Retina derived relaxation is mediated by Kir channels and the inhibition of Ca2+ sensitization in isolated bovine retinal arteries

Takır, Selçuk; Uydeş-Doğan, B. Sönmez; Özdemir, Osman

doi:10.1016/j.exer.2015.02.003

Cited by 9 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…42 We tried to test this influence by blocking Rho-kinase with Y-27632 dihydrochlo- ride, but the incubation caused loss of contractile tone, which could be explained by the fact that Y-27632 dihydrochoride is a strong dilator of PGF 2a -contracted retinal arteries. 43 To be sure that the inhibition of the resveratrol relaxation by PMA or PDBu is not aspecific, experiments were carried out with papaverine, a strong dilator of bovine retinal arteries. 40 Relaxations in response to papaverine were not affected by PDBu (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…An induction of relaxation by activating inward rectifier K þ channels and an inhibition of contraction by inhibiting the Ca 2þ sensitization pathway probably via the Rho-kinase pathway was suggested. 43 Changes in the RRF pathway could affect the retinal blood flow. Therefore, in bioassay experiments we also investigated whether resveratrol influences the relaxing effect of RRF elicited by placing mouse retinal tissue on a precontracted mouse femoral artery.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries

Daele

Boydens

Pauwels

et al. 2016

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries

Daele

Boydens

Pauwels

et al. 2016

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…6 L-type Ca 2þ channels are also not involved, because blocking them with nifedipine did not change the RRF relaxation. 16 Surprisingly, blocking the plasma membrane Ca 2þ -adenosine triphosphate (ATP)ase (PMCA) with vanadate inhibited the RRF-induced relaxation (Fig. 1).…”

Section: Excluded Molecules Characteristicsmentioning

confidence: 95%

“…Blockade of sarco/endoplasmic reticulum Ca 2þ ATPase (SERCA) with thapsigargin and cyclopiazonic acid did not influence the RRF-induced relaxation. 6,16 Also, blocking the Na þ -Ca 2þ exchanger with dimethyl-2thiourea (DMTU) or amiloride had no influence. 6 L-type Ca 2þ channels are also not involved, because blocking them with nifedipine did not change the RRF relaxation.…”

Section: Excluded Molecules Characteristicsmentioning

confidence: 99%

“…Interestingly, the retinal adhering tissue together with Y-27632 can cause an almost complete relaxation in 120 mM K þ KRB-contracted arteries. 16 Potassium (K þ ) channels could be involved in the RRF relaxation, because bovine retinal arteries relax less when contracted with a 120 mM K þ KRB solution. 4 It even changes the biphasic RRF-induced relaxation into a monophasic one.…”

Section: Excluded Molecules Characteristicsmentioning

confidence: 99%

See 1 more Smart Citation

The Retinal Relaxing Factor: Update on an Enigmatic Regulator of the Retinal Circulation

Daele

Boydens

Pauwels

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

The retinal circulation is regulated by different local factors and might include the retinal relaxing factor (RRF). This factor is found to be continuously released by the retina and relaxes smooth muscle cells. This review describes the current knowledge about the RRF. Despite many research efforts, the cellular source, identity, mechanism, and physiological role of the RRF remain largely unknown. Thus far, it seems that the RRF is a hydrophilic, thermostable, diffusible chemical messenger, which characteristics do not correspond with most well-known endogenous vasorelaxants. The RRF-induced relaxation seems to rely on activation of the inward rectifier K+ channels and the Rho kinase Ca2+ sensitization mechanism. Voltage-dependent K+ channels and plasma membrane Ca2+-ATPase might also be involved, whereas the involvement of cyclooxygenase is still a point of discussion. Furthermore, it appears that the RRF is involved in other relaxation pathways, namely those of hypoxia, adenosine, and adenosine triphosphate, hydrogen sulfide, γ-aminobutyric acid, and dorzolamide.

show abstract

Cognitive impairment and microvascular function in end‐stage renal disease

Angermann

Günthner

Hanssen

et al. 2022

Int J Methods Psych Res

View full text Add to dashboard Cite

Objective Hemodialysis patients show an approximately threefold higher prevalence of cognitive impairment compared to the age‐matched general population. Impaired microcirculatory function is one of the assumed causes. Dynamic retinal vessel analysis is a quantitative method for measuring neurovascular coupling and microvascular endothelial function. We hypothesize that cognitive impairment is associated with altered microcirculation of retinal vessels. Methods 152 chronic hemodialysis patients underwent cognitive testing using the Montreal Cognitive Assessment. Retinal microcirculation was assessed by Dynamic Retinal Vessel Analysis, which carries out an examination recording retinal vessels' reaction to a flicker light stimulus under standardized conditions. Results In unadjusted as well as in adjusted linear regression analyses a significant association between the visuospatial executive function domain score of the Montreal Cognitive Assessment and the maximum arteriolar dilation as response of retinal arterioles to the flicker light stimulation was obtained. Conclusion This is the first study determining retinal microvascular function as surrogate for cerebral microvascular function and cognition in hemodialysis patients. The relationship between impairment in executive function and reduced arteriolar reaction to flicker light stimulation supports the involvement of cerebral small vessel disease as contributing factor for the development of cognitive impairment in this patient population and might be a target for noninvasive disease monitoring and therapeutic intervention.

show abstract

Retina derived relaxation is mediated by Kir channels and the inhibition of Ca2+ sensitization in isolated bovine retinal arteries

Cited by 9 publications

References 37 publications

Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries

Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries

The Retinal Relaxing Factor: Update on an Enigmatic Regulator of the Retinal Circulation

Cognitive impairment and microvascular function in end‐stage renal disease

Contact Info

Product

Resources

About