Retinal stem cell transplantation: Balancing safety and potential

Singh, Mandeep S.; Park, Susanna Soon Chun; Albini, Thomas A.; Canto‐Soler, M. Valeria; Klassen, Henry; MacLaren, Robert E.; Takahashi, Masayo; Nagiel, Aaron; Schwartz, Steven D.; Bharti, Kapil

doi:10.1016/j.preteyeres.2019.100779

Cited by 152 publications

(197 citation statements)

References 255 publications

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“…Stem cell engineering has opened a new avenue for repairing damaged nervous tissues. Following transplantation of cultured stem cells into eyes, they further integrate into the retinal microenvironment, and then proliferate and differentiate into target cells, to regenerate damaged neurons [6]. This offers recovery and reconstruction of the retinal function, with opportunities to treat irreversible blindness ophthalmopathy.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the RSC as the most suitable seed cell, we further examined the feasibility of RSC transplantation for treatment of damaged retina. In general, there are currently two types of transplantation methods: subretinal space injection and vitreous cavity injection [6,32]. Maintaining intraocular structure and preventing immune rejection is crucial for the survival of transplanted cells.…”

Section: Discussionmentioning

confidence: 99%

“…The intravitreal injection is a simplest feasible way in clinical practice for intraocular medication, which allows the eyeball to remain intact and reduces the occurrence of damage to the retina barrier. Moreover, this method offers a clear eld of view during operation [6,32,33]. Although both of these grafting methods are capable of integrating seed cells into the retina, some studies recently compared the two methods, showing that trauma induced by the subretinal injection is considerably greater leading to retinal detachment [17].…”

Section: Discussionmentioning

confidence: 99%

“…Three classes of stem or progenitor cells are utilized for cell therapy, including pluripotent stem cells (PSCs), fetal cells, and postnatal/adult cells. Cell-based therapies for retinal diseases that are currently under investigation usually use PSCs either embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) [3,4,[6][7][8][9]. To date, a lot of studies have shown that stem cells or stem-cell-derived cells improve the survival and function of host cells, not by producing the missing cells, but by secreting growth factors [10].…”

Section: Introductionmentioning

confidence: 99%

“…Therapeutic cells can be delivered to the intravitreal space or the subretinal space. However, subretinal injection posed a greater risk, which can result in retinal detachment [6].…”

Section: Introductionmentioning

confidence: 99%

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Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

Feng

Chen

Zhao

et al. 2020

Preprint

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Background: Stem cell transplantation has been reported as one of the promising strategies to treat retinal degenerative diseases, but this field is so complicated and still relatively new. This study investigated the potential role of transplantation of the embryo-derived retina stem cells (RSCs) into the vitreous cavity in repairing the damaged retina in mice.Methods: RSCs were isolated from Kunming mice E17 embryonic retina and ciliary body tissues. RSCs were cultured, differentiated, and labeled with 5-bromo-2’-deoxyuridin (BrdU). Retinal injury was induced in left eyes in male Kunming mice by ring clamping of the optic nerve. The 6th-generation of BrdU-labeled RSCs were transplanted into the damaged retina by the intravitreal injection, and saline injected eyes were used as the control. Hematoxylin and eosin histological staining, and BrdU, Nestin and Pax6 immunostaining were performed.Results: Embryo-derived RSCs were identified by the positive stains of Pax6 and Nestin. BrdU incorporation was detected in most of RSCs. The damaged retina showed diffuse edema, disordered and loose-arranged morphological changes in all retinal layers. In the damaged retina with RSCs transplantation, the positive staining for BrdU, Pax6 and Nestin were revealed on the retinal surface. Notably, a small amount of RSCs migrated into the retinal ganglion cell layer and inner nuclear.Conclusions: Embryonic RSCs have similar characteristics to neural stem cells. Transplantation of RSCs by intravitreal injection would be able to repair the damaged retina.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Therapeutic cells can be delivered to the intravitreal space or the subretinal space. However, subretinal injection posed a greater risk, which can result in retinal detachment [6].…”

Section: Introductionmentioning

confidence: 99%

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Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

Feng

Chen

Zhao

et al. 2020

Preprint

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AI and ML for Development of Cell and Gene Therapy for Personalized Treatment

Mhatre¹,

Shukla²,

Chavda³

et al. 2023

Bioinformatics Tools for Pharmaceutical Drug Product Development

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Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration

Jiang

Shao

et al. 2022

Bioengineering & Transla Med

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Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal‐derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self‐renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold‐free 3D aggregates using light‐controlled cell sheet technology and a serum‐free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate‐implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.

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Retinal stem cell transplantation: Balancing safety and potential

Cited by 152 publications

References 255 publications

Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

Transplanted Embryonic Retinal Stem Cells have the Potential to Repair the Injured Retina in Mice

AI and ML for Development of Cell and Gene Therapy for Personalized Treatment

Light‐controlled scaffold‐ and serum‐free hard palatal‐derived mesenchymal stem cell aggregates for bone regeneration

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