affect the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and IFNγ. Vitamin A caused inhibition of IL-10 secretion by cells from adults, but it did not significantly affect this function in cells from newborns except when high unphysiological doses were applied. In addition vitamin A stimulated the secretion of IL-2 by cells isolated from adults but had no effect on those derived from premature neonates. Conclusions: The results indicate that vitamin A may affect the immune function of premature infants via inhibition of IL-1ra secretion. It is suggested that the beneficial effect of vitamin A on the clinical course of bronchopulmonary dysplasia (BPD) may be due to the reduced production of anti-inflammatory cytokines by neonatal CBMC. This may indicate the importance of the pro-inflammatory cytokines in the management of severe lung diseases and BPD.

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Section: Discussionsupporting

confidence: 93%

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

Bessler¹,

Wyshelesky²,

Osovsky³

et al. 2006

Neonatology

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“…For example, up-regulation of proinflammatory mediators by RA such as IL-1 g , IL-8 and TNF- § has been reported previously [45][46][47], and these cytokines are also substantially promoted by ATRA in human mast cells (Babina et al, manuscript in preparation). Since IL-1 g and TNF- § are known to up-regulate ICAM-1 expression by EC, downregulation of CD11 subunits by monocytes might also represent a compensatory regulatory mechanism, preventing exaggerated adhesion of monocytes to the inflamed endothelium.…”

Section: Discussionmentioning

confidence: 76%

All‐trans retinoic acid down‐regulates expression and function of β₂ integrins by human monocytes: opposite effects on monocytic cell lines

Babina

Henz

2003

Eur J Immunol

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All-trans retinoic acid (ATRA) plays an important role in the differentiation of malignant myeloid cells but its effects on primary leukocytes have been poorly investigated. We report here that ATRA negatively affects expression and function of leukocyte integrins that play a key role in monocyte adhesive interactions. As evidenced by flow cytometry, ATRA (at 1 ? M) clearly and donor-independently suppressed the expression of all integrin chains investigated (CD11a, CD11b, CD11c, and CD18), most strikingly of CD11a. Down-regulation was detectable after 24 and maximal after 72-96 h. Reverse transcription-PCR analysis revealed diminished steady-state concentrations of § specific transcripts but not of the common g chain, suggesting that heterodimer expression was predominantly regulated through § chains. Results obtained with blood-derived monocytes were in sharp contrast to those for the leukemic cell lines THP-1 and U937, both of which showed marked increase in all integrin subunits in response to ATRA. ATRA-pretreated monocytes displayed significantly diminished g 2 integrin-dependent homotypic aggregation, and adhesion to stimulated endothelial cells (EC), while ATRA-pretreated monocytic cell lines showed the opposite behavior displaying markedly enhanced aggregation and CD18-mediated adhesion to EC. Therefore, the level of leukocyte integrins was obviously a decisive factor for these adhesive interactions irrespective of the cellular source. Collectively, our data indicate a striking difference between leukemic cell lines and normal hematopoietic cells with regard to ATRA responsiveness. By acting on key adhesive structures of normal leukocytes, ATRA mediates processes that may be of substantially broad range applying to inflammation and immunity in addition to differentiation and proliferation.

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“…An earlier report has showed that all- trans -retinoic acid (atRA) induces the production and secretion of IL-1β and the downregulation of IL-1ra in human alveolar macrophages [18]. The major objective of the present study is to determine the effect of atRA on the production and release of IL-1 from aortic SMC (AOSMC).…”

Section: Introductionmentioning

confidence: 94%

Expression of IL-1β, IL-1 Receptor Type I and IL-1 Receptor Antagonist in Human Aortic Smooth Muscle Cells: Effects of all-<i>trans</i>-Retinoic Acid

Wågsäter

Jatta²,

Ocaya³

et al. 2006

J Vasc Res

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The proinflammatory cytokine interleukin (IL)-1β and the IL-1 receptor antagonist are expressed by atherosclerotic plaques and may be linked to the development of atherosclerosis. Existing evidence shows that retinoids and their receptors are involved in inflammatory response and that they are found in atherosclerotic plaques. In all-trans-retinoic acid (atRA)-treated human aortic smooth muscle cells (AOSMC), significant increases in IL-1β levels were observed, compared with untreated cells. Examination of IL-1 receptor antagonist and IL-1 receptor type I levels did not show any difference between atRA-treated and -untreated AOSMC. The results show that atRA-treated AOSMC express both the precursor (33 kDa) and the active form (17 kDa) of the IL-1β protein. atRA-treated carotid lesions showed significantly elevated IL-1β mRNA levels (2.9 ± 2.33) compared with untreated lesions (2.0 ± 1.77; p < 0.05). These results support the role of atRA as a regulator of inflammation such as in atherosclerosis.

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Retinoic acid differentially regulates interleukin-1β and interleukin-1 receptor antagonist production by human alveolar macrophages

Cited by 29 publications

References 21 publications

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

All‐trans retinoic acid down‐regulates expression and function of β₂ integrins by human monocytes: opposite effects on monocytic cell lines

Expression of IL-1β, IL-1 Receptor Type I and IL-1 Receptor Antagonist in Human Aortic Smooth Muscle Cells: Effects of all-<i>trans</i>-Retinoic Acid

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Retinoic acid differentially regulates interleukin-1β and interleukin-1 receptor antagonist production by human alveolar macrophages

Cited by 29 publications

References 21 publications

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

A Comparison of the Effect of Vitamin A on Cytokine Secretion by Mononuclear Cells of Preterm Newborns and Adults

All‐trans retinoic acid down‐regulates expression and function of β2 integrins by human monocytes: opposite effects on monocytic cell lines

Expression of IL-1β, IL-1 Receptor Type I and IL-1 Receptor Antagonist in Human Aortic Smooth Muscle Cells: Effects of all-<i>trans</i>-Retinoic Acid

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All‐trans retinoic acid down‐regulates expression and function of β₂ integrins by human monocytes: opposite effects on monocytic cell lines