Retinal hypoxia after experimental subarachnoid hemorrhage

Zhaba, Wang-Dui; Deji, Qu-Zhen; Deng, Hong-Ji; Han, Yanling; Gao, Sheng-Qing; Li, Xilin; Zhou, Meng-Liang

doi:10.1016/j.neulet.2020.135554

Cited by 3 publications

(6 citation statements)

References 24 publications

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“…2a,b). The increased HIF-1α and VEGF-A in the SAH group were consistent with the results of new vessels in the retina in response to SAH in our previous study [13].…”

Section: Induction Of the Hif-1α/vegf-a Pathway In The Subarachnoid H...supporting

confidence: 91%

“…Motor abnormalities, intellectual disabilities and visual disorders are especially common in patients after SAH [9]. In our previous study, we conducted a morphological and functional study of the retina after SAH and observed a large number of new vessels in the retina after SAH [13]. This phenomenon has been studied in diabetic retinopathy and retinopathy of prematurity [15].…”

Section: Discussionmentioning

confidence: 99%

“…Retinal vessels, which are an embryological part of the intracranial vasculature, are easily accessible for analysis. In our previous study, the following changes in hypoxia-related elements were identified in the retina after SAH [13]. In the present study, we investigated the effects of deferoxamine on retinal hypoxia after SAH.…”

Section: Introductionmentioning

confidence: 86%

“…Adult male Sprague–Dawley rats [ n = 141 (135 + 6 {dead}); age, 6–8 weeks; weight, 250–300 g] from the Animal Center of Jinling Hospital (Nanjing, China) were used in this study. SAH model was performed as previously described [13]. The animals were randomly divided into a control group and five SAH groups ( n = 9 in each group) to get the time-course expression of HIF-1α and VEGF-A in the retina by western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The immunohistochemistry was performed as previously described [13], and primary antibodies against CD31 (ab28364; Abcam) or HIF-1α (ab2185; Abcam) were used.…”

Section: Immunohistochemical and Hematoxylin And Eosin Stainingmentioning

confidence: 99%

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Activation of HIF-1α/VEGF-A pathway by deferoxamine ameliorates retinal hypoxia in a rat subarachnoid hemorrhage model

et al. 2022

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Background and purpose Subarachnoid hemorrhage (SAH) is associated with sustained vasoconstriction in retinal vessels and vasoconstriction leads to retinal ischemia and hypoxia. Our previous finding also revealed the changes in hypoxia-related elements in the retina after SAH, further lending weight to the hypothesis that retinal vasospasm and hypoxia after SAH. Deferoxamine is a high-affinity iron chelator with reported neuroprotective effects against stroke. Here, we aimed to explore the effects of deferoxamine on retinal hypoxia after SAH. Methods SAH was established and deferoxamine was injected intraperitoneally for 3 days in the treatment group. To detect retinal new vessels, platelet endothelial cell adhesion molecule (CD31) was labeled by immunofluorescence and immunohistochemistry. Furthermore, the effects of deferoxamine on the expression of vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α) were revealed by western blot analysis. Results The immunofluorescence and immunohistochemical staining of CD31 revealed a marked increase in new vessels in the retinal ganglion cell layer after deferoxamine treatment. By western blot analysis, HIF-1α and VEGF-A increased gradually in the first day and then rebounded to a new level on day 7. A deferoxamine-induced increase in HIF-1α/VEGF-A expression was also confirmed by western blot. Conclusions Our findings suggest that modulating the application of deferoxamine may offer therapeutic approaches to alleviate retinal complications after SAH.

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“…2a,b). The increased HIF-1α and VEGF-A in the SAH group were consistent with the results of new vessels in the retina in response to SAH in our previous study [13].…”

Section: Induction Of the Hif-1α/vegf-a Pathway In The Subarachnoid H...supporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

“…The immunohistochemistry was performed as previously described [13], and primary antibodies against CD31 (ab28364; Abcam) or HIF-1α (ab2185; Abcam) were used.…”

Section: Immunohistochemical and Hematoxylin And Eosin Stainingmentioning

confidence: 99%

See 3 more Smart Citations