Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells

Vossmerbaeumer, Urs; Ohnesorge, Stefanie; Kuehl, Sandra; Haapalahti, Minna; Klüter, Harald; Jonas, Jost B.; Thierse, Hermann‐Josef; Bieback, Karen

doi:10.1080/14653240802714819

Cited by 70 publications

(68 citation statements)

References 33 publications

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“…SVCs lacking CD34 can form epithelial cells in vitro in response to all-trans retinoic acid ( 47 ). A related study shows that ASCs can differentiate in vitro into cells resembling retinal pigment epithelial cells, with the ability to form pigmented granula ( 168 ). Moreover, CD34-SVCs can engraft into retinas in a model of diabetic retinopathy ( 169 ), suggesting another potential therapeutic application of these cells.…”

Section: Ectodermal Cellsmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

356

323

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with ill health. Such stigmatization distracts from a fascinating aspect of WAT biology: its enormous plasticity as an organ. Indeed, WAT is capable of massive expansion and contraction in response to chronic alterations in energy balance, accounting for as little as 5% body mass in extremely lean athletes ( 1 ) or as much as 60% body mass in morbidly obese individuals ( 2 ). Moreover in rodents, rabbits, and humans, WAT can regenerate following lipectomy ( 3-6 ). This striking degree of plasticity is unique among organs in adults. On a cellular level, WAT expansion is driven by both hypertrophy and hyperplasia of adipocytes ( 7-13 ). Even in nonexpanding WAT, adipocytes renew frequently to compensate for adipocyte death, with approximately 10% of adipocytes renewed annually ( 14,15 ). These data, which indicate that committed adipocyte progenitors (preadipocytes) exist within WAT, are the product of decades of research motivated largely by our desire to understand adipose tissue in the context of obesity and related diseases. Preadipocytes exist in adipose tissueThough the notion of obesity as a disease has existed since at least the time of Hippocrates ( 16 ), investigation into the origins of the adipocyte and the individual contributions of adipocyte hypertrophy and hyperplasia to adipose bulk began in earnest only after the appearance of the stem cell concept, as related to blood cells, in the early 1900s ( 17 ). In the 1940s, histologic observations of the appearance of adipocytes in chambers implanted in the ears of live rabbits suggested that de novo fat formation was Abstract White adipose tissue (WAT) is perhaps the most plastic organ in the body, capable of regeneration following surgical removal and massive expansion or contraction in response to altered energy balance. Research conducted for over 70 years has investigated adipose tissue plasticity on a cellular level, spurred on by the increasing burden that obesity and associated diseases are placing on public health globally. This work has identifi ed committed preadipocytes in the stromal vascular fraction of adipose tissue and led to our current understanding that adipogenesis is important not only for WAT expansion, but also for maintenance of adipocyte numbers under normal metabolic states. At the turn of the millenium, studies investigating preadipocyte differentiation collided with developments in stem cell research, leading to the discovery of multipotent stem cells within WAT. Such adipose tissue-derived stem cells (ASCs) are capable of differentiating into numerous cell types of both mesodermal and nonmesodermal origin, leading to their extensive investigation from a therapeutic and tissue engineering perspective. However, the insights gained through studying ASCs have also contributed to more-recent progress in attempts to better characterize committed preadipocytes in adipose tissue. Thus, ASC research has gone back to its roots, thereby expanding our knowledge of preadipocyte commitment and adipose tissue biology. -Cawthorn, W. P., E. ...

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Section: Ectodermal Cellsmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

356

323

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“…107 Various stem cells such as embryonic stem cells, [108][109][110] hematopoietic stem cells, [111][112][113] mesenchymal stem cells (MSCs), [114][115][116] induced pluripotent stem cells, 117,118 and EPC 119,120 have been shown to demonstrate the capability to regenerate into retinal neurons, 108,[110][111][112][113]117 retinal pigmented epithelium, 109,121,122 and improve blood vessels permeability 123 in both in vitro and in vivo studies. It is noteworthy that the transplanted cells will need to survive the harsh microenvironment before cell programming can occur for the replacement of damaged tissue.…”

Section: Future Directionsmentioning

confidence: 99%

Revisiting the role of erythropoietin for treatment of ocular disorders

Ding

Leow

Munisvaradass

et al. 2016

Eye

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Erythropoietin (EPO) is a glycoprotein hormone conventionally thought to be responsible only in producing red blood cells in our body. However, with the discovery of the presence of EPO and EPO receptors in the retinal layers, the EPO seems to have physiological roles in the eye. In this review, we revisit the role of EPO in the eye. We look into the biological role of EPO in the development of the eye and the physiologic roles that it has. Apart from that, we seek to understand the mechanisms and pathways of EPO that contributes to the therapeutic and pathological conditions of the various ocular disorders such as diabetic retinopathy, retinopathy of prematurity, glaucoma, age-related macular degeneration, optic neuritis, and retinal detachment. With these understandings, we discuss the clinical applications of EPO for treatment of ocular disorders, modes of administration, EPO formulations, current clinical trials, and its future directions.

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“…Interestingly, it has been suggested that rat MSCs can be made to express photopigment (rhodopsin) in vitro simply by adding epidermal growth factor to the culture media (Zhang, 2008). Additionally, though other retina-relevant cell types have been engineered, a number of studies have shown that BM or adipose tissue MSCs are converted to RPE (Gong, 2008;Arnhold, 2006;Vossmerbaeumer 2009). As with work on other neuronal phenotypes, however, there has now been a reassessment of the ability of MSCs to differentiate into functionally useful retinal cells.…”

Section: Multipotent Mesenchymal Stromal Cells (Mesenchymal Stem Cells)mentioning

confidence: 99%

Stem-Cell Therapy for Retinal Diseases

Siqueira¹

2011

Embryonic Stem Cells - Differentiation and Pluripotent Alternatives

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Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells

Cited by 70 publications

References 33 publications

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Revisiting the role of erythropoietin for treatment of ocular disorders

Stem-Cell Therapy for Retinal Diseases

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