Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy

Bueno, Juan M.; Palacios, Raquel; Giakoumaki, Anastasia; Gualda, Emilio J.; Schaeffel, Frank; Artal, Pablo

doi:10.1364/boe.5.000664

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Recently, single retinal photoreceptors were imaged in the living chick eye using adaptive optics (AO) retinal imaging systems (Headington, et al, 2011, Kisilak, et al, 2012, Bueno, et al, 2014). In this study, AO retinal imaging was used in conjunction with the cone pointing measurement technique of Roorda and Williams (Roorda & Williams, 2002) to determine the pointing of individual chick photoreceptors.…”

Section: Introductionmentioning

confidence: 99%

Measurement of the photoreceptor pointing in the living chick eye

et al. 2015

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The chick eye is used in the study of ocular growth and emmetropization; however optical aberrations in the lens and cornea limit the ability to visualize fine retinal structure in living eyes. These aberrations can be corrected using adaptive optics (AO) allowing for cellular level imaging in-vivo. Here, this capability is extended to measure the angular tuning properties of individual photoreceptors. The left eyes from two White Leghorn chicks (gallus gallus domestie beled) chick A and chick B, were imaged using an AO flood illuminated fundus camera. By translating the entrance pupil position, the same retinal location was illuminated with light of varying angles allowing for the measurement of individual photoreceptor pointing. At 30° nasal from the pecten tip, the pointing direction for both chicks was towards the pupil center with a narrow distribution. These particul chicks were found to have a temporal (T) and inferior (I) bias in the alignment with peak positions of (0.81 T, 0.23 I) and (0.57 T, 0.18 I) mm from the pupil center for chicks A and B respectively. The rho, ρ, values for the major, ρl, and minor, ρs, axes were 0.14 and 0.17 mm−2 for chick A and 0.09 and 0.20 mm−2 for chick B. The small disarray in the alignment of the chick photoreceptors implies that the photoreceptors are aligned to optimize the light entering the eye through the central portion of the pupil aperture. The ability to measure pointing properties of individual photoreceptors will have application in the study of eye growth and various retinal disorders.

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Section: Introductionmentioning

confidence: 99%

Measurement of the photoreceptor pointing in the living chick eye

et al. 2015

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The Role of Retinal Dysfunction in Myopia Development

et al. 2022

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In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue

et al. 2016

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High resolution multiphoton tomography and fluorescence lifetime imaging differentiates glioma from adjacent brain in native tissue samples ex vivo. Presently, multiphoton tomography is applied in clinical dermatology and experimentally. We here present the first application of multiphoton and fluorescence lifetime imaging for in vivo imaging on humans during a neurosurgical procedure. We used a MPTflex™ Multiphoton Laser Tomograph (JenLab, Germany). We examined cultured glioma cells in an orthotopic mouse tumor model and native human tissue samples. Finally the multiphoton tomograph was applied to provide optical biopsies during resection of a clinical case of glioblastoma. All tissues imaged by multiphoton tomography were sampled and processed for conventional histopathology. The multiphoton tomograph allowed fluorescence intensity- and fluorescence lifetime imaging with submicron spatial resolution and 200 picosecond temporal resolution. Morphological fluorescence intensity imaging and fluorescence lifetime imaging of tumor-bearing mouse brains and native human tissue samples clearly differentiated tumor and adjacent brain tissue. Intraoperative imaging was found to be technically feasible. Intraoperative image quality was comparable to ex vivo examinations. To our knowledge we here present the first intraoperative application of high resolution multiphoton tomography and fluorescence lifetime imaging of human brain tumors in situ. It allowed in vivo identification and determination of cell density of tumor tissue on a cellular and subcellular level within seconds. The technology shows the potential of rapid intraoperative identification of native glioma tissue without need for tissue processing or staining.

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Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy

Cited by 5 publications

References 45 publications

Measurement of the photoreceptor pointing in the living chick eye

Measurement of the photoreceptor pointing in the living chick eye

The Role of Retinal Dysfunction in Myopia Development

In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue

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