Retinal proliferation response in the buphthalmic zebrafish, bugeye

Lama-Sherpa, Tshering; Hunter, Samuel S.; Frey, Ruth A.; Robison, Barrie D.; Stenkamp, Deborah L.

doi:10.1016/j.exer.2011.06.001

Cited by 20 publications

(17 citation statements)

References 53 publications

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“…To determine the time‐course of functional recovery after a selective retinal injury that spares photoreceptors as well as Müller glia, we used two complementary behavioral assays: the DLR assay (Sherpa et al, ), and a place‐preference assay (Sherpa et al, ). Fourteen fish were subjected to unilateral, intraocular injection of 2 µ M ouabain, and the DLR was monitored during recovery.…”

Section: Resultsmentioning

confidence: 99%

“…Briefly, three sections from each eye, containing the ONH, were stained with 0.1% of methylene blue and brightfield images were collected. Scion Image/Fiji software (Schindelin et al, ) was used to measure the diameter of the ONH (Sherpa et al, ). For each section, the averages of three replicate measurements of ONH diameter were recorded.…”

Section: Methodsmentioning

confidence: 99%

“…Measurement of ONHs. ONHs were measured as described previously (Fimbel et al, 2007;Sherpa et al, 2008;Sherpa et al, 2011). Briefly, three sections from each eye, containing the ONH, were stained with 0.1% of methylene blue and brightfield images were collected.…”

Section: Histological Methodsmentioning

confidence: 99%

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Retinal regeneration is facilitated by the presence of surviving neurons

Lama-Sherpa

Lankford

McGinn

et al. 2014

Developmental Neurobiology

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108

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Teleost fish regenerate their retinas after damage, in contrast to mammals. In zebrafish subjected to an extensive ouabain-induced lesion that destroys all neurons and spares Müller glia, functional recovery and restoration of normal optic nerve head (ONH) diameter take place at 100 days post-injury. Subsequently, regenerated retinas overproduce cells in the retinal ganglion cell (RGC) layer, and the ONH becomes enlarged. Here we test the hypothesis that a selective injury, which spares photoreceptors and Müller glia, results in faster functional recovery and fewer long-term histological abnormalities. Following this selective retinal damage, recovery of visual function required 60 days, consistent with this hypothesis. In contrast to extensively damaged retinas, selectively damaged retinas showed fewer histological errors and did not overproduce neurons. Extensively damaged retinas had RGC axons that were delayed in pathfinding to the ONH, and showed misrouted axons within the ONH, suggesting that delayed functional recovery following an extensive lesion is related to defects in RGC axons exiting the eye and/or reaching their central targets. The atoh7, fgf8a, shha, and netrin-1 genes were differentially expressed, and the distribution of Hh protein was disrupted following extensive damage as compared with selective damage. Confirming a role for Shh signaling in supporting rapid regeneration, shhat4+/− zebrafish showed delayed functional recovery following selective damage. We suggest that surviving retinal neurons provide structural/molecular information to regenerating neurons, and that this patterning mechanism regulates factors such as Shh. These factors in turn control neuronal number, retinal lamination, and RGC axon pathfinding during retinal regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Retinal regeneration is facilitated by the presence of surviving neurons

Lama-Sherpa

Lankford

McGinn

et al. 2014

Developmental Neurobiology

Self Cite

108

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“…Ocular growth and retinal stretching also modulate the rate of proliferation of rod precursors. In both goldfish (Raymond et al, 1988a) and zebrafish (Sherpa et al, 2011) mutant strains have been isolated that have grossly enlarged eyes due to elevated intraocular pressure (a phenotype related to congenital glaucoma in humans, or buphthalmia); in these fish the number of 3 H-thymidine or BrdU-labeled rod precursors is increased, indicating a larger population that generates more rod photoreceptors.…”

Section: Rod Photoreceptor Lineage In Teleost Fishmentioning

confidence: 99%

Müller glia: Stem cells for generation and regeneration of retinal neurons in teleost fish

Lenkowski

Raymond

2014

Progress in Retinal and Eye Research

285

303

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Adult zebrafish generate new neurons in the brain and retina throughout life. Growth-related neurogenesis allows a vigorous regenerative response to damage, and fish can regenerate retinal neurons, including photoreceptors, and restore functional vision following photic, chemical, or mechanical destruction of the retina. Müller glial cells in fish function as radial-glial-like neural stem cells. During adult growth, Müller glial nuclei undergo sporadic, asymmetric, self-renewing mitotic divisions in the inner nuclear layer to generate a rod progenitor that migrates along the radial fiber of the Müller glia into the outer nuclear layer, proliferates, and differentiates exclusively into rod photoreceptors. When retinal neurons are destroyed, Müller glia in the immediate vicinity of the damage partially and transiently dedifferentiate, re-express retinal progenitor and stem cell markers, re-enter the cell cycle, undergo interkinetic nuclear migration (characteristic of neuroepithelial cells), and divide once in an asymmetric, self-renewing division to generate a retinal progenitor. This daughter cell proliferates rapidly to form a compact neurogenic cluster surrounding the Müller glia; these multipotent retinal progenitors then migrate along the radial fiber to the appropriate lamina to replace missing retinal neurons. Some aspects of the injury-response in fish Müller glia resemble gliosis as observed in mammals, and mammalian Müller glia exhibit some neurogenic properties, indicative of a latent ability to regenerate retinal neurons. Understanding the specific properties of fish Müller glia that facilitate their robust capacity to generate retinal neurons will inform and inspire new clinical approaches for treating blindness and visual loss with regenerative medicine.

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“…There is evidence that Müller glia must participate in phagocytosis of apoptotic bodies in damaged retina in order to proliferate (Bailey et al, 2011). However, in a zebrafish model for chronic retinal cell stress (the lrp2−/− fish, bugeye ), extensive cell death is not required for a robust proliferative response (Sherpa et al, 2011). A current approach in our laboratory utilizes a comparative evaluation of the retinal response to damage in zebrafish vs. the retinal response to damage in mouse.…”

Section: Plasticity Of the Rod Lineagementioning

confidence: 99%

The rod photoreceptor lineage of teleost fish

Stenkamp

2011

Progress in Retinal and Eye Research

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The retinas of postembryonic teleost fish continue to grow for the lifetime of the fish. New retinal cells are added continuously at the retinal margin, by stem cells residing at the circumferential germinal zone. Some of these retinal cells differentiate as Müller glia with cell bodies that reside within the inner nuclear layer. These glia retain some stem cell properties in that they carry out asymmetric cell divisions and continuously generate a population of transit-amplifying cells – the rod photoreceptor lineage – that are committed to rod photoreceptor neurogenesis. These rod progenitors progress through a stereotyped sequence of changes in gene expression as they continue to divide and migrate to the outer nuclear layer. Now referred to as rod precursors, they undergo terminal mitoses and then differentiate as rods, which are inserted into the existing array of rod and cone photoreceptors. The rod lineage displays developmental plasticity, as rod precursors can respond to the loss of rods through increased proliferation, resulting in rod replacement. The stem cells of the rod lineage, Müller glia, respond to acute damage of other retinal cell types by increasing their rate of proliferation. In addition, the Müller glia in an acutely damaged retina dedifferentiate and become multipotent, generating new, functional neurons. This review focuses on the cells of the rod lineage and includes discussions of experiments over the last 30 years that led to their identification and characterization, and the discovery of the stem cells residing at the apex of the lineage. The plasticity of cells of the rod lineage, their relationships to cone progenitors, and the applications of this information for developing future treatments for human retinal disorders will also be discussed.

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Retinal proliferation response in the buphthalmic zebrafish, bugeye

Cited by 20 publications

References 53 publications

Retinal regeneration is facilitated by the presence of surviving neurons

Retinal regeneration is facilitated by the presence of surviving neurons

Müller glia: Stem cells for generation and regeneration of retinal neurons in teleost fish

The rod photoreceptor lineage of teleost fish

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